The effect of extracellular matrix properties on polarized light-based analysis of collagen fiber alignment in soft tissues

Iannucci, Leanne E.; Riak, Matthew B.; Lake, Spencer P.

doi:10.1117/12.2614438

Cited by 7 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transmission-mode quantitative polarized light imaging (QPLI) was performed on Safranin-O stained sections of IVD as previously described. 41 , 42 In brief, a SugarCube White LED Illuminator (Edmund Optics, Barrington, NJ, USA) with a Dolan-Jenner fiber optic backlight (Edmund Optics, Barrington, NJ, USA) was used to illuminate the histological section of interest. The light passed through a circular polarizing film (Edmund Optics, Barrington, NJ, USA) before being transmitted through the slide.…”

Section: Methodsmentioning

confidence: 99%

Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…High speed acquisition of fiber information over entire tissue samples is critical to understand fiber kinematics during continuous loading and evaluate structure-function relationships in soft tissues. Previous QPLI work has provided information about the fiber kinematics and organization in skin ( Woessner et al, 2019 ), ligaments ( Quinn and Winkelstein, 2008 , 2009 , 2011 ; Quinn et al, 2010 ; York et al, 2014a ; Smith et al, 2019 ), tendon ( Lake et al, 2009 ; Lake et al, 2010 ; Spiesz and Zysset, 2015 ; Zellers et al, 2021 ), heart valve tissue ( Robinson and Tranquillo, 2009 ; Tandon et al, 2020 ), tendon-to-bone interfaces ( Wu et al, 2018 ), and soft tissue analogs and gels ( Tower et al, 2002 ; Lake and Barocas, 2011 ; Sander et al, 2011 ; Iannucci et al, 2022 ). The ssQPLI system increases acquisition speed compared to previous systems without sacrificing resolution or signal-to-noise ratio, which will be particularly helpful for evaluating the time-dependent mechanical properties of viscoelastic tissues, and assessing kinematics during dynamic loading scenarios, such as tendon or ligament injuries.…”

Section: Discussionmentioning

confidence: 99%

Single shot quantitative polarized light imaging system for rapid planar biaxial testing of soft tissues

Blair

Quinn

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Quantitative Polarized Light Imaging (QPLI) is an established technique used to compute the orientation of collagen fibers based on their birefringence. QPLI systems typically require rotating linear polarizers to obtain sufficient data to estimate orientation, which limits acquisition speeds and is not ideal for its application to mechanical testing. In this paper, we present a QPLI system designed with no moving parts; a single shot technique which is ideal to characterize collagen fiber orientation and kinematics during mechanical testing. Our single shot QPLI system (ssQPLI) sorts polarized light into four linear polarization states that are collected simultaneously by four cameras. The ssQPLI system was validated using samples with known orientation and retardation, and we demonstrate its use with planar biaxial testing of mouse skin. The ssQPLI system was accurate with a mean orientation error of 1.35° ± 1.58°. Skin samples were tested with multiple loading protocols and in each case the mean orientation of the collagen network reoriented to align in the direction of primary loading as expected. In summary, the ssQPLI system is effective at quantifying collagen fiber organization, and, when combined with mechanical testing, can rapidly provide pixel-wise measures of fiber orientation during biaxial loading.

show abstract

“…Transmission mode QPLI was performed as previously described 27,28 . Briefly, unstained histological sections of tendon were illuminated with circularly polarized white light (Edmund Optics).…”

Section: Methodsmentioning

confidence: 99%

“…Transmission mode QPLI was performed as previously described. 27,28 Briefly, unstained histological sections of tendon were illuminated with circularly polarized white light (Edmund Optics). The illuminated section was imaged using a division of focal plane polarization camera (FLIR).…”

Section: Quantitative Polarized Light Imaging (Qpli)mentioning

confidence: 99%

The effects of NF‐κB suppression on the early healing response following intrasynovial tendon repair in a canine model

Lane

Migotsky

Havlioglu

et al. 2023

Journal Orthopaedic Research

View full text Add to dashboard Cite

The highly variable clinical outcomes noted after intrasynovial tendon repair have been associated with an early inflammatory response leading to the development of fibrovascular adhesions. Prior efforts to broadly suppress this inflammatory response have been largely unsuccessful. Recent studies have shown that selective inhibition of IkappaB kinase beta (IKK‐β), an upstream activator of nuclear factor kappa‐light chain enhancer of activated B cells (NF‐κB) signaling, mitigates the early inflammatory response and leads to improved tendon healing outcomes. In the current study, we test the hypothesis that oral treatment with the IKK‐β inhibitor ACHP (2‐amino‐6‐[2‐(cyclopropylmethoxy)‐6‐hydroxyphenyl]‐4‐piperidin‐4‐yl nicotinenitrile an inhibitor) will modulate the postoperative inflammatory response and improve intrasynovial flexor tendon healing. To test this hypothesis, the flexor digitorum profundus tendon of 21 canines was transected and repaired within the intrasynovial region and assessed after 3 and 14 days. Histomorphometry, gene expression analyses, immunohistochemistry, and quantitative polarized light imaging were used to examine ACHP‐mediated changes. ACHP led to reduction in phosphorylated p‐65, indicating that NF‐κB activity was suppressed. ACHP enhanced expression of inflammation‐related genes at 3 days and suppressed expression of these genes at 14 days. Histomorphometry revealed enhanced cellular proliferation and neovascularization in ACHP‐treated tendons compared with time‐matched controls. These findings demonstrate that ACHP effectively suppressed NF‐κB signaling and modulated early inflammation, leading to increased cellular proliferation and neovascularization without stimulating the formation of fibrovascular adhesions. Together, these data suggest that ACHP treatment accelerated the inflammatory and proliferative phases of tendon healing following intrasynovial flexor tendon repair. Clinical Significance: Using a clinically relevant large‐animal model, this study revealed that targeted inhibition of nuclear factor kappa‐light chain enhancer of activated B cells signaling with ACHP provides a new therapeutic strategy for enhancing the repair of sutured intrasynovial tendons.

show abstract

The effect of extracellular matrix properties on polarized light-based analysis of collagen fiber alignment in soft tissues

Cited by 7 publications

References 10 publications

Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc

Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc

Single shot quantitative polarized light imaging system for rapid planar biaxial testing of soft tissues

The effects of NF‐κB suppression on the early healing response following intrasynovial tendon repair in a canine model

Contact Info

Product

Resources

About