Since Harris et al. (1992) first observed an increase in skeletal muscle creatine (Cr) content following several days of oral supplementation, many investigators have studied the effect of short-term Cr ingestion on exercise performance. Most of the reports suggest that Cr supplementation enhances exercise performance (Balsom et al. 1993;Greenhaff et al. 1993Greenhaff et al. , 1994Birch et al. 1994;Earnest et al. 1995;Prevost et al. 1997;Maganaris & Maughan, 1998), although a number of studies have shown no effect (Febbraio et al. 1995;Redondo et al. 1996;Vandenberghe et al. 1996). In part, it seems the disparity in the findings occurs because of differences in the exercise model employed, or the intensity, duration, and frequency of the task, and the level of fitness of the subjects. Most studies have adopted cycling, swimming, or dynamic isotonic contractions, but few studies have employed an isometric model (van Leemputte et al. 1999). Compared with a dynamic task, the isometric model offers greater ability to assess in a controlled situation, the effects of various neural and contractile factors which might be affected by Cr supplementation. Motivation and effort are also important determinants of voluntary muscular output and are difficult to assess, especially during intense dynamic exercise. Thus, besides measurements of both voluntary and stimulated contractile properties, the isometric model permits the use of the twitch interpolation technique (explained in Methods) as a means of assessing indirectly subject effort, and to separate the influence of central and peripheral neuromuscular factors in the generation of muscle force during rest and exercise. Although a well-known technique in many studies of neuromuscular function (Bigland-Ritchie et al. 1986;Hales & Gandevia, 1988), no previous studies have utilized this technique with Cr supplementation. Finally, results obtained using the isometric model may help direct the design of future dynamic or performance studies. It is known that phosphocreatine (PCr) content in skeletal muscle is increased following Cr supplementation ), but the mechanism by which Cr might influence exercise performance is not known. Most research has centred upon the creatine kinase reaction (Casey et al. 1996), whereby increased availability of PCr enhances ATP resynthesis during There have been several studies on the effect of short-term creatine (Cr) supplementation on exercise performance, but none have investigated both voluntary and stimulated muscle contractions in the same experiment. Fourteen moderately active young men (19-28 years) were randomly assigned, in a double blind manner, to either a creatine (Cr) or placebo (P) group. The subjects supplemented their regular diet 4 times a day for 5 days with either 5 g Cr + 5 g maltodextrin (Cr group), or 5 g maltodextrin (P group). Isometric maximal voluntary contraction (MVC), muscle activation, as assessed using the modified twitch interpolation technique, electrically stimulated contractile properties, electromyography ...