The Effect of Semax and Its C-End Peptide PGP on the Morphology and Proliferative Activity of Rat Brain Cells During Experimental Ischemia: A Pilot Study

Stavchansky, Vasily V.; Yuzhakov, Vadim V.; Botsina, A. Yu.; Skvortsova, Veronika; Bondurko, Lyubov N.; Tsyganova, M.G.; Limborska, Svetlana A.; Myasoedov, N. F.; Dergunova, Lyudmila V.

doi:10.1007/s12031-010-9421-2

Cited by 30 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Three hours after occlusion, Semax altered the expression of genes associated with the artery vasodilation process as well. Our earlier studies showed that capillary bore extension was observed as early as 15 min after the administration of the peptide [9]. …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: genome-wide transcriptional analysis

et al. 2014

View full text Add to dashboard Cite

BackgroundThe nootropic neuroprotective peptide Semax (Met-Glu-His-Phe-Pro-Gly-Pro) has proved efficient in the therapy of brain stroke; however, the molecular mechanisms underlying its action remain obscure. Our genome-wide study was designed to investigate the response of the transcriptome of ischemized rat brain cortex tissues to the action of Semax in vivo.ResultsThe gene-expression alteration caused by the action of the peptide Semax was compared with the gene expression of the “ischemia” group animals at 3 and 24 h after permanent middle cerebral artery occlusion (pMCAO). The peptide predominantly enhanced the expression of genes related to the immune system. Three hours after pMCAO, Semax influenced the expression of some genes that affect the activity of immune cells, and, 24 h after pMCAO, the action of Semax on the immune response increased considerably. The genes implicated in this response represented over 50% of the total number of genes that exhibited Semax-induced altered expression. Among the immune-response genes, the expression of which was modulated by Semax, genes that encode immunoglobulins and chemokines formed the most notable groups.In response to Semax administration, 24 genes related to the vascular system exhibited altered expression 3 h after pMCAO, whereas 12 genes were changed 24 h after pMCAO. These genes are associated with such processes as the development and migration of endothelial tissue, the migration of smooth muscle cells, hematopoiesis, and vasculogenesis.ConclusionsSemax affects several biological processes involved in the function of various systems. The immune response is the process most markedly affected by the drug. Semax altered the expression of genes that modulate the amount and mobility of immune cells and enhanced the expression of genes that encode chemokines and immunoglobulins. In conditions of rat brain focal ischemia, Semax influenced the expression of genes that promote the formation and functioning of the vascular system.The immunomodulating effect of the peptide discovered in our research and its impact on the vascular system during ischemia are likely to be the key mechanisms underlying the neuroprotective effects of the peptide.

show abstract

Section: Discussionmentioning

confidence: 99%

“…We have previously shown the effect of Semax on the expression of genes that encode neurotrophic factors and their receptors in an experimental model ischemia in the rat brain [8,9]. …”

Section: Introductionmentioning

confidence: 99%

The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: genome-wide transcriptional analysis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…A significant increase in the expression of the nuclear protein PCNA, which participates in the preparation for cell division, was also noted. Judging by the upregulation of PCNA in the nuclei of the brain cells of control animals, Semax appears to stimulate the proliferation of repopulating elements of the nervous tissue [73].…”

Section: Neurotrophic Effectmentioning

confidence: 98%

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Kolomin¹,

Шадрина²,

Slominsky³

et al. 2013

View full text Add to dashboard Cite

Natural regulatory peptides are biologically active compounds that are produced by various cells and provide a link among the main regulatory systems of the body. The field of research into the biologic activity of endogenous regulatory peptides is extremely vast. These peptides affect the cardiovascular, immune, reproductive, endocrine, digestive, and other systems, alter energy metabolism, and are especially effective in the regulation of the central nervous system. Despite of the wide range of preventive and therapeutic effects of natural regulatory peptides and proteins, their application in clinical practice is difficult. This is primarily because of their extreme instability, as they are rapidly degraded by proteases of the gastrointestinal tract, blood, cerebrospinal fluid, and other biologic media. Compounds with higher stability (i.e., a considerably longer half-life compared with that of natural molecules) and the ability to provide a directional effect on the various body systems were obtained from modifications of endogenous regulatory peptides. Synthetic analogs of regulatory peptides, as a rule, contain only natural amino acids in their composition, and their biodegradation does not lead to the formation of toxic products; thus, they have fewer side effects. This review focuses on the consideration of two synthetic regulatory peptides, Semax and Selank, which were the bases for the creation of new drugs that are used effectively in the treatment of various diseases of the nervous system. The synthetic analog of an adrenocorticotropic hormone 4-10 fragment (ACTH 4-10) Semax is a powerful neuroprotective agent that is particularly effective as a therapy for stroke. Selank was synthesized on the basis of the natural immunomodulator tuftsin. Selank is a powerful anxiolytic that is used as a therapy for generalized anxiety disorder and neurasthenia without sedative and muscle-relaxant effects. This review presents the results of research aimed at studying the influence of these peptides on the transcriptome of brain cells. The problems of drugs developed based on the clinical activities of Semax and Selank are discussed separately.

show abstract

“…Пептид семакс (Met-Glu-His-Pro-Gly-Pro), синтезований в Інституті молекулярної генетики РАН, є нейроактивним аналогом адренокортикотропного гормону (АКТГ), який має низку нейропротекторних властивостей. У ЦНС він сприяє підвищенню рівня проліферації нейроглії і ендотелію кровоносних капілярів, а також поліпшенню стану нервової тканини при ішемії [1]. Семакс широко використовується при лікуванні алергічних і запальних захворювань зорового нерва [2], позитивно впливає на розвиток пам'яті, навчання і уваги.…”

Section: вступunclassified

Effect of Peptide Semax on Synaptic Activity and Short-Term Plasticity of Glutamatergic Synapses of Co-Cultured Dorsal Root Ganglion and Dorsal Horn Neurons

Shypshyna¹,

Veselovsky²,

Myasoedov³

et al. 2015

Fiziol Zh

View full text Add to dashboard Cite

The Effect of Semax and Its C-End Peptide PGP on the Morphology and Proliferative Activity of Rat Brain Cells During Experimental Ischemia: A Pilot Study

Cited by 30 publications

References 24 publications

The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: genome-wide transcriptional analysis

The peptide semax affects the expression of genes related to the immune and vascular systems in rat brain focal ischemia: genome-wide transcriptional analysis

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Effect of Peptide Semax on Synaptic Activity and Short-Term Plasticity of Glutamatergic Synapses of Co-Cultured Dorsal Root Ganglion and Dorsal Horn Neurons

Contact Info

Product

Resources

About