The Diploid-Like Cytological Behavior of Tetraploid Cotton

Endrizzi, J. E.

doi:10.1111/j.1558-5646.1962.tb03224.x

Cited by 36 publications

(23 citation statements)

References 13 publications

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“…The formation of trivalents and higher chromosome associations in triploids and hexaploids indicate the pairing affinities between the genome involved. Endrizzi (1962) reported that the main force, which controls regular pairing behaviour in Gossypium, is the differences in the degree of chromosome condensation. Homeologous chromosome are differently sized among Gossypium genomes which rarely pair.…”

Section: Discussionmentioning

confidence: 99%

Production and Fertility Restoration of an Interspecific Hybrid between Gossypium hirsutum L. and G. raimondii U.

et al. 2007

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Summary Interspecific hybrids between 2 cultivated varieties (MCU 5 and MCU 7) of tetraploid cotton (Gossypium hirsutum) and diploid perennial species G. raimondii were synthesized. The resulting triploid F 1 s were pollen sterile and exhibited irregular meiosis with frequent formation of univalents and multivalents. Mean chromosome association of the triploid hybrids were 11.50 Iϩ9.97 IIϩ2.31 IIIϩ0.16 IV and 0.50 Iϩ11.35 IIϩ0.96 I. Successful synthesis of hexaploids involoving G. raimondii by doubling the genomes of the triploid hybrids with 0.1% colchicine and enhancement of fertility status in hybrids is reported for the first time. The mean chromosomal association of hexaploid hybrids were 4.05 Iϩ26.91 IIϩ4.45 IIIϩ1.23 IVϩ0.32 V and 1.26 Iϩ25.81 IIϩ1.18 IIIϩ5.19 IV respectively. The heterozygosity of triploid and hexaploid hybrids was also confirmed through SSR marker analysis. Further utility of the developed hybrids with respect to jassid resistance breeding in cotton is discussed.

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Section: Discussionmentioning

confidence: 99%

Production and Fertility Restoration of an Interspecific Hybrid between Gossypium hirsutum L. and G. raimondii U.

et al. 2007

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“…Bivalents observed in the hybrid may have formed by both homologous and homeologous pairing a result of different affinity between intergenome chromosome in Gossypium spp. (Endrizzi 1962).…”

Section: Scoring and Data Analysismentioning

confidence: 99%

RAPD and Cytogenetic Study in F1 and F2 of Interspecific Cross between Gossypium arboreum*Gossypium anomalum

et al. 2008

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Summary An interspecific hybrid between G. arboreumϫG. anomalum was obtained by pollinating previous day bagged flowers of G. arboreum genetic male sterile line MPKV GMS with pollen from wild G. anomalum.The chromosome constitution in all the plants of G. arboreum and G. anomalum were found to be normal while interspecific hybrid showed mean chromosome association 1.081 I ϩ10.479 II ϩ0.000 III ϩ0.990 IV with normal sporads. Interspecific hybrid showed anomalous behavior of univalent and formation of single and double chromatin bridges both during fist and second meiotic division of meiosis. Meiotic chromosome association was observed in nine plants of F 2 generation. The cytological studies showed that F 1 and F 2 generations were having irregular pairing and unequal separation of chromosomes. This led to pollen sterility in these generations. In F 2 plants some good plants identified with higher pollen fertility and more number of bolls per plant. Selections from these plants can help to introgress desired characters from G. anomalum to G. arboreum. Random Amplified Polymorphic DNA (RAPD) analysis was employed to characterize the F 1 interspecific hybrid of G. arboreum MPKV GMSϫG. anomalum and its nine segregants. A total fifteen primers of 10 mer were screened of which eight were selected based on polymorphism of amplified fragments, amplification intensity and the presence of informative markers. Maximum 98 amplified fragments were resolved on the gel for parents, F 1 and F 2 's with OPC-19 primers, whereas, minimum 25 fragments were seen with OPD-04 primer. Similarity coefficient based on DNA amplification using amplified RAPD primer was estimated. The values for genetic similarity ranged from 0.14 to 0.77.

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“…Cytological study helps in establishing desired forms in a more precise way within a shorter span of time. Endrizzi et al (1985) reported that the chromosomes of A and B genomes are intermediate in size between small chromosomes of D genomes and the large chromosomes of C, E, F, G and K genomes.…”

mentioning

confidence: 99%

“…The formation of trivalents and higher chromosome associations indicate the pairing affinities between the genomes involved. Endrizzi (1962) reported that the main force which controls regular pairing behaviour in Gossypium to be the difference in the degree of chromosome condensation. Homologous chromosome of differently sized Gossypium genomes rarely pair.…”

mentioning

confidence: 99%

Production of Interspecific Hybrids between Gossypium hirsutum and Jassid Resistant Wild Species G. raimondii and G. armourianum

Pushpam

Raveendran

2006

CYTOLOGIA

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Summary Present study attempts to explore the possibility of successfully transferring the jassid resistant genes from 2 wild cotton species G. armourianum (Kearney. (2nϭ2xϭ26) D2A) and G. raimondii (Ulbrich. (2nϭ2xϭ26) D5) into the cultivated G. hirsutum genotypes through backcrossing and colchiploidy. The investigation revealed that viable seeds of the crosses between G. hirsutum and wild diploid species G. armourianum and G. raimondii were obtained using G. hirsutum as female. The backcrosses using the triploid (F 1 ), as female and tetraploid hirsutum as males was not successful, the reciprocal yielded few seeds. Of 432 F 1 plants treated with colchicine, only 2 plants each in the crosses viz., SurabhiϫG. raimondii and MCU 9ϫG. armourianum were polyploidised. The colchiploids C 1 (SurabhiϫG. raimondii) showed larger leaves, flowers, petal size, petal spot, thick leaves, anther density and increased pollen fertility, whereas C 1 (MCU 9ϫG. armourianum) showed stunted growth, hard stem and thick leaves. Leaf hairiness, leaf texture and leaf shape of the BC 1 plants [C 1 (SurabhiϫG. raimondii)ϫSurabhi] resembled its donor parent. This indicates that the hairiness, which is an important character conferring jassid resistance, has descended from the wild parent G. raimondii. The BC 1 plants of C 1 (MCU 9ϫG. armourianum) had leathery leaves and deterring citronella like odour contributing to jassid resistance is derived from the wild species G. armourianum. The result of cytological investigations also supported the crossability behaviour as a close homology between chromosomes of G. armourianum, G. raimondii and G. hirsutum. In the present study, greater homology observed between A and D genomes aids in production of desirable recombinants despite minor cytological disturbances, as there are successful boll setting and viable seed production. Hence, these species with D genome can be used successfully in gene transfer if fertilization barriers are overcome by novel techniques.

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The Diploid-Like Cytological Behavior of Tetraploid Cotton

Cited by 36 publications

References 13 publications

Production and Fertility Restoration of an Interspecific Hybrid between Gossypium hirsutum L. and G. raimondii U.

Production and Fertility Restoration of an Interspecific Hybrid between Gossypium hirsutum L. and G. raimondii U.

RAPD and Cytogenetic Study in F1 and F2 of Interspecific Cross between Gossypium arboreum*Gossypium anomalum

Production of Interspecific Hybrids between Gossypium hirsutum and Jassid Resistant Wild Species G. raimondii and G. armourianum

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