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Cited by 97 publications
(26 citation statements)
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“…The recombinant enzymes were purified by affinity chromatogra-compared with the wild-type A␤ 1-40 (data not shown). Previously we have shown that A␤ peptide binds AChE phy from the medium of transfected HEK-293 cells (Velan et al, 1991;Bourne et al, 1995). Mouse AChE (Inestrosa et al, 1994, LatinoAmerican Congress of Pharmacology, abstract;Alvarez et al, 1995).…”
Section: Characterization Of the Amyloid Fibrils Induced By Achementioning
confidence: 85%
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“…The recombinant enzymes were purified by affinity chromatogra-compared with the wild-type A␤ 1-40 (data not shown). Previously we have shown that A␤ peptide binds AChE phy from the medium of transfected HEK-293 cells (Velan et al, 1991;Bourne et al, 1995). Mouse AChE (Inestrosa et al, 1994, LatinoAmerican Congress of Pharmacology, abstract;Alvarez et al, 1995).…”
Section: Characterization Of the Amyloid Fibrils Induced By Achementioning
confidence: 85%
“…of catalytic subunits (Velan et al, 1991), and a G1 (4.8 S) mouse to aggregated A␤ in the form of stable amyloid plaques.…”
Section: Recombinant Achesmentioning
confidence: 99%
“…After the establishment of the anchor-dependent cultures, the cells were fed with production medium (containing 10 % FBS depleted of AChE). Conditioned medium harvested from confluent stationary cell cultures consistently contained higher proportions of tetrameric AChE (tetramer between 10 % and 15 % ; dimer between 70 % and 80 % ; monomer between 5 % and 20 % ; for example preparation 4 in Table 2) than that of young subconfluent cultures of producer cells (for example preparation 8 in Table 2), which are characterized by very high levels of dimers (more than 95 %) ( [16,17], and A. Lazar, C. Kronman, B. Velan and A. Shafferman, unpublished work).…”
Section: Enzyme Production Purification and Quantificationmentioning
confidence: 99%
“…An examination of fetal BoAChE by sucrose-gradient analysis reveals that this circulatory long-lived enzyme is arranged entirely in the tetrameric configuration. In contrast, recombinant ChEs produced in various cell culture systems contain variable amounts of tetramers, dimers and monomers [4,17,32,34,35]. For example, rHuAChE produced in HEK-293 or 293ST-2D6 cells exhibits at most 5-15 % tetramers and more than 60 % dimers of AChE subunits, suggesting that the inability of recombinant human ChE to assemble efficiently into stable tetramers might contribute to its relatively rapid removal from the circulation.…”
Section: Circulatory Residence Of Rhuache Is Affected By Its State Of Assemblymentioning
confidence: 99%
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