The effect of compound 48/80 and of electrical field stimulation on mast cells in the isolated mouse stomach

Stanovnik, Lovro; Logonder-Mlinšek, M.; Erjavec, F.

doi:10.1007/bf02142570

Cited by 19 publications

(5 citation statements)

References 8 publications

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“…Iurina et al (79) suggested that the high magnetic sensitivity of MCs revealed in their study could be used as a test of body reactivity and adaptation to this physical agent! Finally, there are also reports indicating tissue-specific effects (80) as well as the appearance of EMF-modulating effects on the number of mast cells in healing skin (81). However, also single studies finding a lack of such biophysical correlations are found in the literature (see e.g.…”

Section: Direct Effects Of Emfs On Mast Cellsmentioning

confidence: 97%

A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans

Gangi

Johansson

2000

Medical Hypotheses

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Section: Direct Effects Of Emfs On Mast Cellsmentioning

confidence: 97%

A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans

Gangi

Johansson

2000

Medical Hypotheses

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“…Similarly, CTMCs were also identified in the mouse gastric submucosa. 31 Of note, C48/80, which is shown as an effective adjuvant following IN immunizations, 8,10 selectively activates CTMCs but not MMCs 26,45,46 and, hence, it has to penetrate ''deep'' into the submucosa to reach the CTMCs. Furthermore, differential responsiveness of CTMCs and MMCs to other stimuli has also been described.…”

Section: Toluidine Bluementioning

confidence: 99%

The immune complex CTA1-DD/IgG adjuvant specifically targets connective tissue mast cells through FcγRIIIA and augments anti-HPV immunity after nasal immunization

et al. 2013

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We have previously reported that CTA1-DD/IgG immune complexes augment antibody responses in a mast cell-dependent manner following intranasal (IN) immunizations. However, from a safety perspective, mast cell activation could preclude clinical use. Therefore, we have extended these studies and demonstrate that CTA1-DD/IgG immune complexes administered IN did not trigger an anaphylactic reaction. Importantly, CTA1-DD/IgE immune complexes did not activate mast cells. Interestingly, only connective tissue, but not mucosal, mast cells could be activated by CTA1-DD/IgG immune complexes. This effect was mediated by FcγRIIIA, only expressed on connective tissue mast cells, and found in the nasal submucosa. FcγRIIIA-deficient mice had compromised responses to immunization adjuvanted by CTA1-DD/IgG. Proof-of-concept studies revealed that IN immunized mice with human papillomavirus (HPV) type 16 L1 virus-like particles (VLP) and CTA1-DD/IgG immune complexes demonstrated strong and sustained specific antibody titers in serum and vaginal secretions. From a mast cell perspective, CTA1-DD/IgG immune complexes appear to be safe and effective mucosal adjuvants.

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“…MC were counted and the percentage of degranulated MC was determined in 200 cells for each preparation. MC were identified and their degranulation assessed by morphological criteria [7].…”

Section: Methodsmentioning

confidence: 99%

Is endogenous histamine involved in the contraction of the isolated trachea, evoked by muscarinic agonist?

Korošec

Stanovnik

1999

Inflammation Research

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IntroductionThere are numerous data showing the importance of mast cells (MC) in bronchial hyperreactivity [1,2]. Much evidence also supports the involvement of cholinergic mechanisms in bronchial contractility [3]. The two mechanisms could be interconnected, as there are data available showing the influence of the cholinergic system on MC secretion [4][5][6]. Therefore MC may be involved in bronchial contractions evoked by muscarinic agonists. The aim of our work was to establish whether the depletion of MC histamine by the histamine liberator compound 48/80 (C48/80) could influence contractions of guinea pig isolated trachea evoked by the muscarinic agonist carbachol. Materials and methodsPieces of 5 mm long tubes of guinea pig isolated trachea were transversely mounted in an organ bath with Tyrode solution (37°C). After 45 min equilibration, contractions, induced by carbachol or histamine, were recorded isometrically. On each preparation, a set of cumulative concentrations of either carbachol or histamine was added twicebefore and after C48/80 (100 mg/ml), which was washed out 15 min after the addition.The parameters of concentration-response curve (ED 50 , E max and the slope, p) were calculated for each set of concentrations, [A], according to the logistic function:The parameters obtained before and after C48/80 were statistically compared by t-test for repeated observations. In 3 separate groups of experiments, pieces of tracheae were examined histologically for MC degranulation. They were first placed in Tyrode solution for 30 min and then removed for preparation of histological samples (Group 1). In groups 2 and 3, C48/80 (50 mg/ml in Group 2 and 100 mg/ml in Group 3) was added in the bath for 15 min before tracheal tubes were removed for histological examination. The samples were fixed in Carnoy¢s solution and in basic lead acetate solution (1%), sectioned and stained with toluidine blue (0.5%). MC were counted and the percentage of degranulated MC was determined in 200 cells for each preparation. MC were identified and their degranulation assessed by morphological criteria [7]. Results and discussionAt histological examination, MC were found in the submucosa and also in deeper layers of the paries membranaceus tracheae, where they lay close to the smooth muscular cells. C48/80 evoked statistically significant degranulation of MC, although the difference between the two concentrations was not statistically significant (Table 1). Manipulation and exposure to Tyrode solution probably caused the rather high percentage of degranulated MC in all preparations, including the controls. The highest concentration of C48/80 provoked nearly 80% degranulation of MC and this concentration was used in the experiments where contractions were measured.C48/80 (100 mg/ml) evoked contractions that were comparable to the maximal effects of carbachol. The effect of carbachol was significantly diminished after C48/80: The maximal force of contraction (E max ) was shifted from 2.8 to 2.2 g and ED 50 from 0.086 to 0.13 mM. Howev...

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The effect of compound 48/80 and of electrical field stimulation on mast cells in the isolated mouse stomach

Cited by 19 publications

References 8 publications

A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans

A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans

The immune complex CTA1-DD/IgG adjuvant specifically targets connective tissue mast cells through FcγRIIIA and augments anti-HPV immunity after nasal immunization

Is endogenous histamine involved in the contraction of the isolated trachea, evoked by muscarinic agonist?

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