The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L. ‘Alba’

Abstract

Key message Tissue cultures are the only effective way to proliferate this valuable species (Daphne mezereum). These researches may help in solving the problem of reproduction of other woody plants. Abstract Daphne mezereum L. commonly known as February daphne or mezereon is a decorative shrub from the family Thymelaeaceae. It is a valuable nursery plant due to its high ornamental values, but its propagation by traditional methods is ineffective, therefore, little profitable. Micropropagation may be a good alternative way to produce this ornamental shrub. The experiment aimed to examine the effect of cytokinins (meta-Topolin, benzyladenine, and zeatin) and other growth regulators (gibberellic acid GA 3 and auxin 1-naphthalene acetic acid NAA) added to the Murashige and Skoog (MS) and Woody Plant Medium (WPM) nutrients on regeneration of explants of D. mezereum 'Alba'. In the all treatments containing plant growth regulators (PGR), 100% regeneration of explants has been observed. The highest number of shoots, after 6 weeks of culture, was produced on MS medium with 1 mg•L −1 BA and 0.1 mg•L −1 NAA. The longest shoots were produced on MS medium with 0.1 mg•L −1 of gibberellic acid (GA 3) and 1 mg•L −1 of meta-Topolin (mT). Generally, the type and concentration of plant growth regulators had an essential effect on regeneration and growth of shoots of Daphne mezereum 'Alba' in the in vitro culture.

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

“…Therefore, the use of appropriate concentration of auxin are important to control the growth and development of plants in vitro. This is confirmed by the result of research on Daphne mezereum L. ‘Alba’ where they reported optimum rooting formation using lower concentration of auxin [ 49 ]. The present study suggested that IAA at the concentration of 0.5-mg L −1 is the optimum to induce root.…”

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

“…Leaf samples from in vitro conditions (prior to transplanting) and 4-week-old acclimatized plants were collected for SEM examination. Following a fifteen-minute gradual dehydration with an increasing alcohol series (30,50,70,90, and 100%), the leaves were fixed in 2% (v/v) glutaraldehyde (Merck, Merck Specialities Pvt. Ltd., Mumbai, India) and left at room temperature for two hours.…”

Optimizing micropropagation conditions for a recalcitrant ninebark (Physocarpus opulifolius L. maxim.) cultivar

“…Cytokinins are adenine derivatives capable of inducing cell divisions in tissue cultures, initiating shoots and stimulating their proliferation and further growth, both of which are critical for in vitro multiplication efficiency. Exogenously applied cytokinins are more active in the presence of an auxin (Perveen et al 2011;Faisal et al 2018;Nowakowska et al 2019). Frequently used cytokinins are of natural origin such as zeatin (Zea), the 2-isopenthenyladenine (2iP), kinetin (Kin), and 6-benzyladenine (BA) or synthetic cytokinins such as thidiazuron (TDZ) (Pierik 1997).…”