The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on callus growth and production flavonoid content on culture callus Acalypha indica L.

Rahayu, Bekti; Solichatun, Solichatun; Anggarwulan, Endang

doi:10.13057/biofar/f010101

Cited by 11 publications

(13 citation statements)

References 1 publication

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“…The use of different ZPT gives a variety of responses to each explant individual. This is due to the interaction between endogenous hormones in plant tissue and given exogenous growth regulators [13].Addition of 2.4 D in the media will stimulate cell division and enlargement in the explants so as to stimulate callus formation [14]. The addition of BAP into the media was able to play an active role in the growth and ploriferation of callus [15].…”

Section: Table 2 Percentage Of Callus Formation In Karamunting Explants With Treatment Of Several Concentrations Of 24d Bap and Tdzmentioning

confidence: 99%

Karamunting (Rhodomyrtus tomentosa) Callus Induction In Vitro

Rahmah¹,

Anwar²,

Swasti³

2020

IJEAB

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Karamunting plant is one of the biodiversity that must be developed because it has potential as phytopharmaca. The lack of public attention to the preservation and conservation of karamunting plants causes the scarcity of these plants, so it is necessary to do conservation in the form of propagation in vitro. One of the first steps that can be done is to get a callus induction protocol of karamunting plants. This study aim is to obtain a callus induction protocol for karamunting plants using 2,4D, TDZ and BAP growth regulators in vitro. This research was conducted in October to December 2019, at the Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang. This experiment was compiled based on a Completely Randomized Design (CRD).The treatment used was MS media with a combination of 2,4D, BAP, and Thidiazuron concentrations, namely: A = 2.5 ppm 2,4D, B = 5.0 ppm 2,4D, C = 2.5 ppm 2,4D + 1 ppm BAP, D = 5.0 ppm 2,2D + 1 ppm BAP, E = 2.5 ppm 2,4D + 2 ppm TDZ, F = 5.0 ppm 2,4D + 2 ppm TDZ. Explants in the form of karamunting leaves from seed germination in vitro. Based on the results of the study found the influence of growth regulators BAP, 2.4D and TDZ on the percentage of explants forming callus, callus texture and karamunting callus color. By administering 2.5 ppm 2,4D + 1 ppm BAP, 5.0 ppm 2,2D + 1 ppm BAP and 2.5 ppm 2,4D + 2 ppm TDZ are able to produce a 100% callus percentage. The 5.0 ppm 2,4D + 2 ppm TDZ treatment produced crumb callus with the highest percentage which was 90%, and 5.0 ppm 2,4D was able to produce compact callus with the highest percentage which was 100% and the 2.5 ppm 2,4D treatment, 2.5 ppm 2,4D and 5.0 ppm 2,4D + 2 ppm TDZ produce white callus with the highest percentage that is 100% and 2.5 ppm 2,4D treatment + 1 ppm BAP produces green callus with the most percentage which is 75%. While for the first time the callus appeared there was no effect of some concentrations of BAP, TDZ and 2,4D.

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Section: Table 2 Percentage Of Callus Formation In Karamunting Explants With Treatment Of Several Concentrations Of 24d Bap and Tdzmentioning

confidence: 99%

Karamunting (Rhodomyrtus tomentosa) Callus Induction In Vitro

Rahmah¹,

Anwar²,

Swasti³

2020

IJEAB

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“…Growth regulating agent kinetin works in the regulation of protein synthesis which can accelerate cell division to result in callus growth. The substance that regulates the growth of auxin or 2,4-D [26], works to spread the cell wall so that the hydrogen bond is released. The release of hydrogen bonds will cause the entry of water into the cell, the pressure of the turgor will rise which will cause cell enlargement…”

Section: E Identification Of Polyphenon From Camellia Sinensis L Callusmentioning

confidence: 99%

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Sutini¹,

Widiwurjani²,

Purwanto³

et al. 2018

Proceedings of the International Conference on Science and Technology (ICST 2018)

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The purpose of this study was to obtain a polyphenon profile from callus extract obtained from in vitro culture of Camellia sinensis L. Polyphenon is one of the bioactive compounds found in Camellia sinensis L plants which can also be produced through in vitro culture. Polyphenon as a bioactive is often used in various fields including in the fields of industry: manufacturing, food-drinks, health, agriculture, computers. To get a certain amount of polyphenon from in vitro culture, Camellia sinensis L callus was first extracted with water solvent, as well as non-water solvents / organic solvents such as ethyl alcohol. The methods used were: (1) inoculation of leaf explants from Camellia sinensis L in vitro on the media plus growth regulators to form callus, (2) treatment to be able to be reused (3) reap the callus followed by callus weighing and callus observation. (3) extraction and isolation of bioactive polyphenon (4) identification of polyphenon from Camellia sinensis L callus with high-performance liquid chromatography qualitatively and quantitatively. The results obtained are a form of polyphenon chromatogram profile from callus Camellia sinensis L which resembles a standard chromatogram of polyphenon.

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“…Perbedaaan kuantitas kalus terjadi karena eksplan tangkai daun yang digunakan memiliki kepekaan dan daya serap terhadap media yang berbeda serta adanya pengaruh dari zat pengatur tumbuh yang diberikan. Kecepatan sel dalam membelah akan mempengaruhi volume sel (Rahayu et al, 2003). Perlakuan terakhir yang belum menunjukkan adanya tanda-tanda berkalus adalah perlakuan B3N1.…”

Section: Kuantitas Kalus Umur 63 Hstunclassified