The Effect of Rennin Upon Casein

Pertzoff, Vladimir

doi:10.1085/jgp.10.6.987

Cited by 6 publications

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“…In the preceding investigation upon the property of paracasein (1) we found that one of the six paracasein preparations studied, namely, Preparation VI, dissolved in NaOH at a rate of 1700 gin. per tool of base added.…”

Section: Paracasein Preparation VImentioning

confidence: 99%

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The Effect of Rennin Upon Casein

Pertzoff

1928

Journal of General Physiology

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In a preceding investigation (1) upon certain properties of paracasein, we attempted to identify paracasein by physicochemical means.Paracaseins were prepared by coagulating milk by means of rennin or pepsin preparations, puri/ying the resulting paracasein in the same way as was done in the case of casein (2). Such preparations, free from any proteolytic enzyme, were then tested for their solubility in water and for their solubility in dilute NaOH solutions at two temperatures. It was found that although paracasein at about 6°C. dissolves in water to the same extent as casein, the capacity to bind base was distinctly different from that of casein. While casein at 21 to 37°C. dissolves in combination with base to the extent of about 2100 gm. per tool of NaOH added (2, 3), most of the paracasein preparations combined with NaOH in this temperature range with a combining weight of 1450 gm.The ratio of the equivalent combining weight of casein to the combining weight of paracasein is as 1 to 1.45. Solubility measurements at 5°C. indicated that the same relationship held true for these proteins at low temperature.In the present investigation, we have studied further some of the other properties of paracasein preparations, among which are the hydrogen ion activity in systems composed of paracasein and base, and the maximum base-binding capacity of certain paracaseins.

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Section: Paracasein Preparation VImentioning

confidence: 99%

“…for 24 hours. The preparation was then precipitated and purified in the same way as has been already described (1). The suspension was then made to contain 30 rag.…”

Section: Paracasein Preparation VImentioning

confidence: 99%