The effect of 1,25 dihydroxyvitamin D3 on HCl/Ethanol-induced gastric injury in rats

Şahin, Hasan; Cumbul, Alev; Uslu, Ünal; Yılmaz, Zeynep; Ercan, Feríha; Ali̇can, İnci̇

doi:10.1016/j.tice.2018.03.003

Cited by 12 publications

(6 citation statements)

References 48 publications

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“…It is well established that chronic alcohol consumption is one of the most important risk factors for the induction of digestive tract diseases ( 37 ). Chronic, excessive alcohol consumption may lead to the induction of various acute digestive tract diseases, including gastritis, gastric ulcers and gastrorrhagia, as a result of subsequent gastric mucosa injury, particularly via oxidative stress and apoptosis ( 38 , 39 ). Tea polyphenols, which are predominantly extracted from green tea, have been widely investigated and have been revealed to exhibit antioxidative properties ( 40 , 41 ).…”

Section: Discussionmentioning

confidence: 99%

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

2018

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Ethanol-induced diseases of the gastric mucosa are the most common and refractory diseases of gastrointestinal system in clinic, and are mediated by oxidative stress and apoptosis pathways. Theaflavins (TFs) are considered to be antioxidants. The present study aimed to determine the molecular mechanism underlying the ability of TFs to attenuate ethanol-induced oxidative stress and apoptosis in GES-1 gastric mucosa epithelial cells. A Cell Counting Kit-8 (CCK-8) assay was performed to investigate the cell viability of GES-1 cells following administration of ethanol (0.5 mol/l) and subsequent treatment with TFs (20, 40 and 80 µg/ml) for specific time intervals. A carboxyfluorescein diacetate succinimidyl ester assay was used to measure proliferation and further investigate the results of the CCK-8 assay. Flow cytometry was performed to measure reactive oxygen species (ROS) levels and the apoptosis rates of GES-1 cells. Furthermore, levels of oxidative stress-associated factors, including malondialdehyde, superoxide dismutase and glutathione, were investigated using commercial kits. Reverse transcription-quantitative polymerase chain reaction and western blot assays were performed to determine the expression levels of apoptosis-associated factors, as well as the phosphorylation levels of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38). The results of the present study demonstrated that treatment with ethanol inhibited GES-1 cell proliferation, and enhanced ROS levels and apoptosis rates, potentially via downregulation of B-cell lymphoma-2 (Bcl-2) expression and upregulation of Bcl-2-associated X and caspase-3 expression levels, as well as enhancing the phosphorylation levels of ERK, JNK and p38. However, treatment with TFs was revealed to attenuate the effects of ethanol administration on GES-1 cells in a dose-dependent manner. In conclusion, TFs may attenuate ethanol-induced oxidative stress and apoptosis in gastric mucosa epithelial cells via downregulation of various mitogen-activated protein kinase pathways.

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Section: Discussionmentioning

confidence: 99%

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

2018

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“…Gastric ulcers are caused by a disruption in the homeostasis between the acidic and protective environment of the gastric mucosa [161,184]. The ulceroprotective and antioxidant effects of 1α,25(OH) 2 D3 were reported in rats [149]. H. pylori gastritis infection, which is the Th1 type, causes autoimmune gastritis (AIG), and is classified as an autoimmune disease.…”

Section: Vitamin D and The Gastric Tissuementioning

confidence: 99%

A Narrative Role of Vitamin D and Its Receptor: With Current Evidence on the Gastric Tissues

Sirajudeen

Shah

Menhali

2019

IJMS

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Vitamin D is a major steroid hormone that is gaining attention as a therapeutic molecule. Due to the general awareness of its importance for the overall well-being, vitamin D deficiency (VDD) is now recognized as a major health issue. The main reason for VDD is minimal exposure to sunlight. The vitamin D receptor (VDR) is a member of the steroid hormone receptors that induces a cascade of cell signaling to maintain healthy Ca2+ levels that serve to regulate several biological functions. However, the roles of vitamin D and its metabolism in maintaining gastric homeostasis have not yet been completely elucidated. Currently, there is a need to increase the vitamin D status in individuals worldwide as it has been shown to improve musculoskeletal health and reduce the risk of chronic illnesses, including some cancers, autoimmune and infectious diseases, type 2 diabetes mellitus, neurocognitive disorders, and general mortality. The role of vitamin D in gastric homeostasis is crucial and unexplored. This review attempts to elucidate the central role of vitamin D in preserving and maintaining the overall health and homeostasis of the stomach tissue.

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“…The mechanism of ethanol-induced injury is complex and not fully understood [5]. However, HCl/ethanol causes hemorrhagic lesions, edema, epithelial exfoliation, extravasation of neutrophils, which are similar to lesions that result from alcohol abuse [6].…”

Section: Introductionmentioning

confidence: 99%

Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms

et al. 2019

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Gastric ulcer is sores that form in the stomach mucosal layer because of erosion caused by high acid secretion and excessive use of non-steroidal anti-inflammatory drugs. Prodigiosins (PdGs) are red-pigmented secondary metabolites produced by bacteria, including actinomycetes. Butylcycloheptylprodigiosin ( 1 ) and undecylprodigiosin ( 2 ) were identified and isolated from a crude extract of the actinomycete RA2 isolated from the Red Sea Sponge Spheciospongia mastoidea . Chemical structure of 1 and 2 was determined by NMR and mass spectroscopy. Although their antioxidant and anti-inflammatory properties are known, their effect on gastric lesion is unknown. Therefore, this study aimed to investigate gastroprotective effects of PdGs against HCl/ethanol-induced gastric lesion in rats. Oral pretreatment with PdGs (100, 200, and 300 mg/kg) attenuated severity of HCl/ethanol-induced gastric mucosal injury, as evidenced by decreases in gastric lesion index scores, ulceration area, histopathologic abnormality, and neutrophil infiltration. These effects were comparable to those of omeprazole, a standard anti-gastric ulcer agent. HCl/ethanol-induced gastric erosions was associated with tremendous increases in lipid peroxidation, nitric oxide, and pro-inflammatory cytokines and mediators (myeloperoxidase, interleukin-1β, tumor necrosis factor-α, and cyclooxygenase-2), and with significant decreases in enzymatic and non-enzymatic antioxidant activities. However, PdGs ameliorated gastric inflammation and oxidative stress by downregulating nuclear factor kappa B and inducible nitric oxide synthase expression and upregulating heme oxygenase-1 expression. PdGs prevented gastric mucosal apoptosis by downregulating Bax and caspase-3 expression and upregulating Bcl-2 expression, thereby increasing prostaglandin E2 production. Our results suggested that PdGs exerted gastroprotective effects by decreasing the levels of inflammatory mediators, apoptotic markers, and antioxidants.

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The effect of 1,25 dihydroxyvitamin D3 on HCl/Ethanol-induced gastric injury in rats

Cited by 12 publications

References 48 publications

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

Theaflavins attenuate ethanol‑induced oxidative stress and cell apoptosis in gastric mucosa epithelial cells via downregulation of the mitogen‑activated protein kinase pathway

A Narrative Role of Vitamin D and Its Receptor: With Current Evidence on the Gastric Tissues

Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms

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