The Effect of Regular Aerobic Exercise on Reverse Cholesterol Transport A1 and Apo Lipoprotein A-I Gene Expression in Inactive Women

Abstract

Background:Atherosclerotic cardiovascular disease is currently a cause of mortality in some parts of the world. The ATP-Binding Cassette Transporter (ABCA1) gene prepares instructions to produce the ATP-binding cassette transporter protein whose operation is for export of phospholipids and cholesterol, outside cells where they are limited to Apolipoprotein A1 (apoA1). Increased ABCA1 activity could inhibit atherosclerosis.Objectives:In the present study, the effect of aerobic exercise was investigated on gene expression and biochemical parameters.Patients and Methods:The participants included 36 inactive women, which were randomly assigned to control (CON) and experimental (EX) groups. The EX group performed 12 weeks of aerobic exercise and the CON group remained inactive. Fasting blood samples were collected 24 hours before the first session and 48 hours after completion of the course. The ABCA1 and APOA1 gene expressions were measured using semi-quantitative-RT-PCR. Data were analyzed by the SPSS software (version 18).Results:A significant increase in blood ABCA1 (EX group P < 0.002, t = - 9.876) and Apo A-I (EX group P < 0.05, t = 2.76) gene expression was shown following the 12 weeks of training. Plasma high-density lipoprotein-cholesterol (HDL-C) concentration increased (P < 0.001, t = 4.90 respectively) while plasma low-density lipoprotein-cholesterol (LDL-C) concentration decreased (P < 0.001, t = 4.27) in the EX group compared with the CON group.Conclusions:Aerobic exercises can increase ABCA1 and APO-A1 gene expression. Induction of these genes can effectively prevent cardiovascular disease.

Effects of aerobic exercise on lipids and lipoproteins

“…Study found that the ABCA1 gene expression was significantly different before and after exercise [69]. Ghanbari-Niaki et al [70] also found that ABCA1 mRNA expression increased regardless of the intensity of exercise. Therefore, they hypothesized that aerobic exercise may increase the expression of ABCA1 to exert its role in reducing cardiovascular risk.…”

Mitochondrial accumulation of doxorubicin in cardiac and diaphragm muscle following exercise preconditioning

“…Indeed, studies suggest that altering the cardiac expression of specific ABC transporters can significantly alter the rate of DOX clearance from the heart (Dell'Acqua et al, 1999;van Asperen et al, 1999). In addition, the expression of several different ABC transporters has been demonstrated to be upregulated in response to exercise training (Tofighi et al, 2015). Therefore, an exercise-induced increase in the expression of ABC transport proteins may be responsible for the protective effects of exercise on the heart.…”

Expression of ABC transporter and scavenger receptor mRNAs in PBMCs in 100‐km ultramarathon runners

“…However, exercise‐regulated cellular cholesterol efflux might depend on the different doses of aerobic exercise. Habitual aerobic exercise can increase cellular ABCA1 gene expression 20,21 . A higher ABCA1 expression was observed in low to moderate circuit‐resistance exercises than in high‐resistance exercise 22 .…”

“…Exercise has benefits in terms of reducing cardiovascular risk 19 . High levels of habitual exercise are associated with high ABCA1 expression in human leucocytes 20,21 . A single session of circuit resistance exercise increases ABCA1 expression in peripheral blood lymphocytes 22 .…”