The effect of autophagy inhibitors on drug delivery using biodegradable polymer nanoparticles in cancer treatment

Zhang, Xudong; Dong, Yue; Zeng, Xiaowei; Liang, Xin; Li, Xiaoming; Tao, Wei; Chen, Hongbo; Jiang, Yuyang; Mei, Lin; Feng, Si‐Shen

doi:10.1016/j.biomaterials.2013.10.034

Cited by 159 publications

(125 citation statements)

References 46 publications

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“…25,26 Pharmacokinetic studies have displayed that NPs can significantly increase the intracellular concentration of anticancer drugs and still maintain a certain concentration of drug after drug withdrawal to achieve better therapeutic effect. 10,27 Many traditional Chinese medicines such as Tet and gambogic acid (GA) have antitumor effects, 8,22,28 and could reverse drug resistance, kill tumor cells, and improve immune function. In our study, tumor growth was also significantly slowed in the groups treated with NPs.…”

Section: Discussionmentioning

confidence: 99%

Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Guo

Zhang

Wang

et al. 2015

IJN

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The study investigated the reversal of multidrug resistance (MDR) and the biodistribution of nanoparticles (NPs) that target leukemia cells in a nude mice model via a surface-bound transferrin (Tf). The cytotoxic cargo of daunorubicin (DNR) and tetrandrine (Tet) was protected in the NPs by an outer coat composed of polyethylene glycol (PEG)-poly- l -lysine (PLL)-poly(lactic- co -glycolic acid) (PLGA) NPs. Injection of DNR-Tet-Tf-PEG-PLL-PLGA NPs into nude mice bearing MDR leukemia cell K562/A02 xenografts was shown to inhibit tumor growth, and contemporaneous immunohistochemical analysis of tumor tissue showed the targeted NPs induced apoptosis in tumor cells. Targeted tumor cells exhibited a marked increase in Tf receptor expression, with noticeable decreases in P-glycoprotein, MDR protein, and nuclear factor κB, as assessed by quantitative real-time polymerase chain reaction and Western blot analysis. Moreover, the concentration of DNR was shown to increase in plasma, tumor tissue, and major organs. Flow cytometry analysis with a near-infrared fluorescent (NIRF) dye, NIR797, was used to study the effectiveness of Tf as a targeting group for leukemia cells, a finding that was supported by NIRF imaging in tumor-bearing nude mice. In summary, our studies show that DNR-Tet-Tf-PEG-PLL-PLGA NPs provide a specific and effective means to target cytotoxic drugs to MDR tumor cells.

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Section: Discussionmentioning

confidence: 99%

Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Guo

Zhang

Wang

et al. 2015

IJN

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“…Untreated cells were taken as control with 100% viability, and cells without addition of MTT were used as blank to calibrate the spectrophotometer to zero absorbance. 40 In vivo antitumor efficacy and side effect analysis …”

Section: Cellular Uptake Of Npsmentioning

confidence: 99%

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Xiong¹,

Peng²,

Chen³

et al. 2015

IJN

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To enhance the therapeutic effects of chemotherapy on malignant melanoma, paclitaxel (PTX)-loaded methoxy poly(ethylene glycol)- b -poly(ε-caprolactone) nanoparticles (MPEG- b -PCL NPs) that had their surfaces modified with polydopamine (PTX-loaded MPEG- b -PCL NPs@PDA) were prepared as drug vehicles. The block copolymer MPEG- b -PCL was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded NPs were prepared by a modified nanoprecipitation technique. The PTX-loaded NPs and PTX-loaded NPs@PDA were characterized in terms of size and size distribution, zeta potential, surface morphology, drug encapsulation efficiency, and drug release. Confocal laser scanning microscopy showed that coumarin-6-loaded NPs@PDA could be internalized by human melanoma cell line A875 cells. The cellular uptake efficiency of NPs was greatly enhanced after PDA modification. The antitumor efficacy of the PTX-loaded NPs@PDA was investigated in vitro by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and in vivo by a xenograft tumor model. The PTX-loaded NPs@PDA could significantly inhibit tumor growth compared to Taxol ® and precursor PTX-loaded NPs. All the results suggested that the PTX-loaded MPEG- b -PCL NPs that had their surfaces modified with PDA are promising nanocarriers for malignant melanoma therapy.

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“…Furthermore, similarly to PLGA, it has the approval of the FDA for biomedical usage [15,22]. Remarkably, PEG can be used as a stabilizer during polymeric nanoparticle preparation [15] or applied as a linker for conjugation of targeting ligands to the surface of the nanoparticles [17]. Additionally, the modification of drug carriers with water-soluble PEG can contribute to a significant increase of the circulation half-life in vivo of asprepared nanovehicles [18].…”

Section: Classification and Properties Of Polymeric Nanoparticlesmentioning

confidence: 99%

“…Furthermore, polymer-based nanocarriers provide high thermodynamic stability to the system [15] and can easily permeate through various biological barriers [16]. It deserves to be emphasized that surface modification of polymeric nanoparticles can markedly increase the interaction of active substance with the biological target [17]. Intriguingly, the encapsulation or dispersion of drugs with a polymeric matrix might protect the drug molecules from premature release and degradation before reaching the targeted site [18].…”

mentioning

confidence: 99%

Recent Developments in Application of Polymeric Nanoparticles as Drug Carriers

Moritz¹,

Geszke-Moritz²

2015

Adv Clin Exp Med

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Nanotechnology is an interdisciplinary field of science offering interesting solutions for many branches of human life. Nanomaterials, defined as structures with at least one dimension below 100 nm, are the focus of increasing research attention as versatile tools for nanomedicine. Among the various nanostructures recently described in the literature, polymeric nanoparticles, characterized by satisfying biocompatibility, have aroused great interest as the carriers for various biologically active substances such as drugs, proteins and nucleic acids. These nanoparticles have already been reported as efficient vehicles for therapeutic agents in many disease entities. They can be delivered to the body via different administration routes. This review addresses recent advances in the usage of polymeric nanoparticles as drug carriers described in the years 2013 and 2014. The advantages of polymeric nanocarriers for medical application are highlighted, including their low toxicity, evaluated in vitro and in vivo. Moreover, the classification of polymeric nanoparticles is presented as well as various protocols of their synthesis (Adv Clin Exp Med 2015, 24, 5, 749-758).

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The effect of autophagy inhibitors on drug delivery using biodegradable polymer nanoparticles in cancer treatment

Cited by 159 publications

References 46 publications

Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Surface modification of MPEG-b-PCL-based nanoparticles via oxidative self-polymerization of dopamine for malignant melanoma therapy

Recent Developments in Application of Polymeric Nanoparticles as Drug Carriers

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