The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects

Handa, Tomoyuki; Anada, Takahisa; Honda, Yoshitomo; Yamazaki, Hiromi; Kobayashi, Kuriko; Kanda, Naoki; Kamakura, Shinji; Echigo, Seishi; Сузукі, Осаму

doi:10.1016/j.actbio.2011.12.002

Cited by 64 publications

(88 citation statements)

References 71 publications

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“…4,10 Briefly, the reagent grade of Gel isolated from porcine skin with an acidic process (Sigma-Aldrich, St. Louis, MO) was used. A calcium solution was mixed with a phosphate solution to attain supersaturation with respect to OCP to enhance the precipitation of OCP crystals in the presence of 0.5 wt% of Gel molecules for the total volume of the calcium and phosphate solutions.…”

Section: ) Preparation Of Implantsmentioning

confidence: 99%

“…Although the tissue-engineering technique by introducing exogenous cells into the OCP-based material certainly increases the osteogenic capability of the material, 9 the use of bone-substitute materials that have biological activity compatible with that of autologous bone, with the stimulation of host osteoblastic cells around OCP crystals, is still preferable from the viewpoint of reducing the complex handling and the cost of the preparation of an implant form. We recently succeeded in synthesizing such material 10 with bone regenerative capability approaching that of autologous bone to repair a critically sized rat calvaria defect composed of an OCP co-precipitated gelatin (Gel) composite obtained through OCP crystal growth in a solution containing Gel molecules. However, the effect of OCP/Gel composite on bone regeneration in long bone defect has never been examined.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, the bone regenerative capacity of an OCP/Gel composite containing 40 wt% of OCP was examined for a bone defect experimentally created in rabbit long bone. The OCP/Gel with 40 wt% of OCP is a composite that repairs most the intramembranous bone regeneration of the rat critical sized calvaria bone defect in a comparative study using various OCP/Gel composites containing 24 to 40 wt% of OCP 10) .…”

Section: Introductionmentioning

confidence: 99%

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Stimulatory Capacity of an Octacalcium Phosphate/Gelatin Composite on Bone Regeneration in a Rabbit Tibia Defect Model

Suzuki

Honda

Anada

et al. 2012

Phosphorus Research Bulletin

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The present study was designed to investigate whether an octacalcium phosphate/gelatin (OCP/Gel) composite can repair a defect created in rabbit tibia within 4 weeks. Histological and μCT studies showed that the implantation of OCP/Gel composite (40 wt% OCP) enhances cortical bone deposition over the defect accompanied by the formation of cartilage and bone marrow with cancellous bone within 2 weeks. The defects were repaired even in the control group (defect only) within 4 weeks; however, the repaired cortical bone was thinner than that in the OCP/Gel-implanted group. The results suggest that an OCP/Gel composite could be effectively used as a bone substitute material for autografts.

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Section: ) Preparation Of Implantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stimulatory Capacity of an Octacalcium Phosphate/Gelatin Composite on Bone Regeneration in a Rabbit Tibia Defect Model

Suzuki

Honda

Anada

et al. 2012

Phosphorus Research Bulletin

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“…Calcium phosphate bone fi lling materials, such as hydroxyapatite, octacalcium phosphate, and similar substances, are frequently used for the reconstruction of bone defects in dentistry and medicine [1][2][3][4] . However, if a powdered or granular bone substitute is filled, there is concern that it may easily f low out of the substituted site.…”

Section: Introductionmentioning

confidence: 99%

Dextrin Promotes Proliferation of Cultured MC3T3-E1 Mouse Osteoblast-like Cells and Their Alkaline Phosphatase Activity: Implications for Potential Application of Dextrin as a Binder of Bone Filling Material

Asai

Hayashi

Asakura

et al. 2018

J. Hard Tissue Biology.

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To develop a bone substitute with shape-generating properties, we investigated eff ects of dextrin, a low viscosity material, on MC3T3-E1 mouse osteoblast-like cells in vitro. At concentrations of 0.1 and 1.0 mM, dextrin promoted proliferative activity of MC3T3-E1 cells, whereas at concentration of 10 mM and higher, it negatively affected cell survival. In an alkaline phosphate (ALP) assay, MC3T3-E1 cells from experimental groups that were exposed to all tested concentrations of dextrin showed signifi cantly higher ALP activity values than cells in control group after day 9 in culture. In addition, blue-violet color in histochemical ALP staining experiments was detected in all groups on day 5 in culture; however, the most intensive staining was observed in MC3T3-E1 cells treated with 0.1 mM dextrin on day 11. Given to previously proven good biocompatibility of dextrin in vitro, our present results suggest that dextrin can be combined with bone fi lling material as a binder for clinical applications.

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“…Strategies to repair bone defects generally focus on preserving equivalent tissue engineering standards to heal CSDs caused by trauma or disease (Al Ruhaimi, 2001;Handa et al, 2012). Osteoconductive and osteo-inductive autografts are the gold standard for the clinical repair of bone defects.…”

Section: Introductionmentioning

confidence: 99%

Repair of critical-sized bone defects with anti-miR-31-expressing bone marrow stromal stem cells and poly(glycerol sebacate) scaffolds

Deng

Bi²,

Zhou³

et al. 2014

eCM

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The repair of critical-sized defects (CSDs) is a significant challenge in bone tissue engineering. Combining the use of progenitor cells with gene therapy represents a promising approach for bone regeneration. MicroRNAs play important roles in most gene regulatory networks, regulate the endogenous expression of multiple growth factors and simultaneously modulate stem cell differentiation. Our previous study showed that knocking down miR-31 promotes the osteogenesis of bone marrow stromal stem cells (BMSCs). To investigate the therapeutic potential of cells engineered to express anti-miR-31 for CSD repair, lentiviral vectors encoding negative control, miR-31 precursor and anti-sense sequences were constructed and transduced into osteo-inductive BMSCs. The expression of osteogenic-specific genes, alkaline phosphatase activity and Alizarin Red S staining were investigated to evaluate the effects of miR-31 on the cell fate of BMSCs over a 3-week period. In addition, miR-31-modified BMSCs seeded on poly(glycerol sebacate) (PGS) scaffolds were used to repair 8 mm critical-sized calvarial defects in rats. The results showed that miR-31 suppression significantly increased the expression of osteogenic-specific genes in vitro at the mRNA and protein levels, and that robust new bone formation with high local bone mineral density was observed in the anti-miR groups in vivo. Moreover, the PGS scaffolds carrying anti-miR-31-expressing BMSCs exhibited good biocompatibility and a high regeneration rate (~60 %) within in vivo bone defects. Our results suggest that miR-31 gene delivery affects the potential of BMSCs for osteogenic differentiation and bone regeneration and that PGS is a potential substrate for genetically modified, tissue-engineered bone in the repair of large bone defects.

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The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects

Cited by 64 publications

References 71 publications

Stimulatory Capacity of an Octacalcium Phosphate/Gelatin Composite on Bone Regeneration in a Rabbit Tibia Defect Model

Stimulatory Capacity of an Octacalcium Phosphate/Gelatin Composite on Bone Regeneration in a Rabbit Tibia Defect Model

Dextrin Promotes Proliferation of Cultured MC3T3-E1 Mouse Osteoblast-like Cells and Their Alkaline Phosphatase Activity: Implications for Potential Application of Dextrin as a Binder of Bone Filling Material

Repair of critical-sized bone defects with anti-miR-31-expressing bone marrow stromal stem cells and poly(glycerol sebacate) scaffolds

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