The effect of some environmental factors on herpes virus grown in HeLa cells

Farnham, Ann E.; Newton, Alison A.

doi:10.1016/0042-6822(59)90073-x

Cited by 51 publications

(29 citation statements)

References 15 publications

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“…Inactivation of the virus remaining on the cell surface with acid was a preferred method to neutralization by antibody or light inactivation of photosensitized virus (Spivack et al, 1982;DeLuca et al, 1981) because it requires a minimum amount of time for handling, allows better evaluation of the kinetics of virus uptake and deals with only those events which are occurring at the cell surface. Penetration of the virus was synchronized by incubation with the cells at 4 °C, followed by removal of unbound virus prior to the temperature shift to 37 °C (Huang & Wagner, 1964;Farnham & Newton, 1959).…”

Section: Ph Dependence Of Hsv-1 Kos Penetrationmentioning

confidence: 99%

Mild Acidic pH Inhibition of the Major Pathway of Herpes Simplex Virus Entry into HEp-2 Cells

Rosenthal

Killius

Hodnichak

et al. 1989

Journal of General Virology

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SUMMARYPenetration of the KOS strain of herpes simplex virus type 1 (HSV-1) and the MS and 333 strains of herpes simplex virus type 2 (HSV-2) into HEp-2 cells at pH 6.3 was at least 100-fold less efficient than at pH 7-4. Penetration of two low passage clinical isolates was completely blocked at pH 6.3. The syncytium-forming HSV-1 strains GC and MP were less sensitive than KOS to the mild acidic conditions. The inhibition was completely reversed upon neutralization of the medium. Penetration was assayed by plaque production following protection from acid inactivation upon virus entry. Penetration of HSV-1 KOS into Vero and HEL diploid fibroblast cells was similarly inhibited. HSV-1 KOS grown in 2-deoxy-D-glucose and monensin was also extensively inhibited at pH 6.3 but virus grown in 2-deoxy-D-glucose penetrated more slowly than normal virus at pH 7.4. Electron microscopy of HSV-1 KOS infection indicated that fusion and endocytosis occur at both pH 7.4 and 6.3 but that fusion predominates at pH 7-4 and endocytosis predominates at pH 6.3. These results indicate that fusion at the plasma membrane is the major route of productive entry for HSV, that Strains of HSV can differ in their pH dependence for penetration and this may determine whether virus infection can occur following endocytic uptake.

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Section: Ph Dependence Of Hsv-1 Kos Penetrationmentioning

confidence: 99%

Mild Acidic pH Inhibition of the Major Pathway of Herpes Simplex Virus Entry into HEp-2 Cells

Rosenthal

Killius

Hodnichak

et al. 1989

Journal of General Virology

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“…The infectivity of HHV-6 was stable at pH values higher than 7.0 but was inactivated below pH 6.5 and the standard deviation of residual infectivity of three experiments at pH 6.5 at 37 °C was large compared with the others, which suggested that this pH might be the critical point for the inactivation of HHV-6. The stability of HHV-6 was similar to that of HSV (Farnham & Newton, 1959).…”

Section: Effect Of Ph On Hhv-6 Infectivitymentioning

confidence: 57%

“…HHV-6 infectivity was stable at physiological temperature and neutral pH as shown in Tables 2 and 3 and the thermal stability of HHV-6 was similar to those of HSV (Kaplan, 1957;Hoggan & Roizman, 1959) and HCMV (Wentworth & Gloyd, 1968) but different from those of HSV (Farnham & Newton, 1959) and HCMV (Vonka & Benyesh-Melnick, 1966). However, because the stability of the virus is influenced by the constituents of the diluent or storing buffers (Asano & Takahashi, 1978;Farnham & Newton, 1959;Vonka & BenyeshMelnick, 1966;Wentworth & Gloyd, 1968;Hoggan & Roizman, 1959), it is difficult to compare results on the thermal stability of viruses.…”

Section: Effect Of Ph On Hhv-6 Infectivitymentioning

confidence: 99%

“…However, because the stability of the virus is influenced by the constituents of the diluent or storing buffers (Asano & Takahashi, 1978;Farnham & Newton, 1959;Vonka & BenyeshMelnick, 1966;Wentworth & Gloyd, 1968;Hoggan & Roizman, 1959), it is difficult to compare results on the thermal stability of viruses. The infectivity of HHV-6 was stable at pH values higher than 7.0 but was inactivated below pH 6.5 and the standard deviation of residual infectivity of three experiments at pH 6.5 at 37 °C was large compared with the others, which suggested that this pH might be the critical point for the inactivation of HHV-6.…”

Section: Effect Of Ph On Hhv-6 Infectivitymentioning

confidence: 99%

“…Certain strains of HHV-6 grow well in certain cell lines and significant amounts of cell-free virus have been produced (Ablashi et al, 1987;Okuno et al, 1989;Shiraki et al, 1989a, b). The stability of infectivity is much influenced by the particular preparation and buffers used to contain celt-free virus (Asano & Takahashi, 1978;Farnham & Newton, 1959;Vonka & BenyeshMelnick, 1966;Wentworth & Gloyd, 1968;Hoggan & Roizman, 1959) and therefore the virus particle/infectivity ratio of HHV-6 will be much improved when appropriate conditions for the preparation of cell-free virus are achieved. The virus particle/infectivity ratio of the sonicated infected cells was higher than that of the culture supernatants, but this would not necessarily imply a low efficiency of infectivity of cell-free virus in sonicated infected cells.…”

Section: Effect Of Ph On Hhv-6 Infectivitymentioning

confidence: 99%

See 2 more Smart Citations

Physicochemical Characterization of Human Herpesvirus 6 Infectivity

Shiraki

Mukai²,

Okuno³

et al. 1991

Journal of General Virology

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The Effect of Herpes Simplex Virus Type 1 L-Particles on Virus Entry, Replication, and the Infectivity of Naked Herpesvirus DNA

Dargan

Subak‐Sharpe

1997

Virology

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Herpes simplex virus type 1(HSV-1) L-particles are known to be composed mainly of envelope and tegument proteins, to lack the nucleocapsid, and to be noninfectious. Thus L-particles represent interesting vaccine candidates. L-particles at > 1000/cell interfered with HSV-1 virion adsorption and penetration While L-particles did not affect HSV-1 growth kinetics in resting or nonresting BHK cultures infected with purified virions, treatment with L-particles before, or after, transfection with HSV-1 DNA resulted in a progressive increase in plaque numbers (five- to sixfold at 1000 L-particles/cell). Transfection assays using HSV-1 ts mutant DNA (ts 1201) revealed that enhancement was due to induction of otherwise nonreplicating genomes. The enhancement obtained with L-particles produced by WT HSV-1 or by mutants that are either deleted, or defective, in certain gene products was compared. Most important were the Vmw110 (ICP0) and Vmw65 (alpha-TIF) proteins, but VP11/12, VP13/14, and vhs also have a role. The L-particle-associated Vmw175 (ICP 4) protein did not appear be involved. The effect of homologous and heterologous combinations of pseudorabies virus, equineherpesvirus-1, and HSV-1 DNA's and L-particles was investigated in transfection assays. The L-particles of each virus, to varying extent, enhanced the plaquing efficiency of their own DNA but were also effective in heterologous combinations.

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The effect of some environmental factors on herpes virus grown in HeLa cells

Cited by 51 publications

References 15 publications

Mild Acidic pH Inhibition of the Major Pathway of Herpes Simplex Virus Entry into HEp-2 Cells

Mild Acidic pH Inhibition of the Major Pathway of Herpes Simplex Virus Entry into HEp-2 Cells

Physicochemical Characterization of Human Herpesvirus 6 Infectivity

The Effect of Herpes Simplex Virus Type 1 L-Particles on Virus Entry, Replication, and the Infectivity of Naked Herpesvirus DNA

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