The Effect of the Taste Receptor Protein T1R3 on the Development of Islet Tissue of the Murine Pancreas

Муровец, В. О.; Sozontov, Egor A.; Zachepilo, T. G.

doi:10.1134/s0012496619010010

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…Crem knockout mice exhibit similar morphological characteristics to our mice fed on high-dose sodium saccharin, while protamine 1 ( Prm1 ), transition protein 1 ( Tnp1 ) and other CREM-regulated genes are down-regulated in Tas1r3 / Gnat three double knockout mice ( Blendy et al, 1996 ; Nantel et al, 1996 ; Mosinger et al, 2013 ; Gong et al, 2016a ). Besides, TATA and TATA-box binding protein (TBP) activates the spermatogenesis process ( Jing et al, 2016 ; Wu et al, 2016 ; Murovets et al, 2019 ). These reports provide insights to explore the mechanism of spermatogenesis further.…”

Section: Introductionmentioning

confidence: 99%

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Taste receptors affect male reproduction by influencing steroid synthesis

Liu

Gong

Shi

et al. 2022

Front. Cell Dev. Biol.

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For the male genetic materials to reach and fertilize the egg, spermatozoa must contend with numerous environmental changes in a complex and highly sophisticated process from generation in the testis, and maturation in the epididymis to capacitation and fertilization. Taste is an ancient chemical sense that has an essential role in the animal’s response to carbohydrates in the external environment and is involved in the body’s energy perception. In recent years, numerous studies have confirmed that taste signaling factors (taste receptor families 1, 2 and their downstream molecules, Gα and PLCβ2) are distributed in testes and epididymis tissues outside the oral cavity. Their functions are directly linked to spermatogenesis, maturation, and fertilization, which are potential targets for regulating male reproduction. However, the specific signaling mechanisms of the taste receptors during these processes remain unknown. Herein, we review published literature and experimental results from our group to establish the underlying signaling mechanism in which the taste receptor factors influence testosterone synthesis in the male reproduction.

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Section: Introductionmentioning

confidence: 99%

“…For example, the cytosolic Ca 2 ⁺ and cAMP levels in Tas1r1 -deficient sperm are significantly elevated ( Meyer et al, 2012 ). In addition, the expression of Tas1r3 is associated with regulating insulin secretion through apoptosis ( Murovets et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Taste receptors affect male reproduction by influencing steroid synthesis

Liu

Gong

Shi

et al. 2022

Front. Cell Dev. Biol.

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“…Obesity, which is caused by long-term high-calorie consumption, can increase anxiety through insulin or leptin resistance, systemic inflammation, and hormonal dysregulation [ 36 ]. TAS1R3, which is expressed in metabolic tissues such as adipocytes and pancreatic β cells, has been reported to regulate metabolism [ 37 , 38 ]. Studies on mice with genetic ablation of TAS1R3 revealed differences in weight gain compared to WT mice [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Taste receptor type 1 member 3 enables western diet-induced anxiety in mice

Song,

Lee,

Seong

et al. 2023

BMC Biol

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Background Accumulating evidence supports that the Western diet (WD), a diet high in saturated fat and sugary drinks, contributes to the pathogenesis of anxiety disorders, which are the most prevalent mental disorders worldwide. However, the underlying mechanisms by which WD causes anxiety remain unclear. Abundant expression of taste receptor type 1 member 3 (TAS1R3) has been identified in the hypothalamus, a key brain area involved in sensing peripheral nutritional signals and regulating anxiety. Thus, we investigated the influence of excessive WD intake on anxiety and mechanisms by which WD intake affects anxiety development using wild-type (WT) and Tas1r3 deficient (Tas1r3−/−) mice fed a normal diet (ND) or WD for 12 weeks. Results WD increased anxiety in male WT mice, whereas male Tas1r3−/− mice were protected from WD-induced anxiety, as assessed by open field (OF), elevated plus maze (EPM), light–dark box (LDB), and novelty-suppressed feeding (NSF) tests. Analyzing the hypothalamic transcriptome of WD-fed WT and Tas1r3−/− mice, we found 1,432 genes significantly up- or down-regulated as a result of Tas1r3 deficiency. Furthermore, bioinformatic analysis revealed that the CREB/BDNF signaling-mediated maintenance of neuronal regeneration, which can prevent anxiety development, was enhanced in WD-fed Tas1r3−/− mice compared with WD-fed WT mice. Additionally, in vitro studies further confirmed that Tas1r3 knockdown prevents the suppression of Creb1 and of CREB-mediated BDNF expression caused by high levels of glucose, fructose, and palmitic acid in hypothalamic neuronal cells. Conclusions Our results imply that TAS1R3 may play a key role in WD-induced alterations in hypothalamic functions, and that inhibition of TAS1R3 overactivation in the hypothalamus could offer therapeutic targets to alleviate the effects of WD on anxiety.

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“…T1R3-knockout animals are resistant to sucrose-induced obesity and have smaller fat depots when fed a high-sucrose diet [15]. Deletion of Tas1r3-/-reduces activation of mTORC1 [14] and decreases density of pancreatic islets and expression of caspase 3 [18].…”

Section: Introductionmentioning

confidence: 99%

Allelic variation of the Tas1r3 taste receptor gene affects sweet taste responsiveness and metabolism of glucose in F1 mouse hybrids

et al. 2020

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In mammals, inter-and intraspecies differences in consumption of sweeteners largely depend on allelic variation of the Tas1r3 gene (locus Sac) encoding the T1R3 protein, a sweet taste receptor subunit. To assess the influence of Tas1r3 polymorphisms on feeding behavior and metabolism, we examined the phenotype of F 1 male hybrids obtained from crosses between the following inbred mouse strains: females from 129SvPasCrl (129S2) bearing the recessive Tas1r3 allele and males from either C57BL/6J (B6), carrying the dominant allele, or the Tas1r3-gene knockout strain C57BL/6J-Tas1r3 tm1Rfm (B6-Tas1r3-/-). The hybrids 129S2B6F1 and 129S2B6-Tas1r3-/-F1 had identical background genotypes and different sets of Tas1r3 alleles. The effect of Tas1r3 hemizygosity was analyzed by comparing the parental strain B6 (Tas1r3 homozygote) and hemizygous F 1 hybrids B6 × B6-Tas1r3-/-. Data showed that, in 129S2B6-Tas1r3-/-F1 hybrids, the reduction of glucose tolerance, along with lower consumption of and lower preference for sweeteners during the initial licking responses, is due to expression of the recessive Tas1r3 allele. Hemizygosity of Tas1r3 did not influence these behavioral and metabolic traits. However, the loss of the functional Tas1r3 allele was associated with a small decline in the long-term intake and preference for sweeteners and reduction of plasma insulin and body, liver, and fat mass.

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The Effect of the Taste Receptor Protein T1R3 on the Development of Islet Tissue of the Murine Pancreas

Cited by 7 publications

References 13 publications

Taste receptors affect male reproduction by influencing steroid synthesis

Taste receptors affect male reproduction by influencing steroid synthesis

Taste receptor type 1 member 3 enables western diet-induced anxiety in mice

Allelic variation of the Tas1r3 taste receptor gene affects sweet taste responsiveness and metabolism of glucose in F1 mouse hybrids

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