The effect of hyaluronan on mouse intramembranous osteogenesis in vitro

Pilloni, Andrea; Bernard, G. W.

doi:10.1007/s004410051182

Cited by 61 publications

(69 citation statements)

References 51 publications

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“…For example, low molecular weight HA itself appears to increase the osteogenic potential of mesenchymal cells. 66 Thus, HA or peptide-HA might find application in bone fixation devices or artificial joints where bone ingrowth could strengthen the bone-metal interface.…”

Section: Ha Attachmentmentioning

confidence: 99%

Attachment of hyaluronan to metallic surfaces

Pitt

Morris

Mason

et al. 2003

J Biomedical Materials Res

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Metal implants are in general not compatible with the tissues of the human body, and in particular, blood exhibits a severe hemostatic response. Herein we present results of a technique to mask the surface of metals with a natural biopolymer, hyaluronan (HA). HA has minimal adverse interactions with blood and other tissues, but attachment of bioactive peptides can promote specific biological interactions. In this study, stainless steel was cleaned and then surface-modified by covalent attachment of an epoxy silane. The epoxy was subsequently converted to an aldehyde functional group and reacted with hyaluronan through an adipic dihydrazide linkage, thus covalently immobilizing the HA onto the steel surface. Fluorescent labeling of the HA showed that the surface had a fairly uniform covering of HA. When human platelet rich plasma was placed on the HA-coated surface, there was no observable adhesion of platelets. HA derivatized with a peptide containing the RGD peptide sequence was also bound to the stainless steel. The RGD-containing peptide was bioactive as exemplified by the attachment and spreading of platelets on this surface. Furthermore, when the RGD peptide was replaced with the nonsense RDG sequence, minimal adhesion of platelets was observed. This type of controlled biological activity on a metal surface has potential for modulating cell growth and cellular interactions with metallic implants, such as vascular stents, orthopedic implants, heart valve cages, and more.

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Section: Ha Attachmentmentioning

confidence: 99%

Attachment of hyaluronan to metallic surfaces

Pitt

Morris

Mason

et al. 2003

J Biomedical Materials Res

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“…Our evaluation of the effect of HA concentrations on MSCs indicates that HA affected MSC proliferation in a dose-dependent manner. Data in the literature shows that the addition of small concentrations of low molecular weight HA stimulates MSC proliferation [42,43], while the same concentration of high molecular weight HA appears to inhibit the cells [44,45]. Our experiments with high molecular weight HA in a 2D system did not show any significant effect on MSC proliferation (data not shown) while cells cultured on a calcite scaffold in a 3D system in the presence of HA of various concentrations displayed a reduction in cell number.…”

Section: Effect Of Ha Concentration On Msc Proliferationmentioning

confidence: 42%

Calcite Biohybrids as Microenvironment for Stem Cells

2012

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Abstract:A new type of composite 3D biomaterial that provides extracellular cues that govern the differentiation processes of mesenchymal stem cells (MSCs) has been developed. In the present study, we evaluated the chondrogenecity of a biohybrid composed of a calcium carbonate scaffold in its calcite polymorph and hyaluronic acid (HA). The source of the calcite scaffolding is an exoskeleton of a sea barnacle Tetraclita rifotincta (T. rifotincta), Pilsbry (1916). The combination of a calcium carbonate-based bioactive scaffold with a natural polymeric hydrogel is designed to mimic the organic-mineral composite of developing bone by providing a fine-tuned microenvironment. The results indicate that the calcite-HA interface creates a suitable microenvironment for the chondrogenic differentiation of MSCs, and therefore, the biohybrid may provide a tool for tissue-engineered cartilage.

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“…To this end, RC cells were pulse-treated with either LIF or vehicle (PBS) for 3 consecutive days, within (days 7-10) and outside (days 10-13) the sensitive time window; as expected, LIF inhibited bone nodule formation only in cultures treated chronically (days [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] or in the inhibition-sensitive window, but not the insensitive window (Fig. 1).…”

Section: Identification Of Genes Modulated By Lif In a Differentiatiomentioning

confidence: 74%

“…(12) RNA purification and cDNA preparation RNA was extracted from cultured RC cells with the Trizol Reagent (Invitrogen Canada, Burlington, Ontario, Canada) according to the manufacturer's protocol and treated with DNase I (Fermentas Canada, Burlington, Ontario, Canada) for 30 min at 37°C followed by an RNA cleanup step with the RNeasy MinElute Cleanup Kit (QiagenCanada, Mississauga, Ontario, Canada). Two micrograms of DNA-free purified RNA were reversetranscribed with the SuperScript II RNase H -Reverse Transcriptase (Invitrogen Canada) and oligo(dT) [12][13][14][15][16][17][18] primers (Amersham Bioscience, Baie d'Urfé, Canada).…”

Section: Histochemistry For Detection Of Bone Nodulesmentioning

confidence: 99%

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LIF Inhibits Osteoblast Differentiation at Least in Part by Regulation of HAS2 and Its Product Hyaluronan

Falconi

Aubin

2007

Journal of Bone and Mineral Research

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LIF arrests osteogenesis in fetal rat calvaria cells in a differentiation stage-specific manner. Differential display identified HAS2 as a LIF-induced gene and its product, HA, modulated osteoblast differentiation similarly to LIF. Our data suggest that LIF arrests osteoblast differentiation by altering HA content of the extracellular matrix.Introduction: Leukemia inhibitory factor (LIF) elicits both anabolic and catabolic effects on bone. We previously showed in the fetal rat calvaria (RC) cell system that LIF inhibits osteoblast differentiation at the late osteoprogenitor/early osteoblast stage. Materials and Methods: To uncover potential molecular mediators of this inhibitory activity, we used a positive-negative genome-wide differential display screen to identify LIF-induced changes in the developing osteoblast transcriptome. Results: Although LIF signaling is active throughout the RC cell proliferation-differentiation sequence, only a relatively small number of genes, in several different functional clusters, are modulated by LIF specifically during the LIF-sensitive inhibitory time window. Based on their known and predicted functions, most of the LIF-regulated genes identified are plausible candidates to be involved in the LIF-induced arrest of osteoprogenitor differentiation. To test this hypothesis, we further analyzed the function of one of the genes identified, hyaluronan synthase 2 (HAS2), in the LIF-induced inhibition. Synthesis of hyaluronan (HA), the product of HAS enzymatic activity, was stimulated by LIF and mimicked the HAS2 expression profile, with highest expression in early/proliferative and late/maturing cultures and lowest levels in intermediate/late osteoprogenitor-early osteoblast cultures. Exogenously added high molecular weight HA, the product of HAS2, dose-dependently inhibited osteoblast differentiation, with pulse-treatment effective in the same differentiation stage-specific inhibitory window as seen with LIF. In addition, however, pulse treatment with HA in early cultures slightly increased bone nodule formation. Treatment with hyaluronidase, on the other hand, stimulated bone nodule formation in early cultures but caused a small dose-dependent inhibition of osteoblast differentiation in the LIF-and HA-sensitive late time window. Conclusions: Together the data suggest that osteoblast differentiation is acutely sensitive to HA levels and that LIF inhibits osteoblast development at least in part by stimulating high molecular weight HA synthesis through HAS2.

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The effect of hyaluronan on mouse intramembranous osteogenesis in vitro

Cited by 61 publications

References 51 publications

Attachment of hyaluronan to metallic surfaces

Attachment of hyaluronan to metallic surfaces

Calcite Biohybrids as Microenvironment for Stem Cells

LIF Inhibits Osteoblast Differentiation at Least in Part by Regulation of HAS2 and Its Product Hyaluronan

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