The effect of two-layer (university of Wisconsin solution/perfluorochemical) preservation method on clinical grade pancreata prior to islet isolation and transplantation

Matsumoto, Shuichiro; Zhang, G; Qualley, Sabrina A.; Clever, Jared L.; Tombrello, Y; Strong, Douglas M.; Reems, Jo Anna

doi:10.1016/j.transproceed.2004.04.012

Cited by 17 publications

(21 citation statements)

References 11 publications

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“…Significant research effort has focused on investigating the efficacy of the two-layer method (TLM), the present state of the art for pancreas preservation. [3][4][5][6][7][8] In the late 1990s, many centers reported improvements in islet isolation outcome using TLM for CP. These improvements were attributed in part to increases in tissue ATP associated with enhanced tissue oxygenation with the TLM compared to the previously used CP in University of Wisconsin (UW) solution alone.…”

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confidence: 99%

Real-Time Noninvasive Assessment of Pancreatic ATP Levels During Cold Preservation

Scott

Matsumoto

Tanaka

et al. 2008

Transplantation Proceedings

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31P-NMR spectroscopy was utilized to investigate rat and porcine pancreatic ATP:P(i) ratios to assess the efficacy of existing protocols for cold preservation (CP) in maintaining organ quality. Following sacrifice, rat pancreata were immediately excised or left enclosed in the body for 15 minutes of warm ischemia (WI). After excision, rat pancreata were stored at 6 degrees C to 8 degrees C using histidine-tryptophan-ketoglutarate solution (HTK) presaturated with air (S1), HTK presaturated with O2 (S2), or the HTK/perfluorodecalin two-layer method (TLM) with both liquids presaturated with O2 (S3). 31P-NMR spectra were sequentially collected at 3, 6, 9, 12, and 24 hours of CP from pancreata stored with each of the three protocols examined. The ATP:Pi ratio for rat pancreata exposed to 15 minutes of WI and stored with S3 increased during the first 9 hours of CP, approaching values observed for organs procured with no WI. A marked reduction in the ATP:Pi ratio was observed beyond 12 hours of CP with S3. After 6 hours of CP, the ATP:Pi ratio was highest for S3, substantially decreased for S2, and below detection for S1. In sharp contrast to the rat model, ATP was barely detectable in porcine pancreata exposed to minimal warm ischemia (<15 minutes) stored with the TLM regardless of CP time. We conclude that 31P-NMR spectroscopy is a powerful tool that can be used to (1) noninvasively evaluate pancreata prior to islet isolation, (2) assess the efficacy of different preservation protocols, (3) precisely define the timing of reversible versus irreversible damage, and (4) assess whether intervention will extend this timing.

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confidence: 99%

Real-Time Noninvasive Assessment of Pancreatic ATP Levels During Cold Preservation

Scott

Matsumoto

Tanaka

et al. 2008

Transplantation Proceedings

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“…noting that reports of isolated human islet cultured in vitro showed a similar 2-4 fold stimulation of insulin secretion when grown in high glucose conditions Matsumoto et al, 2004).…”

Section: Discussionmentioning

confidence: 86%

“…The genetic basis for the glucose responsiveness in engineered cells is the vector, in which the TGFα promoter that is upregulated in high glucose conditions leads to increased expression of the down stream gene (McClain et al, 1992;Matsumoto et al, 2004). Having determined that the cell lines produced insulin in response to glucose, we asked if production of mature insulin in the engineered cells was uniquely dependent on furin activity, which is produced in glucoseregulated manner and ultimately is responsible for production of mature insulin.…”

Section: Production Of Mature Insulin Is Dependent On Proteolytic Actmentioning

confidence: 99%

Glucose-regulated insulin production from genetically engineered human non-beta cells

et al. 2007

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“…The two-layer preservation method was another factor favouring higher islet yields and better transplantation results. It has been demonstrated that the two-layer technique oxygenates and activates the metabolism of the pancreas, leading to resuscitation of pancreases with extended preservation time and/or ischaemically damaged tissue [11,24]. This might explain the higher islet counts and the improved success rate of transplantation compared with the UW preservation method, although the two-layer technique was performed in only eight cases and cold ischaemia times were uniformly short.…”

Section: Discussionmentioning

confidence: 99%

Pancreatic islet isolation variables in non-human primates (rhesus macaques)

et al. 2008

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The effect of two-layer (university of Wisconsin solution/perfluorochemical) preservation method on clinical grade pancreata prior to islet isolation and transplantation

Cited by 17 publications

References 11 publications

Real-Time Noninvasive Assessment of Pancreatic ATP Levels During Cold Preservation

Real-Time Noninvasive Assessment of Pancreatic ATP Levels During Cold Preservation

Glucose-regulated insulin production from genetically engineered human non-beta cells

Pancreatic islet isolation variables in non-human primates (rhesus macaques)

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