The effects of chitosan and salicylic acid on elicitation of secondary metabolites and antioxidant activity of safflower under in vitro salinity stress

Golkar, Pooran; Taghizadeh, Marzieh; Yousefian, Zeinab

doi:10.1007/s11240-019-01592-9

Cited by 80 publications

(46 citation statements)

References 40 publications

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“…This is probably due to the inhibitory effects of elicitors on cell growth and capacity of cell osmotic adjustment, which increase the requirement for maintaining turgor of the growing cells, consuming energy, and decrease in callus growth (Golkar et al 2019). Similarly, higher concentrations of other elicitors phenylalanine and mevalonic acid in Zingiber o cinale (El-Nabarawy et al 2015) and chitosan in sa ower (Golkar et al 2019) reduced the fresh weight of the callus. The CGR showed variation from 0.53 (mm day − 1 ) at 0.5 mM Pu to 0.17 (mm day − 1 ) under 100 µM MeJ (Table 3).…”

Section: Callus Growth Traitsmentioning

confidence: 99%

“…In agreement with these previous studies, exposure to MeJ signi cantly increased the MDA content in Panax ginseng. (Ali et al 2007) through suspension culture and other elicitors such as chitosan (Golkar et al 2019) through callus culture. According to the results, MDA content under 1 mM (Pu) was not signi cantly different from the control (Table 3), indicating participating effects of Pu in sustaining membrane integrity in plant cells (Yiu et al 2009).…”

Section: Callus Growth Traitsmentioning

confidence: 99%

“…To neutralize the effect of stresses caused through the elicitation process, the plant cells employ antioxidative activities in different ways such as enhancement of SMs (Golkar et al 2019;Nabi et al 2021). In this study, antioxidant activity was determined as DPPH radical scavenging activity.…”

Section: Dpph Activitymentioning

confidence: 99%

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WITHDRAWN: Optimization of Seed Germination, Callus Induction, and Secondary Metabolites Production in Allium Jesdianum: Elicitation by Methyl Jasmonate and Putrescine

Yazdanian

Golkar

Vahabi

et al. 2021

Preprint

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Allium jesdianum is considered as the most important genera of the Liliaceae family with various pharmacological properties. The present study was carried out to determine the best pre-germination treatment in A. jesdianum. The highest seed germination (58.33%) was observed at chemical treatment including the combination of α-naphthaleneacetic acid (1 mgL− 1) and benzyl aminopurine (3 mgL− 1) on Murashige and Skoog (MS) media. The highest callus induction (86.7%) was observed at MS/2 media, which was supplemented by NAA (1 mgL− 1) and BAP (3 mgL− 1) from hypocotyl explants. Two elicitors including methyl jasmonate (MeJ) (0, 25, 50, and 100 µM) and putrescine (Pu) (0, 0.5, and 1 mM) were used to investigate their effects on lipid peroxidation, callus growth traits, total phenolics content (TPC), the contents of total flavonoids (TFD), total flavonols (TFL), anthocyanin, total chlorophyll, total carotenoids and on antioxidant activity through DPPH (1,1-diphenyl-2-picrylhydrazyl) method under callus culture. The results showed the superiority of MeJ over Pu for increasing the secondary metabolites and antioxidant activity in callused of A. Jesdianum, compared to the control. The highest TPC (6.02 mg GAEg− 1 FW), TFD (0.52 mg QEg− 1 FW), and TFL (0.39 mg QEg− 1 FW) were observed under 50 µM of MeJ. Meanwhile, the highest value for anthocyanin (8.99 µ mol g− 1 FW) was achieved at 25 µM of MeJ. The highest DPPH activities were observed at 50 and 100 µM of MeJ. Putrescine (0.5 mM) elicitation showed only superiority for callus growth rate (0.53 mm day− 1) and carotenoids (0.045 mg g− 1 DW). In this study, effective seed germination and elicitation of A. Jesdianum callus cultures were developed for the first time. Thus, new protocols could be suitable for future studies in biotechnological aspects of this medicinal plant.

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Section: Callus Growth Traitsmentioning

confidence: 99%

Section: Callus Growth Traitsmentioning

confidence: 99%

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WITHDRAWN: Optimization of Seed Germination, Callus Induction, and Secondary Metabolites Production in Allium Jesdianum: Elicitation by Methyl Jasmonate and Putrescine

Yazdanian

Golkar

Vahabi

et al. 2021

Preprint

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“…El ácido salicílico y el quitosano se utilizan juntos para inducir la resistencia de la planta a los patógenos. Ambos ofrecen una alternativa al uso de fungicidas porque modulan la RSA y estimulan la producción de fenoles, terpenos y flavonoides en respuesta a la infección causada por hongos como Fusarium (Golkar et al, 2019). Por tanto, los objetivos del presente estudio fueron against pathogens.…”

Section: Fully Bilingualunclassified

“…Por tanto, los objetivos del presente estudio fueron against pathogens. They provide an alternative to fungicides by modulating SAR and by stimulating the production of phenols, terpenes and flavonoids in response to infection by fungi such as Fusarium (Golkar et al, 2019). Therefore, the objectives of this study were to evaluate the production of secondary metabolites of chitosan-treated plant extracts of P. auritum and to determine their in vitro fungicidal activity against F. oxysporum f. sp.…”

Section: Fully Bilingualmentioning

confidence: 99%

Chitosan-induced production of secondary metabolites in plant extracts of Piper auritum, and the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae

Fernández

Hernández-Ochoa

Carmona-Hernández

et al. 2020

RMF

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Se probó el efecto del quitosano sobre la producción de metabolitos secundarios de extractos etanólicos de Piper auritum, así como la actividad fungicida in vitro contra Fusarium oxysporum f. sp. vanillae. Las plantas de P. auritum se dividieron en seis parcelas y se añadió quitosano comercial a la mitad de ellas. Se midieron las concentraciones de flavonoides, fenoles, terpenos, alcaloides y ácido salicílico en extractos etanólicos de P. auritum y la actividad antifúngica se midió con la concentración efectiva media (CE50). Las concentraciones totales de flavonoides, fenoles y terpenos fueron más altas con el tratamiento con quitosano (sin quitosano 12.8 versus con quitosano 12.4 ?g equivalente de quercetina por mg, 12.6 versus 2.3 ?g equivalente de ácido tánico por 10 mg y 16.3 versus 11.6 mg equivalente de mentol por 100 mg). Mientras que la concentración de alcaloides disminuyó mediante la adición de quitosano (de 148.2 a 84.5 ?g de equivalente de piperina por mg). La adición de quitosano aumentó la concentración de ácido salicílico (de 1.3 a 2.2 ?g de equivalente de ácido salicílico por mg). La concentración de 4 mg mL-1 de extracto etanólico de P. auritum tratado con quitosano inhibió el 100% del crecimiento micelial. El tratamiento con quitosano generó que la CE50 de P. auritum contra F. oxysporum f. sp. vanillae fuera menor (1.5 mg mL-1) respecto al control (5.1 mg mL-1). Se concluye que la adición de quitosano aumentó la producción de metabolitos secundarios y la actividad antifúngica in vitro en extractos de P. auritum.

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Regulation of Plant Secondary Metabolism by Salicylic Acid Under Abiotic Stress

Lajayer¹,

Abbasi²,

Moghiseh³

et al. 2022

Managing Plant Stress Using Salicylic Acid

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The effects of chitosan and salicylic acid on elicitation of secondary metabolites and antioxidant activity of safflower under in vitro salinity stress

Cited by 80 publications

References 40 publications

WITHDRAWN: Optimization of Seed Germination, Callus Induction, and Secondary Metabolites Production in Allium Jesdianum: Elicitation by Methyl Jasmonate and Putrescine

WITHDRAWN: Optimization of Seed Germination, Callus Induction, and Secondary Metabolites Production in Allium Jesdianum: Elicitation by Methyl Jasmonate and Putrescine

Chitosan-induced production of secondary metabolites in plant extracts of Piper auritum, and the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae

Regulation of Plant Secondary Metabolism by Salicylic Acid Under Abiotic Stress

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