The effect of topographic characteristics on cell migration velocity

Kaiser, Jean‐Pierre; Reinmann, Andreas; Bruinink, A.

doi:10.1016/j.biomaterials.2006.06.002

Cited by 150 publications

(119 citation statements)

References 30 publications

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“…Nevertheless, some aspects deserve to be discussed. First, our results on the cell polarisation on lines and pillars are consistent with the results reported on microgroove topography [34] and on columnar microstructures fabricated by polymer demixing [35] or on three-dimensional sharp-tip microtopography [13]. In each case a clear relationship between the morphological parameters of normal cells and the substrate topography appears (e.g.…”

Section: Discussionsupporting

confidence: 90%

The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure

Tzvetkova-Chevolleau¹,

Stéphanou²,

Fuard³

et al. 2008

Biomaterials

160

110

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Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture and rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells of similar origin and morphology, but exhibiting a normal or cancerous phenotype. Using micropatterned polydimethylsiloxane substrates (PDMS) with tuneable stiffness (500kPa, 750kPa, 2000kPa) and topography (lines, pillars or unpatterned), we systematically analyse the differential response of normal (3T3) and cancer (SaI/N) fibroblastic cells. Our results demonstrate that both cells exhibit differential morphology and motility responses to changes in substrate rigidiy and microtopography. 3T3 polarization and spreading are influenced by substrate microtopography and rigidity. The cells exhibit a persistent type of migration, which depends on the substrate anisotropy. In contrast, the dynamic of SaI/N spreading is strongly modified by the substrate topography but not by substrate rigidity. SaI/N morphology and migration seem to escape from extracellular cues: the cells exhibit uncorrelated migration trajectories and a large dispersion of their migration speed, which increases with substrate rigidity. Abstract

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Section: Discussionsupporting

confidence: 90%

The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure

Tzvetkova-Chevolleau¹,

Stéphanou²,

Fuard³

et al. 2008

Biomaterials

160

110

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“…The resulting collective migration forms a multicellular spherical acinus or tube inside a structural constraint with a curved surface. 63,64,119 Control of cell migration through actin modulation has been well characterized based on contact guidance by means of simple synthetic 2.5D microtopographical features, such as parallel ridges and grooves, 73,96,[120][121][122][123][124] arrays of micropillars, 14,22,80,81 and asymmetric microchannels (Fig. 5B).…”

Section: Cell Migrationmentioning

confidence: 99%

“…125,126 In cells on a micro-/nano-grooved structure, actin filaments and vinculinrich focal adhesions align with the longer axis of the grooves. This effect causes various cells, such as epithelial, endothelial, glial cells, fibroblasts, osteoblasts, and neutrophils, to align 73,96,120,121 and migrate 4,121,123,124 along the grooves. On an array of micropillars, cellular protrusions are guided preferentially to the pillars.…”

Section: Cell Migrationmentioning

confidence: 99%

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Miyoshi

Adachi

2014

Tissue Engineering Part B: Reviews

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“…Cell behavior, such as cell adhesion, proliferation and gene expression, can be directed by the substrate geometry and surface chemistry (Clark et al 1990;Curtis and Wilkinson 1997;den Braber et al 1995;Dunn and Heath 1976;Kaiser et al 2006;Meyle et al 1994;Rebollar et al 2008;Rovensky et al 1999;Rovensky and Samoilov 1994;Suh et al 2004; Thomas et al 1999Thomas et al , 2002. Various studies on these effects have been reported, most utilizing substrates bearing nano-scale (Rebollar et al 2008) to micro-scale (Clark et al 1990;Curtis and Wilkinson 1997;den Braber et al 1995;Kaiser et al 2006;Meyle et al 1994) patterned surfaces as well as chemically defined surfaces (Suh et al 2004;Thomas et al 1999Thomas et al , 2002. In addition to the patterned flat surface, cylindrical substrates with high degree of curvature have been shown to affect morphogenetic response of cultured cells (Dunn and Heath 1976;Rovensky et al 1999;Rovensky and Samoilov 1994).…”

Section: Introductionmentioning

confidence: 99%

Self-standing aligned fiber scaffold fabrication by two photon photopolymerization

Hidai

Jeon

Hwang

et al. 2009

Biomed Microdevices

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Development of materials and fabrication techniques lead the growth of three-dimensional cell culture matrices in biomedical engineering. In this work, we present a method for fabricating self-standing fiber scaffolds by two-photon polymerization induced by a femtosecond laser. The aligned fibers are 330 μm long with a diameter of 6-9 μm. Depending on the pitch of the aligned fibers, various cell morphologies are distinguished via three-dimensional images. Furthermore, the morphologies of fibroblast cells (NIH-3T3) and epithelial cells (MDCK) on the fiber scaffolds are studied to show the effect of high curvature (3-4.5 μm radii) on cell morphology. NIH-3T3 cells that contain straight pattern of actin microfilament bundles are extended and partly wrap single fibers or tend to reside between fibers. On the other hand, MDCK cells that contain circular pattern of actin microfilament bundles cover the fiber peripheral surface exhibiting high aspect ratio elongation. These results indicate that cell morphology on fiber scaffolds is influenced by the pattern of actin microfilament bundles.

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The effect of topographic characteristics on cell migration velocity

Cited by 150 publications

References 30 publications

The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure

The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Self-standing aligned fiber scaffold fabrication by two photon photopolymerization

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