The effect of cryopreservation on the survivability, viability and motility of epididymal african buffalo (syncerus caffer) spermatozoa

Lambrechts, H.; Niekerk, F.E. Van; Coetzer, W.A.; Cloete, S.W.P.; Horst, Gerhard van der

doi:10.1016/s0093-691x(99)00201-0

Cited by 21 publications

(17 citation statements)

References 9 publications

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“…For the species in this study, with the possible exception of the African buffalo, only a small number of reports exist concerning sperm physiology, cryopreservation, or both [27][28][29][30][31][32]. The results of this study indicate that high-quality (motile and viable with an intact acrosome) epididymal spermatozoa can be recovered from blesbok, African buffalo, springbok, and black wildebeest.…”

Section: Discussionmentioning

confidence: 89%

Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids1

Herrick

Bartels

Krisher

2004

Biology of Reproduction

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An improved understanding of reproductive physiology in nondomestic bovids is necessary for the development of assisted reproductive technologies (ARTs) for use in the conservation of endangered bovids. In this study, epididymal spermatozoa were recovered from blesbok (Damaliscus dorcas phillipsi), African buffalo (Syncerus caffer), springbok (Antidorcas marsupialis), and black wildebeest (Connochaetes gnou) following organized culls in South Africa. Our objectives were 1) to characterize the quality of epididymal spermatozoa, 2) evaluate the effectiveness of a cryopreservation protocol, and 3) compare postthaw sperm longevity (motility, viability, and acrosomal integrity) and functionality in two culture media with two capacitation reagents (caffeine and heparin). Following recovery, spermatozoa were diluted in EQ extender, slow-cooled, and frozen in the presence of 5% glycerol. Thawed spermatozoa were separated on a Percoll gradient and diluted in fertilization media (SOF for fertilization [SOFfert]; 0.6% BSA, 0.0 mM glucose, 25.0 mM NaHCO(3)) or modified SOFfert (1.2% BSA, 1.5 mM glucose, 37.0 mM NaHCO(3)) and either heparin or caffeine, and incubated for 6 h. Spermatozoa from these species maintained an average of 64% initial motility after thawing. Incubation medium and capacitation reagent had species-specific effects on the motility, viability, and acrosomal integrity of spermatozoa, suggesting ART procedures need to be optimized for each species. Springbok spermatozoa were also shown to be competent for in vitro fertilization. Information from this study concerning sperm physiology in blesbok, African buffalo, springbok, and black wildebeest will be useful in the development of ART for the conservation of these and other species of bovids.

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Section: Discussionmentioning

confidence: 89%

Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids1

Herrick

Bartels

Krisher

2004

Biology of Reproduction

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“…Finally, the differences between previous results and ours could also be due to differences in the source of the spermatozoa. For ungulates, there seems to be only a previous study (Lambrechts et al, 1999), in which these species have been used as a model for the analysis of the possible effects of thawing procedure on characteristics of epididymal spermatozoa. The referred work demonstrated that the effect of cryopreservation on sperm characteristics of epididymal African buffalo spermatozoa was independent of the thawing procedure (35°C for 30 seconds vs 80°C for 5 seconds).…”

Section: Discussionmentioning

confidence: 99%

“…The resistance of spermatozoa to the thawing process is dependent on the basic extender used and the concentration of cryoprotectant as they interact with the freezing and thawing rates (Curry and Watson, 1994; Curry 2000). This sensitivity of the spermatozoa to thawing temperatures appears to differ between species (Fiser et al, 1981; Arav et al, 1994; Söderquist et al, 1997; Younis et al, 1998; Eriksson et al, 2000; Sukhato et al, 2001; Soler et al, 2003b) and between reproductive tract sources as has been shown in African buffalo sperm samples (Lambrechts et al, 1999). The effect of reproductive tract source (ejaculated vs epididymal) on sperm resistance to the thawing process might be related to different environment conditions between epididymal and ejaculated spermatozoa.…”

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confidence: 97%

“…Most procedures used to cryopreserve epididymal spermatozoa from deer have been modified from those developed for ejaculated semen. This approach would not seem to be very appropriate because it is well known that there are important differences in the physiological characteristics of epididymal vs ejaculated spermatozoa, specifically in their membrane properties, which affect the cell survival after cooling and freezing (Hammond, 1930; Walton, 1930; Watson and Morris, 1987; Rath and Niemann, 1997; Gilmore et al, 1998a; Lambrechts et al, 1999). Furthermore, epididymal spermatozoa have not been exposed to the complex secretions of the accessory sex glands; these secretions alter both the chilling sensitivity and the freezing resistance of ejaculated spermatozoa (Viruela and Rajaniemi, 1983; Schmehl et al, 1986).…”

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confidence: 99%

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Effects of Thawing Procedure on Postthawed In Vitro Viability and In Vivo Fertility of Red Deer Epididymal Spermatozoa Cryopreserved at −196°C

Soler¹,

García

Fernández-Santos³

et al. 2003

Journal of Andrology

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ABSTRACT:In this study, we have determined the effects of individual factor and thawing procedure on in vitro viability and in vivo fertility of frozen-thawed red deer epididymal spermatozoa. The spermatozoa that were collected from 13 Iberian deer stags were diluted at room temperature in a Triladylா-20% egg yolk medium and frozen in nitrogen vapors. In the first experimental series, sperm samples were collected from 10 mature stags. For thawing, the frozen straws were subjected to 3 different procedures: I (37ЊC for 20 seconds), II (60ЊC for 8 seconds) and III (70ЊC for 5 seconds). Sperm cryosurvival was judged in vitro by microscopic assessments of individual sperm motility (SM) and of plasma membrane and acrosome (NAR) integrities. Statistically significant variations were found (P Ͻ .05) between stags for most of the seminal parameters evaluated. The thawing procedure did not have an effect on the seminal characteristics evaluated after this process, except for SM (P Ͻ .05), with the best overall recovery rates after freezing and thawing found with the use of protocol I. Our results also show a differential resistance to return to isosmotic conditions of spermatozoa thawed using the different thawing protocols. In the second experimental series (insemination artificial trial), with spermatozoa from 3 stags, results of fertility were statistically higher (69.7% vs 42.4%, P ϭ .014) when spermatozoa were thawed at 37ЊC for 20 seconds than were warmed at 60ЊC for 8 seconds. Therefore, thawing protocol I, which provides slow thawing rates, was the most beneficial for epididymal spermatozoa thawing of the cervid subspecies analyzed in this study. In summary, high in vitro survival and in vivo fertility of frozenthawed deer epididymal spermatozoa were dependent on warming rates, but each stag exhibited its own sensitivity to cryopreservation.

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“…2003; Ehling et al. 2006), cattle (Foote 1978), buffalo (Lambrechts et al. 1999), goat (Blash et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

Lone¹,

Islam²,

Sofi³

2011

Reprod Domestic Animals

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Cauda epididymal spermatozoa were obtained from testicles collected from abattoir(s). The pooled sperm samples were divided into four aliquots. Each aliquot was washed separately with the buffer of respective extender and finally extended with the four extenders viz. egg yolk-citrate (EYC), egg yolk-citrate-fructose (EYCF), Tris-citric acid-egg yolk-fructose (TCEYF) and egg yolk-Mcillvaine glucose (EYMG) and preserved at 4°C. The per cent sperm motility for EYC, EYCF, TCEYF and EYMG at 0 h was 50.83%, 56.67%, 75.00% and 31.67%, respectively, and at 72 h was 24.17% (EYC), 30.83% (EYCF), 51.67% (TCEYF) and 7.50% (EYMG). The corresponding figures for live sperm count at 0 h was 83.17%, 86.33%, 90.42% and 81.75% and at 72 h was 64.75%, 73.92%, 76.00% and 57.67%. The corresponding figures for mean per cent intact acrosome at 0 h was 95.33%, 95.50%, 90.92% and 97.25% and at 72 h was 86.17%, 83.92%, 77.58% and 86.33%. The sperm motility was significantly (p < 0.05) higher for TCEYF at different h of preservation from 0 h through 72 h. The sperm motility, live sperm count and per cent intact acrosome declined significantly (p < 0.05) with the advancement of storage time in all the four extenders. Our study concluded that TCEYF was best out of the extenders studied for preservation of cauda epididymal spermatozoa after double centrifugation and extension at 4°C up to 72 h of preservation. However, EYCF also has better potential for the preservation of cauda epididymal spermatozoa as viability was in close proximity and acrosomal integrity was higher compared with TCEYF extender.

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The effect of cryopreservation on the survivability, viability and motility of epididymal african buffalo (syncerus caffer) spermatozoa

Cited by 21 publications

References 9 publications

Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids1

Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids1

Effects of Thawing Procedure on Postthawed In Vitro Viability and In Vivo Fertility of Red Deer Epididymal Spermatozoa Cryopreserved at −196°C

Effect of Different Egg Yolk‐Based Extenders on the Quality of Ovine Cauda Epididymal Spermatozoa during Storage at 4°C

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