The effect of axotomy on firing and ultrastructure of the crayfish mechanoreceptor neurons and satellite glial cells

Rudkovskii, M. V.; Fedorenko, A.G.; Khaitin, Andrey; Pitinova, Maria; Uzdensky, Anatoly B.

doi:10.1016/j.mcn.2020.103534

Cited by 5 publications

(9 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This spiking, as well in in Kuwada experiment, is responsible for voltage-gated Na + conductivity, but not Ca 2+ conductivity. In our experiments on crayfish stretch receptor neurons, the firing frequency and the amplitude of action potentials did not change during first minute after the axon transection [19].…”

Section: Calcium In Electrophysiological Response To Axotomycontrasting

confidence: 45%

See 1 more Smart Citation

Calcium in Neuronal and Glial Response to Axotomy

Khaitin

2021

IJMS

View full text Add to dashboard Cite

Neurotrauma assumes an instant or delayed disconnection of axons (axotomy), which affects not only neurons, but surrounding glia as well. Not only mechanically injured glia near the site of disconnection, especially transection, is subjected to the damage, but also glia that is remote from the lesion site. Glial cells, which surround the neuronal body, in turn, support neuron survival, so there is a mutual protection between neuron and glia. Calcium signaling is a central mediator of all post-axotomy events, both in neuron and glia, playing a critical role in their survival/regeneration or death/degeneration. The involvement of calcium in post-axotomy survival of the remote, mechanically intact glia is poorly studied. The purpose of this review is to sum up the calcium-involving mechanisms in responses of neurons and glial cells to axotomy to show their importance and to give some suggestions for future research of remote glia in this context.

show abstract

Section: Calcium In Electrophysiological Response To Axotomycontrasting

confidence: 45%

“…The resulting Ca 2+ waves propagate along the axon and serve as the initial damage signal for soma. In our experiments on crayfish stretch receptor neurons, axotomy induced the elevation in cytosolic calcium levels in soma and near-soma axoplasm within minutes [19].…”

Section: Calcium Dynamics In Neurons and Glia After Axotomymentioning

confidence: 63%

Calcium in Neuronal and Glial Response to Axotomy

Khaitin

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…It is a convenient model object for studying the responses of neurons and glial cells to various damaging effects, including axotomy [ 7 ]. The ultrastructure of this neuroglial object has been studied in detail in many studies [ 26 , 27 ]. Heat shock protein 70 (Hsp70) was found in all organs of invertebrates [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

Demyanenko

Pitinova

Dzreyan

et al. 2021

Cells

View full text Add to dashboard Cite

The search for effective neuroprotective agents for the treatment of neurotrauma has always been of great interest to researchers around the world. Extracellular heat shock protein 70 (eHsp70) is considered a promising agent to study, as it has been demonstrated to exert a significant neuroprotective activity against various neurodegenerative diseases. We showed that eHsp70 can penetrate neurons and glial cells when added to the incubation medium, and can accumulate in the nuclei of neurons and satellite glial cells after axotomy. eHsp70 reduces apoptosis and necrosis of the glial cells, but not the neurons. At the same time, co-localization of eHsp70 with p53 protein, one of the key regulators of apoptosis, was noted. eHsp70 reduces the level of the p53 protein apoptosis promoter both in glial cells and in the nuclei and cytoplasm of neurons, which indicates its neuroprotective effect. The ability of eHsp70 to reverse the proapoptotic effect of the p53 activator WR1065 may indicate its ability to regulate p53 activity or its proteosome-dependent degradation.

show abstract

“…The spikes propagate to the ventral nerve cord ganglion [16][17][18] . The ultrastructure of this neuron was studied in detail in many works [19][20][21] . It proved itself a convenient model object for studying the response of neurons and glial cells to various damaging impacts including axotomy 17 .…”

Section: Methodsmentioning

confidence: 99%

Expression and Localization of C-APP and N-APP in Peripheral Neurons of Rats and Crayfish After Axotomy

Rodkin¹,

Dzreyan²,

Khaitin³

et al. 2021

Preprint

View full text Add to dashboard Cite

Nerve injury induces a cascade of molecular-cellular events, leading to neuronal death or survival, where amyloid precursor protein (APP) and its proteolytic products play an important role. We studied the localization and expression of C-APP and N-APP in rat dorsal root ganglia (DRG) with transected sciatic nerve, axotomized crayfish stretch receptor neuron (SRN) and ventral nerve cord (VNC) ganglia with transected connectives. C-APP and N-APP localized predominantly in neurons, not in glial cells. Axotomy increased C-APP and N-APP expression in rat and crayfish neurons. The expression of APP in crustaceans confirms its conservative nature. In DRG, C-APP level was higher in neuronal nuclei than in cytoplasm in 24 hours post-axotomy. N-APP accumulation was not observed in DRG and crayfish neuronal nuclei. SRN axotomy resulted in C-APP and N-APP accumulation in 4–8 hours in perikaryon and its extensions, but only С-APP accumulated in nuclei. This indicates that not the whole APP, but its C-terminal product, AICD, enters the nucleus. Also, there was high level of C-APP in SRN nucleolus, suggesting possible AICD involvement in rRNA synthesis and ribosome formation. The APP accumulation in transected axons confirms its involvement in injury-induced axonal events.

show abstract

The effect of axotomy on firing and ultrastructure of the crayfish mechanoreceptor neurons and satellite glial cells

Cited by 5 publications

References 45 publications

Calcium in Neuronal and Glial Response to Axotomy

Calcium in Neuronal and Glial Response to Axotomy

The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

Expression and Localization of C-APP and N-APP in Peripheral Neurons of Rats and Crayfish After Axotomy

Contact Info

Product

Resources

About