The Effect of Quantitative Ratio Between Primer Pairs on Pcr Products in Multi-Target Amplification

Bercovich, Dani; Regev, Zipi; Ratz, Tal; Plotsky, Y.

doi:10.1007/978-1-4615-5379-3_9

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Amplification Of the Cdna Of Gth-i Gth-ii And 18s Rrna Each1

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1999

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“…Amplification of the 18S rRNA cDNA in the same tube with either GtH-I or GtH-II cDNA caused a reduction in one or both PCR products. Such interference problems are not uncommon in multiplex PCR, as reported by Bercovich et al (1997). We have carefully calibrated the concentration of each primer pair and the number of cycles for amplifications which were linearly dependent on the initial concentration of the target cDNA.…”

Section: Amplification Of the Cdna Of Gth-i Gth-ii And 18s Rrna Eachmentioning

confidence: 99%

Blue gourami (Trichogaster trichopterus) gonadotropic beta subunits (I and II) cDNA sequences and expression during oogenesis

Jackson

Goldberg²,

Ofir³

et al. 1999

Journal of Molecular Endocrinology

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We have cloned two cDNAs from the pituitary gland of blue gourami (Trichogaster trichopterus), coding for the subunits of the gonadotropin hormones GtH-I and GtH-II. The two cDNAs were sequenced and subjected to sequence analysis. We have found that the deduced amino acid sequences of both cDNAs were most similar to their striped bass counterparts. The GtH-I subunits of blue gourami and striped bass shared 73% of their residues, and the GtH-II subunits 84%. The cloning of the cDNAs ofGtH-I and GtH-II has enabled us to measure the expression of their respective mRNAs in the pituitaries of female blue gourami at different stages of the reproductive cycle. The highest levels of GtH-I and GtH-II mRNA were found in specimens classified as high vitellogenic and in females that were at the final stages of oocyte maturation.

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Section: Amplification Of the Cdna Of Gth-i Gth-ii And 18s Rrna Eachmentioning

confidence: 99%

Blue gourami (Trichogaster trichopterus) gonadotropic beta subunits (I and II) cDNA sequences and expression during oogenesis

Jackson

Goldberg²,

Ofir³

et al. 1999

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

The Effect of Quantitative Ratio Between Primer Pairs on Pcr Products in Multi-Target Amplification

Cited by 1 publication

References 9 publications

Blue gourami (Trichogaster trichopterus) gonadotropic beta subunits (I and II) cDNA sequences and expression during oogenesis

Blue gourami (Trichogaster trichopterus) gonadotropic beta subunits (I and II) cDNA sequences and expression during oogenesis

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