The distribution of immune cells and macrophages in the endometrium of women with recurrent reproductive failure. III: Further observations and reference ranges

Russell, Peter; Sacks, Gavin; Tremellen, Kelton; Gee, Alison

doi:10.1097/pat.0b013e328361429b

Cited by 76 publications

(57 citation statements)

References 28 publications

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“…The majority of research to date on NK cell function in the reproductive tract focuses on their role during embryo implantation and placentation within the uterus [44]. It is important to mention that the majority of primate uterine NK cells are CD56 bright , and increased numbers of CD56 dim NK cells in the uterus are associated with reproductive failure [45]. Notably, a subpopulation of primate NK cells is CD16 + CD56 dim [46].…”

Section: Discussionmentioning

confidence: 98%

Dynamics of Immune Cell Types Within the Macaque Corpus Luteum During the Menstrual Cycle: Role of Progesterone1

Bishop

Molskness

et al. 2015

Biology of Reproduction

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The goal of the current study was to characterize the immune cell types within the primate corpus luteum (CL). Luteal tissue was collected from rhesus females at discrete intervals during the luteal phase of the natural menstrual cycle. Dispersed cells were incubated with fluorescently labeled antibodies specific for the immune cell surface proteins CD11b (neutrophils and monocytes/macrophages), CD14 (monocytes/macrophages), CD16 (natural killer [NK] cells), CD20 (B-lymphocytes), and CD3epsilon (T-lymphocytes) for analysis by flow cytometry. Numbers of CD11b-positive (CD11b(+)) and CD14(+) cells increased significantly 3 to 4 days after serum progesterone (P4) concentrations declined below 0.3 ng/ml. CD16(+) cells were the most abundant immune cell type in CL during the mid and mid-late luteal phases and were 3-fold increased 3 to 4 days after serum P4 decreased to baseline levels. CD3epsilon(+) cells tended to increase 3 to 4 days after P4 decline. To determine whether immune cells were upregulated by the loss of luteotropic (LH) support or through loss of LH-dependent steroid milieu, monkeys were assigned to 4 groups: control (no treatment), the GnRH antagonist Antide, Antide plus synthetic progestin (R5020), or Antide plus the estrogen receptor agonists diarylpropionitrile (DPN)/propyl-pyrazole-triol (PPT) during the mid-late luteal phase. Antide treatment increased the numbers of CD11b(+) and CD14(+) cells, whereas progestin, but not estrogen, replacement suppressed the numbers of CD11b(+), CD14(+), and CD16(+) cells. Neither Antide nor steroid replacement altered numbers of CD3epsilon(+) cells. These data suggest that increased numbers of innate immune cells in primate CL after P4 synthesis declines play a role in onset of structural regression of primate CL.

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Section: Discussionmentioning

confidence: 98%

Dynamics of Immune Cell Types Within the Macaque Corpus Luteum During the Menstrual Cycle: Role of Progesterone1

Bishop

Molskness

et al. 2015

Biology of Reproduction

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“…Although they were initially reported as absent in proliferative endometrium based on histochemical stains to detect cytoplasmic granules, CD56+ cells are detectable, albeit in relatively low numbers . The increase in numbers is seen from day 22 onwards (Russell et al 2011(Russell et al , 2013, and at least some of the increase can be accounted for by local proliferation. The phenotype of uNK cells in non-pregnant endometrium has not been investigated to the same extent as those from early pregnancy decidua, where distinct gestational age differences in phenotype and function have been reported.…”

Section: Relationship Of Unk Cells In Non-pregnant Endometrium and Dementioning

confidence: 95%

“…between zona spongiosum and zona compactum), an initial step should be to establish consistent methodology for quantifi cation of uNK cell numbers in endometrium (Lash et al 2014 ). Furthermore, the date of assessment may vary from LH+6 to LH+9 in different studies, refl ecting a cycle stage when uNK cell numbers can vary dramatically (Russell et al 2011(Russell et al , 2013. It is essential that sampling is on a specifi c day of the cycle or that 'normal' ranges for uNK cell numbers are established in large subject groups.…”

Section: Clinical Applications Of Unk Assessment In Recurrent Reprodumentioning

confidence: 99%

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

Bulmer

Lash

2015

Advances in Experimental Medicine and Biology

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The human endometrium contains a substantial population of leucocytes which vary in distribution during the menstrual cycle and pregnancy. An unusual population of natural killer (NK) cells, termed uterine NK (uNK) cells, are the most abundant of these cells in early pregnancy. The increase in number of uNK cells in the mid-secretory phase of the cycle with further increases in early pregnancy has focused attention on the role of uNK cells in early pregnancy. Despite many studies, the in vivo role of these cells is uncertain. This chapter reviews current information regarding the role of uNK cells in healthy human pregnancy and evidence indicating their importance in various reproductive and pregnancy problems. Studies in humans are limited by the availability of suitable tissues and the limitations of extrapolation from animal models.

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“…Numbers of uNK cells increase exponentially as the menstrual cycle progresses (Bulmer & Lash 2005;Russell et al 2011;Russell et al 2013) and therefore timing ofthe biopsy is critical or a reference range of 'normal' uNK cell numbers throughout the menstrual cycle needs to be established. However, before this can be addressed a method which provides consistency between different centres is required and this study has addressed this.…”

Section: Discussionmentioning

confidence: 99%

“…There is also no clear definition of a "normal range" of uNK cell numbers, partly because obtaining endometrium from normal fertile control women is difficult. In addition, for practical reasons many centres sample over a 3 day period (LH+6-LH+8 or LH+7-LH+9), but during this time 100 period uNK cell numbers increase exponentially (Russell et al 2011;Russell et al 2013), which may skew clinical results dependent on the "reference range" being used.…”

mentioning

confidence: 99%

Standardisation of uterine natural killer (uNK) cell measurements in the endometrium of women with recurrent reproductive failure

Lash

Bulmer

et al. 2016

Journal of Reproductive Immunology

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The distribution of immune cells and macrophages in the endometrium of women with recurrent reproductive failure. III: Further observations and reference ranges

Cited by 76 publications

References 28 publications

Dynamics of Immune Cell Types Within the Macaque Corpus Luteum During the Menstrual Cycle: Role of Progesterone1

Dynamics of Immune Cell Types Within the Macaque Corpus Luteum During the Menstrual Cycle: Role of Progesterone1

The Role of Uterine NK Cells in Normal Reproduction and Reproductive Disorders

Standardisation of uterine natural killer (uNK) cell measurements in the endometrium of women with recurrent reproductive failure

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