The Effect of Lysophosphatidic Acid-supplemented Culture Medium on Human Immature Oocytes Matured in Vitro

Xie, Qingji; Xing, Yaxin; Wu, Jie; Chian, Ri‐Cheng

doi:10.21203/rs.3.rs-196908/v1

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…2A). We also tested two widely-used RNA-seq pipelines, Bowtie2 + featureCounts ( 30-35 ) and HISAT2 + featureCounts ( 36-38 ). However, these pipelines showed lower R2 = 0.963∼0.990 (Fig.…”

Section: Resultsmentioning

confidence: 99%

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A stable reference human transcriptome and proteome as a standard for reproducible omics experiments

Lü²,

Zheng³

et al. 2022

Preprint

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In recent years, the development of high-throughput omics technology has greatly promoted the development of biomedicine. However, the poor reproducibility of omics techniques limits its application. It is necessary to use standard reference materials of complex RNAs or proteins to test and calibrate the accuracy and reproducibility of omics workflows. However, the transcriptome and proteome of most cell lines shift during culturing, which limits their applicability to serve as standard samples. In this study, we demonstrated that the human hepatocellular cell line MHCC97H has a very stable transcriptome (R2=0.966-0.995) and proteome (R2=0.934-0.976 for DDA, R2=0.942-0.986 for DIA) after 9 subculturing generations, which allows this stable standard sample to be stably produced on an industrial scale for several decades. Moreover, this stability was maintained across labs and platforms. In sum, our results justified a omics standard reference material and reference datasets for transcriptomic and proteomics research. This helps to further standardize the workflow and data quality of omics techniques and thus promotes the application of omics technology in precision medicine.

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Section: Resultsmentioning

confidence: 99%

“…+ featureCounts (30)(31)(32)(33)(34)(35) and HISAT2 + featureCounts (36)(37)(38). However, these pipelines showed lower R2 = 0.963~0.990 (Fig.…”

Section: Robustness Of the Mhcc97h As A Transcriptome Referencementioning

confidence: 93%

A stable reference human transcriptome and proteome as a standard for reproducible omics experiments

Lü²,

Zheng³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition to the oocyte maturation medium itself, the medium was supplemented with 7.5 IU/mL hMG. This means that apart from the medium itself, important factors for oocyte maturation may also come from gonadotropins and other factors [48,49] . Therefore, this short pre-IVF incubation time, apart from optimum to mature oocytes, resulted in a significant increase in blastocyst development rate (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Effect of pre-IVF incubation in maturation medium on oocyte maturity, fertilization, embryonic development, and clinical outcomes following embryo transfer

Li¹,

Wang²,

Jiao³

et al. 2022

Reproductive and Developmental Medicine

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Objective: Collected human cumulus-oocyte complexes (COCs) are usually inseminated after 4 to 6 hours in in vitro fertilization (IVF) laboratories. The purpose of this study was to determine the effect of short-term pre-IVF incubation in culture medium on subsequent oocyte maturation, fertilization, and embryonic development, as well as clinical outcomes. Methods: Sixty patients were divided randomly into 2 groups, pre-IVF incubation for 5 hours: 1) with (+) the designed oocyte maturation medium; 2) without (−) the designed oocyte maturation medium (transferred directly to fertilization medium for 5 hours before insemination). Oocyte maturation and fertilization were assessed, and the rate of cleavage and good quality embryos were evaluated between the 2 groups on days 2 and 3, respectively. Blastocyst development was based on the remaining number of embryos on day 3, continuously cultured to day 5 after embryo transfer or frozen on day 3, and was compared between the 2 groups. Clinical pregnancy, implantation, and miscarriage rates were also compared. Results: Oocyte maturation rates did not differ between groups (85.8 ± 14.1% vs. 90.7 ± 9.1%). However, the range of oocyte maturation rates (58.3%–100.0%) for each patient was significantly higher in the (−) group than in the (+) pre-incubation group (71.4%–100.0%). There were no differences in fertilization rates (89.9 ± 10.0% vs. 86.5 ± 12.2%) and good quality embryos (70.8 ± 19.1% vs. 62.1 ± 23.7%) between groups; however, the blastocyst development rates were significantly different between groups (73.1 ± 20.1% vs. 58.8 ± 18.2%, P <0.05). Nevertheless, clinical pregnancy (62.5% vs. 61.1%) and implantation (46.9% vs. 47.2%) rates did not differ between groups. Conclusions: These results indicate that a short pre-IVF incubation time in the designed culture medium promotes oocyte maturation and embryonic development, suggesting that short pre-IVF incubation of COCs in the designed culture medium may be important for subsequent final oocyte maturation and early embryonic development.

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The Effect of Lysophosphatidic Acid-supplemented Culture Medium on Human Immature Oocytes Matured in Vitro

Cited by 2 publications

References 39 publications

A stable reference human transcriptome and proteome as a standard for reproducible omics experiments

A stable reference human transcriptome and proteome as a standard for reproducible omics experiments

Effect of pre-IVF incubation in maturation medium on oocyte maturity, fertilization, embryonic development, and clinical outcomes following embryo transfer

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