The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impacton the coagulant function of stored platelets

Shapira, Shirley; Friedman, Ziva; Shapiro, Hava; Presseizen, Katya; Radnay, Judith; Ellis, Martin

doi:10.1046/j.1537-2995.2000.40101257.x

Cited by 59 publications

(55 citation statements)

References 30 publications

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“…Possible effectors of the immunosuppressive side effects of PLT transfusions are the large amounts of PLT ectosomes (PLT-Ecto) that are released during storage and transfused concomitantly with PLT (18,19). Placke et al (20) showed that PLT shed and transfer their MHC class I molecules to tumor cells, conferring them resistance to NK cells.…”

mentioning

confidence: 99%

Platelet-Derived Ectosomes Reduce NK Cell Function

Sadallah

Schmied

Eken

et al. 2016

The Journal of Immunology

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Platelet (PLT) transfusions are potentially life saving for individuals with low PLT numbers; however, previous work revealed that PLT transfusions are associated with increased infection risk. During storage, PLT intended for transfusion continuously shed ectosomes (Ecto) from their surface, which express immunomodulatory molecules like phosphatidylserine or TGF-β1. Recently, PLT-Ecto were shown to reduce proinflammatory cytokine release by macrophages and to favor the differentiation of naive T cells toward regulatory T cells. Whether PLT-Ecto modify NK cells remains unclear. We exposed purified NK cells and full PBMCs from healthy donors to PLT-Ecto. We found a reduced expression of several activating surface receptors (NKG2D, NKp30, and DNAM-1) and decreased NK cell function, as measured by CD107a expression and IFN-γ production. Pretreatment of PLT-Ecto with anti–TGF-β1 neutralizing Ab restored surface receptor expression and NK cell function. We further observed a TGF-β1–mediated upregulation of miR-183, which, in turn, reduced DAP12, an important protein for stabilization and downstream signaling of several activating NK cell receptors. Again, these effects could antagonized, in part, when PLT-Ecto were preincubated with anti–TGF-β1 Ab. Erythrocyte Ecto did not affect NK cells. Polymorphonuclear cell Ecto expressed MHC class I and inhibited NK cell function. In addition, they induced the secretion of TGF-β1 by NK cells, which participated in an auto/paracrine manner in the suppressive activity of polymorphonuclear cell–derived Ecto. In sum, our study showed that PLT-Ecto could inhibit NK cell effector function in a TGF-β1–dependent manner, suggesting that recipients of PLT transfusions may experience reduced NK cell function.

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confidence: 99%

Platelet-Derived Ectosomes Reduce NK Cell Function

Sadallah

Schmied

Eken

et al. 2016

The Journal of Immunology

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“…a potential haemostatic advantage of stored red cell and platelet units may be explained by the observation that during storage red cells and platelets showed a progressive increase in membrane phosphtidylserine expression that correlates with increased thrombin generation [17]. Furthermore, platelet storage is associated with microparticle formation that has a potent effect on thrombus generation and propagation [18].…”

Section: Discussionmentioning

confidence: 99%

Storage of Blood Components Does Not Decrease Haemostatic Potential: In vitro Assessment of Fresh versus Stored Blood Components Using Thromboelastography

Bartfeld

Ellis²,

Lubetzky³

et al. 2010

Transfus Med Hemother

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Background: Major surgery and severe trauma typically lead to massive blood loss requiring rapid transfusion of large amounts of blood products. It has been suggested that fresh, unrefrigerated whole blood provides a haemostatic advantage in this setting. The aim of the current study was to compare the clot formation parameters of fresh, unrefrigerated whole blood and whole blood reconstituted from components stored for varying periods of time, using rotational thromboelastography (ROTEM®). Methods: Fresh whole blood and reconstituted whole blood using combinations of nonleucoreduced red cell units (stored for 7, 14, 21, 28, or 35 days), platelet concentrates (stored for 1, 3 or 5 days), and fresh frozen plasma (stored for 6 months) were analysed using ROTEM. Measurements of the clotting time (CT), clot formation time (CFT), and maximal clot firmness (MCF) were compared between units of fresh whole blood and reconstituted whole blood samples. Results: There was no difference in the haemostatic parameters measured of fresh whole blood and reconstituted whole blood using red cell units stored for less than 21 days. ROTEM demonstrated that the CT and CFT were significantly shorter for reconstituted whole blood samples using red cells stored for longer than 21 days when compared to fresh whole blood and to reconstituted whole blood samples using red cell units stored for less than 21 days. The CT was inversely correlated to the duration of platelet storage. The MCF was unchanged regardless of duration of blood product storage. Conclusion: Fresh unrefrigerated whole blood and blood products stored for short duration (less than 21 days) were not superior to those stored for longer durations.

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“…But this platelet staining method needs to perform an incubation of the dye with platelet in vitro. The routinely used procedures to prepare platelet-rich plasma are based on centrifugation (16)(17)(18). It has been recently demonstrated that such procedures lead to changes of platelets characteristics and specially concerning the expression of surface markers.…”

Section: µMmentioning

confidence: 99%

<title>Intravital fluorescence microscopic study of the behavior of long-circulating liposomes during microvascular thrombosis</title>

et al. 2002

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Treatment of thrombosis depends on the selectivity of thrombolytic agents to the clot. It has been already demonstrated that liposomes can provide a better selectivity of such agents to the clot site. We have recently shown that intravital fluorescence microscopy is a powerful tool to image in situ and in real time the labelling of leukocytes by long circulating liposomes. The aim of this study was to monitor the in vivo behaviour of such liposomes in a clot site. Carboxyfluorescein-loaded long circulating liposomes were prepared and characterized in term of size and permeability. The liposomes suspension was injected intravenously to golden hamsters. The skin microcirculation was observed using a dorsal skin-fold chamber by fluorescence microscopy. Thrombosis were obtained as the consequence of the inflammatory response due to the surgery. Using this model, fluorescent dots were observed at the site of the clot. Liposomes accumulate at the clot site whatever the mechanism (passive deposition or uptake). There is a period of latency and 30 seconds after the blood flow stop, fluorescence increases very rapidly and a bright fluorescent spot is observed at the site of the clot. Further studies are needed to determine the exact localization of liposomes in the clot and the mechanism of interaction.

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The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impacton the coagulant function of stored platelets

Cited by 59 publications

References 30 publications

Platelet-Derived Ectosomes Reduce NK Cell Function

Platelet-Derived Ectosomes Reduce NK Cell Function

Storage of Blood Components Does Not Decrease Haemostatic Potential: In vitro Assessment of Fresh versus Stored Blood Components Using Thromboelastography

<title>Intravital fluorescence microscopic study of the behavior of long-circulating liposomes during microvascular thrombosis</title>

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