The effects of antioxidants on a porcine model of liver hemorrhage

Orfanos, Nikolaos; Mylonas, Anastasios; Karmaniolou, Iosifina; Stergiou, Irene P.; Lolis, Evangelos; Dimas, Cleanthi; Papalois, Αpostolos; Kondi-Pafiti, Agathi; Smyrniotis, Vasileios; Arkadopoulos, Nikolaos

doi:10.1097/ta.0000000000001026

Cited by 9 publications

(9 citation statements)

References 22 publications

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“…Jolly and co-workers have demonstrated a comparable effect in the myocardium [8]. Other studies carried out on the effect of antioxidant therapy following hemorrhagic shock on the liver [9], [10] and overall survival [11] have shown similar results. There is also a growing body of experimental evidence that implicates free radical dependent lipid peroxidation in injury to the central nervous system (CNS), as well as the myocardium [12], [13].…”

Section: Significancementioning

confidence: 69%

Significance of oxygen free radicals in the pathophysiology of hemorrhagic shock - A protocol

Premaratne

Amaratunga

Mensah

et al. 2018

International Journal of Surgery Protocols

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Section: Significancementioning

confidence: 69%

Significance of oxygen free radicals in the pathophysiology of hemorrhagic shock - A protocol

Premaratne

Amaratunga

Mensah

et al. 2018

International Journal of Surgery Protocols

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“…A pre-medication injection with midazolam (0.5 mg/kg) and ketamine 15 mg/kg was administered intramuscularly (IM). Atropine (0.045 mg/kg) was administered IM in the neck at 10 min prior to intubation (22)(23)(24)(25)(26). Two polyethylene intravenous catheters (18G) were inserted into two peripheral veins in both ears for infusion of crystalloids and anesthetic drugs.…”

Section: Methodsmentioning

confidence: 99%

“…General anesthesia was maintained with continuous infusion of propofol at 6-8 mg/kg/h, as well as fentanyl (0.012 mg/kg; Janssen-Cilag International NV; Beerse, Belgium) and cisatracurium (0.5 mg/kg; GlaxoSmithKline Manufacturing SpA, Verona, Italy) and the animals were mechanically ventilated. The femoral vein was also catheterized for blood collection (23,25,26). During the experiment, the animals were continuously monitored by electrocardiography, pulse oximetry and measurement of arterial blood pressure.…”

Section: Methodsmentioning

confidence: 99%

Lazaroid U‑74389G in liver ischemia‑reperfusion injury: A swine model

et al. 2019

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Reactive oxygen species have a key role in liver ischemia-reperfusion (I/R) injury. In the present study, the effect of the anti-oxidant compound lazaroid U-74389G in preventing liver I/R injury was investigated in a swine model. Ischemia was produced by portal vein occlusion. Two sets of experiments were performed, each with two groups (n=7 per group). In the first group, the potential protective effect of an intracaval injection of U-74389G after a 30-min ischemia, followed by a 60-min reperfusion period was assessed (biopsies at 0, 15, 30 and 90 min experimental time). In the second set, the effect of intracaval U-74389G injection after 30 min of ischemia, followed by a longer reperfusion period of 120 min was determined (biopsies at 0, 15, 30 and 150 min experimental time). Liver malondialdehyde, hepatocyte vacuolation-degeneration, venous congestion, inflammatory cell infiltration, sinus congestion-dilation and Chiu score of intestinal damage were determined at up to 150 min of reperfusion. In the second set of experiments, the Chiu score of intestinal damage was improved by the administration of U-74389G (3.17±0.40 vs. 4.33±0.21; P=0.030). However, in the two sets of experiments, the liver inflammatory reaction was more pronounced in the U-74389G groups (P=0.017 for the first set, P= 0.021 for the second set). No significant effect of U-74389G on any other parameters was detected. In conclusion, intestinal damage due to portal venous congestion

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“…Caspase expression as mentioned previously is induced with burn and HS and is often quantified to demonstrate activation of cell apoptosis [123,125,126]. In trauma and HS rats the amount of TUNEL positive cells in the kidney paralleled that of relative caspase 3 and 9…”

Section: Accepted Manuscriptmentioning

confidence: 98%

Molecular mechanisms of trauma-induced acute kidney injury: Inflammatory and metabolic insights from animal models

Burmeister

Gómez

Dubick

2017

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Trauma-induced acute kidney injury (AKI), such as after hemorrhagic shock (HS) or burn, remains a significant problem in the intensive care unit and is associated with increased mortality. The pathophysiology that drives AKI post-trauma is multi-factorial, and includes both inflammatory and metabolic alterations. Identifying the systemic profile that contributes to AKI is crucial not only for early diagnosis, but also for identifying treatments that improve kidney function and maintaining long-term patient health. In an effort to elucidate this molecular pathophysiology researchers have utilized a variety of animal models including chemically-induced (i.e., cisplatin), blocking renal perfusion (i.e., arterial clamping) and inducing burn or HS. As the latter burn and HS models are unequivocally applicable to studying AKI in the context of traumatic injury, this review will summarize the inflammatory and metabolic insights associated with AKI gained with these animal models. Moreover, novel therapeutic strategies brought forth with these models will be discussed.

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The effects of antioxidants on a porcine model of liver hemorrhage

Abstract: Our experimental study showed that coadministration of NAC and DFO during liver hemorrhage can decrease the amounts of fluids needed for resuscitation. Moreover, the antioxidant combination restores the energy dependent apoptosis and proliferation of the hepatocytes.

Cited by 9 publications

References 22 publications

Significance of oxygen free radicals in the pathophysiology of hemorrhagic shock - A protocol

Significance of oxygen free radicals in the pathophysiology of hemorrhagic shock - A protocol

Lazaroid U‑74389G in liver ischemia‑reperfusion injury: A swine model

Molecular mechanisms of trauma-induced acute kidney injury: Inflammatory and metabolic insights from animal models

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