The effect ofRhazya strictaaqueous leaves extract on MRSA genotypes in Jeddah province

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that is resistant to a large group of beta-lactam antibiotics. Rhazya stricta is a local shrub that grows naturally as a normal flora and is used as a medicinal plant by several nations for a lot of infectious diseases, caused by microorganisms. Therefore, the effect of the plant against different genotypes of methicillinresistant S. aureus was tested in the present study. Molecular identification was done for the medical sampling of 44 MRSA and biodiversity approaches were applied to detect the mecA gene. The 16S rRNA genes analysis was performed for the construction of a phylogenetic tree. Later on, the antimicrobial effect of the plant leaves' water extract was tested on different genotypes. MecA gene appeared in all isolates, except in methicillin-susceptible Staphylococcus aureus. The selected MRSA 16S rRNA sequences were sent to GenBank and six accession numbers (KM893010, KM893011, KP091274, KP091275, KP137513 and KP137514) were acquired. Also, an evolutionary analysis of these strains was done and a phylogenetic tree was constructed. Plant extracts showed that the interaction between pathogens and drugs is more efficient in a liquid environment than in a solid one.

Anti-Proliferative, Cytotoxic and Antioxidant Properties of the Methanolic Extracts of Five Saudi Arabian Flora with Folkloric Medicinal Use: Aizoon canariense, Citrullus colocynthis, Maerua crassifolia, Rhazya stricta and Tribulus macropterus

“…Rhazya stricta belongs to the family of Apocynaceae and is known locally as "harmal." It has traditionally been used to treat microbial infections, sore throat, diabetes mellitus, and certain inflammatory conditions such as rheumatism and allergies [7,24,25]. Previous phytochemical studies on R. stricta have shown the presence of alkaloids, flavonoids, glycosides, peptides, and triterpenes [26].…”

Larvicidal effects of some natural products on the Aedes aegypti mosquito, a vector of dengue fever and Zika virus

“…Therefore, much research is being conducted to develop effective and low‐cost control methods that are friendly to the environment and humans; among these methods is the development of insecticides from natural sources (Baeshen et al . 2019; Barnawi et al . 2019; El‐Naggar & Hasaballah 2018; Farag et al .…”

“…Globally, attention is currently focused on reducing the continuous use of chemical pesticides, which have produced environmental pollution and health effects at all levels. Therefore, much research is being conducted to develop effective and low-cost control methods that are friendly to the environment and humans; among these methods is the development of insecticides from natural sources (Baeshen et al 2019;Barnawi et al 2019;El-Naggar & Hasaballah 2018;Farag et al 2020).…”

Effects of Rhazya Stricta plant organic extracts on human induced pluripotent stem cells derived neural stem cells

“…Although numerous plant natural products have been remarkably shown to be effective treatments for neurodegenerative diseases, previously classified as noncurable diseases [15][16][17], there are still a large number of plants in ecologically diverse areas such as Saudi Arabia that have not yet been discovered for their potential therapeutic efficacy. The harsh environmental conditions in Saudi Arabia, such as heat and lack of water, force the plants to evolve and develop "survival molecules" and metabolites [18].…”