The early UL3 gene of equine herpesvirus-1 encodes a tegument protein not essential for replication or virulence in the mouse

Ahn, Byung Chul; Kim, Seong‐Man; Zhang, Yunfei; Charvat, Robert A.; O’Callaghan, Dennis J.

doi:10.1016/j.virol.2011.08.016

Cited by 10 publications

(5 citation statements)

References 53 publications

(61 reference statements)

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“…Circ is a small, myristylated protein non-essential for replication in tissue culture [ 55 ] that localizes to the virion [ 56 ]. The homolog protein in equine herpesvirus-1 (EHV-1), UL1, also localizes in the tegument [ 57 ], in contrast to ORF-2 of varicella zoster virus (VZV) which does not [ 58 ]. Circ's function is not clear.…”

Section: Discussionmentioning

confidence: 99%

Protein Composition of the Bovine Herpesvirus 1.1 Virion

Barber

Daugherty

Ander

et al. 2017

Veterinary Sciences

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Bovine herpesvirus (BoHV) type 1 is an important agricultural pathogen that infects cattle and other ruminants worldwide. Acute infection of the oro-respiratory tract leads to immune suppression and allows commensal bacteria to infect an otherwise healthy lower respiratory tract. This condition is known as the Bovine Respiratory Disease (BRD). BoHV-1 latently infects the host for life and periodical stress events re-initiate BRD, translating into high morbidity and large economic losses. To gain a better understanding of the biology of BoHV-1 and the disease it causes, we elucidated the protein composition of extracellular virions using liquid chromatography-mass spectrometry analysis. We detected 33 viral proteins, including the expected proteins of the nucleocapsid and envelope as well as other regulatory proteins present in the viral tegument. In addition to viral proteins, we have also identified packaged proteins of host origin. This constitutes the first proteomic characterization of the BoHV virion.

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Section: Discussionmentioning

confidence: 99%

Protein Composition of the Bovine Herpesvirus 1.1 Virion

Barber

Daugherty

Ander

et al. 2017

Veterinary Sciences

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“… b Function is assigned primarily from published data for proteins of herpes simplex virus 1 (HSV-1) ( 23 , 43 , 44 ). The discovery and function of EHV-1 IR2 ( 25 , 26 ), IR3 ( 27 – 29 ), UL1 and UL2 ( 39 ), UL3 ( 45 ), UL11 ( 46 ), UL20 ( 47 ), UL31 ( 48 ), UL34 ( 49 ), US4 (gp2) ( 50 ), and US6 (gI) and US7 (gE) ( 37 ) were reported previously . –, not present .…”

Section: Resultsmentioning

confidence: 55%

Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11

2017

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Equine herpesvirus 1 (EHV-1) is a major pathogen affecting equines worldwide. The virus causes respiratory disease, abortion, and, in some cases, neurological disease. EHV-1 Kentucky A (KyA) is attenuated in the mouse and equine, whereas wild-type pathogenic strain RacL11 induces severe inflammatory infiltration of the lung, causing infected mice to succumb. The complete DNA sequencing of the KyA genome revealed that genes UL17 (ORF17), US6 (ORF73; gI), US7 (ORF74; gE), and US8 (ORF75; 10 K) are deleted as compared to the RacL11 and Ab4 genomes. In-frame deletions in the US1 (ORF68), US4 (ORF71; gp2), and UL63 (ORF63; EICP0) genes and point mutations in 14 different open reading frames (ORFs) were detected in the KyA genome. Interestingly, UL1 (ORF1) and UL2 (ORF2) were deleted in both KyA and RacL11. Our previous studies showed that EHV-1 glycoproteins gI, gE, and full-length gp2 contribute to the pathogenesis of the RacL11 strain. The confirmation of these gene deletions in KyA suggests their contribution to the attenuation of this virus. The growth kinetics results revealed that KyA replicates to high titers in cell culture as compared to RacL11 and Ab4, indicating that the above genomic deletions and mutations in KyA do not have an inhibitory effect on KyA replication in cells of mouse, rabbit, equine, or human origin. Studies of EHV-1 pathogenesis in CBA mice showed that KyA is attenuated whereas mice infected with RacL11 succumbed by 3–6 days post-infection, which is consistent with our previous results.

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“…We recently observed that EHV-1 early regulatory proteins IR2P, UL3P, and UL4P act as the negative regulatory protein for EHV-1 gene expression (Ahn et al, 2011; Charvat et al, 2011; Kim et al, 2006, 2011). Unlike the IEP, these negative regulatory proteins have a broad inhibitory function that may prevent transcription of a variety of EHV-1 promoters by interfering with the interaction between the GTFs.…”

Section: Discussionmentioning

confidence: 99%

Characterization of cis-acting elements required for autorepression of the equine herpesvirus 1 IE gene

et al. 2012

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The immediate-early protein (IEP), the major regulatory protein encoded by the IE gene of equine herpesvirus 1 (EHV-1), plays a crucial role as both transcription activator and repressor during a productive lytic infection. To investigate the mechanism by which the EHV-1 IEP inhibits its own promoter, IE promoter-luciferase reporter plasmids containing wild-type and mutant IEP-binding site (IEBS) were constructed and used for luciferase reporter assays. The IEP inhibited transcription from its own promoter in the presence of a consensus IEBS (5’-ATCGT-3’) located near the transcription initiation site but did not inhibit when the consensus sequence was deleted. To determine whether the distance between the TATA box and the IEBS affects transcriptional repression, the IEBS was displaced from the original site by the insertion of synthetic DNA sequences. Luciferase reporter assays revealed that the IEP is able to repress its own promoter when the IEBS is located within 26-bp from the TATA box. We also found that the proper orientation and position of the IEBS were required for the repression by the IEP. Interestingly, the level of repression was significantly reduced when a consensus TATA sequence was deleted from the promoter region, indicating that the IEP efficiently inhibits its own promoter in a TATA box-dependent manner. Taken together, these results suggest that the EHV-1 IEP delicately modulates autoregulation of its gene through the consensus IEBS that is near the transcription initiation site and the TATA box.

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The early UL3 gene of equine herpesvirus-1 encodes a tegument protein not essential for replication or virulence in the mouse

Cited by 10 publications

References 53 publications

Protein Composition of the Bovine Herpesvirus 1.1 Virion

Protein Composition of the Bovine Herpesvirus 1.1 Virion

Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11

Characterization of cis-acting elements required for autorepression of the equine herpesvirus 1 IE gene

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