The Effect of Fucoidan, a Potential New, Natural, Anti-Neoplastic Agent on Uterine Sarcomas and Carcinosarcoma Cell Lines: ENITEC Collaborative Study

Abstract

The aim of the study was to assess the activity of fucoidan on the uterine sarcomas (MES-SA and ESS-1) and carcinosarcoma cell lines (SK-UT-1 and SK-UT-1B) and its toxicity on the human skin fibroblasts (HSF). Two uterine sarcomas and two carcinosarcoma cell lines were examined, as a control HSF were used. Cell viability was assessed with MTT test, apoptosis with caspase-3 activity and cell cycle by assessment of DNA synthesis. Fucoidan significantly decreases cell viability in SK-UT-1, SK-UT-1B, and ESS-1 cell lines, such effect was not observed in MES-SA. Fucoidan was not substantially affecting proliferation among normal cells. The tested agent induced apoptosis in all cell cultures used in the experiment. Fucoidan affects cell cycle of all tested cell lines except MES-SA by increasing percentage of cells in G0/sub-G1/G1 phase. Fucoidan do not only affect proliferation but induces apoptosis in selected uterine sarcoma and carcinosarcoma cell lines, so it has potential to be used as cytotoxic agent. Fucoidan seems to be promising anti-cancer agent for endometrial stromal sarcoma and carcinosarcoma.Electronic supplementary materialThe online version of this article (10.1007/s00005-019-00534-9) contains supplementary material, which is available to authorized users.

Isobolographic Analysis Demonstrates the Additive and Synergistic Effects of Gemcitabine Combined with Fucoidan in Uterine Sarcomas and Carcinosarcoma Cells

“…They concluded that fucoidan "enhances the anti-cancer activity" of tested cytostatic drugs. Furthermore, they observed the induction of apoptosis and cell cycle arrest among cells treated with fucoidan what corresponds with our previous findings [9,33].…”

“…Labeled cells were analyzed by flow cytometer FACSCalibur (Becton Dickinson, Franklin Lakes, NJ, USA), operating with CellQuest software (BD Biosciences, San Jose, CA, USA) to quantitatively assess the caspase-3 activity. The methodology of apoptosis assessment were described in detail elsewhere [9].…”

“…As we previously reported fucoidan significantly affects SK-UT-1, SK-UT-1B, and ESS-1 cell lines, meanwhile MES-SA cells seem to be resistant for this agent. IC 50 was 0.966, 3.348, and 0.848 mg/mL respectively, it was not possible to determine IC 50 for fucoidan in MES-SA cell line due to insufficient response to treatment [9]. The IC 50 values are summarized in Supplementary Table S1.…”

“…Cancers 2020, 12, x FOR PEER REVIEW 5 of 17 mg/ml respectively, it was not possible to determine IC50 for fucoidan in MES-SA cell line due to insufficient response to treatment [9]. The IC50 values are summarized in Supplementary Table S1.…”

“…Additionally, fucoidan is widely using as a dietary supplement. The characteristics listed above allow it to be considered as a safe product [8][9][10].…”