The effects of cholesterol and β-sitosterol on the structure of saturated diacylphosphatidylcholine bilayers

Gallová, Jana; Uhrı́ková, Daniela; Kučerka, Norbert; Doktorovová, Slavomíra; Funari, Sérgio S.; Teixeira, J.; Balgavý, P

doi:10.1007/s00249-010-0635-6

Cited by 28 publications

(16 citation statements)

References 46 publications

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“…In the range of experimental error, b-sitosterol has a similar effect on the thickness of EYPC bilayers as cholesterol. Comparable ability of cholesterol and b-sitosterol to increase the bilayer thickness of unilamellar vesicles was also observed in our recent reports on DMPC and DPPC bilayers (Gallová et al 2011) and diCn:1PC (1,2-monounsaturated diacyl-sn-glycero-3-phosphatidylcholine with n carbon atoms in the acyl chain) bilayers (n = 14-22) (Gallová et al 2008). A smaller ability of b-sitosterol in comparison to cholesterol to increase the thickness of hydrated phosphatidylcholine bilayers was reported by other techniques (Hodzic et al 2008;Krajewski-Bertrand et al 1992;Bernsdorff and Winter 2003;Hac-Wydro et al 2007).…”

Section: Resultssupporting

confidence: 86%

“…It is therefore not possible to show unequivocally whether studied sterols in the concentration range 17 B X B 41 mol% influence the water layer differently or not. A similar study using saturated phosphatidylcholine DMPC and DPPC showed that both sterols increased the bilayer thickness similarly but cholesterol made the water layer thinner than b-sitosterol (Gallová et al 2011). A reduction of the multilamellar water layer by sterols was observed also in multilamellar liposomes of POPC (Hodzic et al 2008) with cholesterol being more effective than b-sitosterol.…”

Section: Resultsmentioning

confidence: 72%

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Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

et al. 2011

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The influence of cholesterol and b-sitosterol on egg yolk phosphatidylcholine (EYPC) bilayers is compared. Different interactions of these sterols with EYPC bilayers were observed using X-ray diffraction. Cholesterol was miscible with EYPC in the studied concentration range (0-50 mol%), but crystallization of b-sitosterol in EYPC bilayers was observed at X C 41 mol% as detected by X-ray diffraction. Moreover, the repeat distance (d) of the lamellar phase was similar upon addition of the two sterols up to mole fraction 17%, while for X C 17 mol% it became higher in the presence of b-sitosterol compared to cholesterol. SANS data on suspensions of unilamellar vesicles showed that both cholesterol and b-sitosterol similarly increase the EYPC bilayer thickness. Cholesterol in amounts above 33 mol% decreased the interlamellar water layer thickness, probably due to ''stiffening'' of the bilayer. This effect was not manifested by b-sitosterol, in particular due to the lower solubility of b-sitosterol in EYPC bilayers. Applying the formalism of partial molecular areas, it is shown that the condensing effect of both sterols on the EYPC area at the lipid-water interface is small, if any. The parameters of ESR spectra of spin labels localized in different regions of the EYPC bilayer did not reveal any differences between the effects of cholesterol and b-sitosterol in the range of full miscibility.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 72%

Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

et al. 2011

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“…The necessity of improved safety of the currently developed drug delivery systems, aiming at reduced side effects, strongly stimulates the research on naturally occurring products, biomimetic and bio-inspired materials, and components. 1 − 12 Lipid-based nanostructured dispersions are well recognized as gentle and biocompatible drug delivery systems. 13 − 20 Cubosomes, hexosomes, spongosomes, or vesicular lipid assemblies are fabricated depending on the amphiphilic compositions and the employed dispersing agents.…”

Section: Introductionmentioning

confidence: 99%

Liquid Crystalline Nanostructures as PEGylated Reservoirs of Omega-3 Polyunsaturated Fatty Acids: Structural Insights toward Delivery Formulations against Neurodegenerative Disorders

et al. 2018

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Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are bioactive lipids with considerable impact in medicine and nutrition. These compounds exert structuring effects on the cellular membrane organization, regulate the gene expression, and modulate various signaling cascades and metabolic processes. The purpose of the present work is to demonstrate the structural features of ω-3 PUFA-containing three-dimensional supramolecular lipid assemblies suitable for pharmaceutical applications that require soft porous carriers. We investigate the liquid crystalline structures formed upon mixing of eicosapentaenoic acid (EPA, 20:5) with the lyotropic nonlamellar lipid monoolein and the formation of multicompartment assemblies. Starting with the monoolein-based lipid cubic phase, double membrane vesicles, cubosome precursors, sponge-type particles (spongosomes), mixed intermediate nonlamellar structures, and multicompartment assemblies are obtained through self-assembly at different amphiphilic compositions. The dispersions containing spongosomes as well as nanocarriers with oil and vesicular compartments are stabilized by PEGylation of the lipid/water interfaces using a phospholipid with a poly(ethylene glycol) chain. The microstructures of the bulk mixtures were examined by cross-polarized light optical microscopy. The dispersed liquid crystalline structures and intermediate states were studied by small-angle X-ray scattering, cryogenic transmission electron microscopy, and quasielastic light scattering techniques. They established that PUFA influences the phase type and the sizes of the aqueous compartments of the liquid crystalline carriers. The resulting multicompartment systems and stealth nanosponges may serve as mesoporous reservoirs for coencapsulation of ω-3 PUFA (e.g., EPA) with water-insoluble drugs and hydrophilic macromolecules toward development of combination treatment strategies of neurodegenerative and other diseases.

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“…As the spontaneous curvature of CHOL is more negative than that of phospholipids, 47 the solubilization of CHOL containing bilayers can be expected to require more surfactant with positive spontaneous curvature. According to previous studies, CHOL causes the ordering of EYPC acyl chains 48,49 and thickening of the bilayer 50 in its fluid state. This is associated with the increase of EYPC bilayer integrity and leads to higher D T SOL values.…”

Section: Resultsmentioning

confidence: 81%

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

Huláková

Gallová

Devı́nsky

2015

ACSi

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The interaction of N, N-dimethyl-1-dodecanamine N-oxide (C 12 NO) with egg yolk phosphatidylcholine (EYPC) liposomes containing cholesterol (CHOL) was studied. The perturbation of CHOL-EYPC bilayers in unilamellar liposomes (ULL) was observed by the leakage of fluorescence probe calcein. Weak leakage is observed at low surfactant concentration c C12NO (minimal perturbation of the bilayer) followed by an intensive leakage at a middle c C12NO (creation of pores). No change in fluorescence intensity was measured at high c C12NO (calcein totally released from liposomes). The higher CHOL amount in the bilayer, the more surfactant is needed to create pores in the bilayer. Solubilization of CHOL-EYPC ULL induced by C 12 NO was studied turbidimetrically. The solubilization curve consists of three parts: saturation of bilayer at low c C12NO (liposomes are preserved), followed by solubilization (liposome -mixed micelle transition) and post-solubilization. The C 12 NO concentration needed for the onset of the solubilization raise with the increase of n CHOL :n EYPC . The structure of liposomes is still preserved at total calcein release for all n CHOL :n EYPC .

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The effects of cholesterol and β-sitosterol on the structure of saturated diacylphosphatidylcholine bilayers

Cited by 28 publications

References 46 publications

Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

Liquid Crystalline Nanostructures as PEGylated Reservoirs of Omega-3 Polyunsaturated Fatty Acids: Structural Insights toward Delivery Formulations against Neurodegenerative Disorders

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

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