The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro

Abstract

Background Lysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown. Methods A total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good health, were included in this study. Immature oocytes were obtained and cultured with 10 µM lysophosphatidic acid. After culture, oocyte maturation was assessed and oocytes and cumulus cells were collected for RNA sequencing. Hierarchical indexing for spliced alignment of transcripts 2 method was used to align clean reads to the human genome. The featureCounts and edgeR package were used to calculate gene expression and analyze differences between groups respectively. ClusterProfiler program was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Results Oocyte maturation rate increased significantly following 48 h culture with lysophosphatidic acid. In cumulus cells, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in TNF signaling and insulin secretion pathways and downregulated genes were enriched in TNF signaling and cell adhesion molecules. In oocytes, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in MAPK signaling, gap junction, and cell cycle pathways and downregulated genes were enriched in MAPK signaling, estrogen signaling, RAP1 signaling, and gap junction pathways. Conclusions Lysophosphatidic acid in culture medium enhances human oocyte maturation in vitro and the identified some potential pathways may associate with oocyte maturation.

Effect of pre-IVF incubation in maturation medium on oocyte maturity, fertilization, embryonic development, and clinical outcomes following embryo transfer

“…In addition to the oocyte maturation medium itself, the medium was supplemented with 7.5 IU/mL hMG. This means that apart from the medium itself, important factors for oocyte maturation may also come from gonadotropins and other factors [48,49] . Therefore, this short pre-IVF incubation time, apart from optimum to mature oocytes, resulted in a significant increase in blastocyst development rate (Table 3).…”

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans

“…In our study, consistent results showed higher levels of ACPP in mature oocytes. Lysophosphatidic acid (LPA) has been recognized as an enhancer in human oocyte maturation in vitro (29) and can be inactivated by ACPP (30). We hypothesized that in matured MII oocytes, rising levels of ACPP in follicular fluid may act to remove an excessive maturational effect of LPA.…”

Integrated ONT Full-Length Transcriptome and Metabolism Reveal the Mechanism Affecting Ovulation in Muscovy Duck (Cairina moschata)

“…LPA is released in the endometrium of cattle, induces the expression of COX-2, progesterone, and PGE2, and is significantly increased during human pregnancy (63). Moreover, numerous studies have suggested that LPA may plays a role in oocytes (64). In human oocytes cultured in vitro, the addition of lysophosphatidic acid to immature oocytes increased cell cycle protein B1 levels in MI and MII stage oocytes and boosted the maturation rate of oocytes in vitro considerably (62).…”