The effect of different platelet‐rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells <i>in vitro</i>

Han, Joungho; Meng, Hua; Tang, Jianbing; Li, S. L.; Tang, Yi; Chen, Z. B.

doi:10.1111/j.1365-2184.2007.00430.x

Cited by 100 publications

(96 citation statements)

References 32 publications

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“…PRP induces stimulation of cell growth in ASCs, periodontal ligament and mesenchymal stem cells as well as enhancement of cellular adhesion, proliferation and differentiation of human periodontal ligament cells (40). In the present study, plasma with chitosan-induced growth factor enrichment stimulated the growth of fibroblasts and ASCs.…”

Section: Discussionsupporting

confidence: 59%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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Abstract. Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that exhibits high platelet activation ability was investigated. A total of 13 chitosan samples with different molecular weight (Mw) and degree of deacetylation (DDA) were individually added to blood samples of rats and the amount of growth factors, albumin and fibrinogen in plasma was measured. To examine the influence of plasma activated by chitosan on the proliferation of fibroblasts and adipose tissue-derived stromal cells (ASCs), the plasma was added to the culture medium of human fibroblasts and adipose tissue-derived stromal cells. Chitosan with a DDA of >75% increased the release of platelet factor 4 into the plasma. The amount of growth factors released into the plasma and platelet activation varied depending on the Mw and DDA, while albumin and fibrinogen were hardly affected. The proliferation rate was highest when using plasma activated by chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da. These results suggested that the effectiveness of PRP therapy may be improved by using chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da.

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Section: Discussionsupporting

confidence: 59%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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“…We used the ACD-A tubes to make homemade PRP s after evaluating the various protocols published in the literature [1][2][3][4][5][6][7][8][9][10][11][12]. There are many protocols for PRP and different concentrations are reported while using different spin times and different g forces.…”

Section: Discussionmentioning

confidence: 99%

“…However, having similar platelet counting throughout the PRP is important for treatment. Because the main thing that provides treatment is related to the growth factors that are released from the platelets [2,3] this platelet counting is necessary after platelets in the PRP are homogeneously dispersed in the plasma. PRP is obtained in different amounts depending on the tubes used [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…PRP is obtained in different amounts depending on the tubes used [3][4][5][6]. When preparing PRP, single spin or double spin can be used [2], single spin is generally used in most clinics. Its convenience is that the sterility is more reliable as there is no tube transfer.…”

Section: Introductionmentioning

confidence: 99%

“…Of course, the problem here is how much of the platelets can be collected in the desired area while preparing the PRP, in other words how much of the platelets can be separated from the blood. Double spin is very effective for this reason [2,7,11]. When separating the blood and the plasma, we will lose least amount of platelet when we take the whole plasma.…”

Section: Introductionmentioning

confidence: 99%

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Importance of homogenization in platelet-rich plasma researches

Gökkaya

Görğü

Karanfil

et al. 2018

j-ebr

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A B S T R A C TAim: To achieve homogenization, one of the most important factors to overcome during platelet-rich plasma (PRP) is standardization phases. Method: We used 10 cc 'BD vacutainer ACD-A' tubes which consisted of 1.5 cc ACD-A. 3 tubes blood was collected from healthy volunteers from 20 to 40 years. All tubes were centrifuged by 180g x 12 min. Then 0.01 ml PRP was collected from just the level of buffy coat. Results: Platelet concentration decreased from buffy coat to top of PPP as expected. If any counting done before homogenization the results will be misleading. Conclusion: When we sample 3 cc PRP homogenized with shaker at 3 point from 3 different point, we see that they are close to each other. It is important that the PRP to be used in the studies must be homogenized before any measurement.

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Tri‐Layered Nanocomposite Hydrogel Scaffold for the Concurrent Regeneration of Cementum, Periodontal Ligament, and Alveolar Bone

Sowmya

Mony

Jayachandran

et al. 2017

Adv Healthcare Materials

123

120

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A tri-layered scaffolding approach is adopted for the complete and concurrent regeneration of hard tissues-cementum and alveolar bone-and soft tissue-the periodontal ligament (PDL)-at a periodontal defect site. The porous tri-layered nanocomposite hydrogel scaffold is composed of chitin-poly(lactic-co-glycolic acid) (PLGA)/nanobioactive glass ceramic (nBGC)/cementum protein 1 as the cementum layer, chitin-PLGA/fibroblast growth factor 2 as the PDL layer, and chitin-PLGA/nBGC/platelet-rich plasma derived growth factors as the alveolar bone layer. The tri-layered nanocomposite hydrogel scaffold is cytocompatible and favored cementogenic, fibrogenic, and osteogenic differentiation of human dental follicle stem cells. In vivo, tri-layered nanocomposite hydrogel scaffold with/without growth factors is implanted into rabbit maxillary periodontal defects and compared with the controls at 1 and 3 months postoperatively. The tri-layered nanocomposite hydrogel scaffold with growth factors demonstrates complete defect closure and healing with new cancellous-like tissue formation on microcomputed tomography analysis. Histological and immunohistochemical analyses further confirm the formation of new cementum, fibrous PDL, and alveolar bone with well-defined bony trabeculae in comparison to the other three groups. In conclusion, the tri-layered nanocomposite hydrogel scaffold with growth factors can serve as an alternative regenerative approach to achieve simultaneous and complete periodontal regeneration.

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The effect of different platelet‐rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro

Cited by 100 publications

References 32 publications

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Importance of homogenization in platelet-rich plasma researches

Tri‐Layered Nanocomposite Hydrogel Scaffold for the Concurrent Regeneration of Cementum, Periodontal Ligament, and Alveolar Bone

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