The differential diagnostic model for serous peptidomics in HBV carriers established by MALDI-TOF-MS analysis

Tian, Liyuan; Wang, Yu; Xu, Dabin; Gao, Yanhong; Wen, Xin; Tian, Yaping

doi:10.1016/j.clinbiochem.2013.10.016

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…This finding was consistent with a published report, which showed that the level of FGB was significantly decreased in the plasma of 116 HBV-infected individuals by western blot analysis, compared with 70 normal subjects [22]. …”

Section: Discussionsupporting

confidence: 93%

LHBs can elevate the expression of MDR1 through HIF-1α in patients with CHB infection: a comparative proteomic study

Yang

Ding

et al. 2016

Oncotarget

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Background and Aims: Hepatitis B virus (HBV) infection is a major risk factor for liver cirrhosis and hepatocellular carcinoma (HCC). To gain a better understanding of the pathogenesis of HBV infection, this study aimed to investigate the differentially expressed proteins (DEPs) in liver tissues from patients with chronic hepatitis B (CHB) infection.Results: Seventy-one DEPs were identified. Overexpression of multi-drug

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Section: Discussionsupporting

confidence: 93%

LHBs can elevate the expression of MDR1 through HIF-1α in patients with CHB infection: a comparative proteomic study

Yang

Ding

et al. 2016

Oncotarget

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“…[253][254][255] In turn, Fourier transform-ion cyclotron resonance-MS is more suitable for targeted/confirmatory analysis when dealing with fewer peptides. 256 Because these apparatuses allow tandem MS to be performed, peptides can be readily sequenced in addition to their identification, circumventing the above-mentioned limitations concerning immunological assays and largely simplifying peptidomics workflows.…”

Section: Peptidomics Of Native Peptides In Human Bodily Fluidsmentioning

confidence: 99%

“…From this point onwards, almost any mass analyzer can be used, although in practice quadrupole time‐of‐flight (Q‐TOF) and ion traps (IT) instruments are the most frequently utilized ones in peptidomics, especially in exploratory studies . In turn, Fourier transform‐ion cyclotron resonance‐MS is more suitable for targeted/confirmatory analysis when dealing with fewer peptides . Because these apparatuses allow tandem MS to be performed, peptides can be readily sequenced in addition to their identification, circumventing the above‐mentioned limitations concerning immunological assays and largely simplifying peptidomics workflows.…”

Section: Peptidomics Of Native Peptides In Human Bodily Fluidsmentioning

confidence: 99%

Human Antimicrobial Peptides in Bodily Fluids: Current Knowledge and Therapeutic Perspectives in the Postantibiotic Era

Bastos

Trindade

Costa

et al. 2017

Medicinal Research Reviews

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Antimicrobial peptides (AMPs) are an integral part of the innate immune defense mechanism of many organisms. Due to the alarming increase of resistance to antimicrobial therapeutics, a growing interest in alternative antimicrobial agents has led to the exploitation of AMPs, both synthetic and isolated from natural sources. Thus, many peptide-based drugs have been the focus of increasing attention by many researchers not only in identifying novel AMPs, but in defining mechanisms of antimicrobial peptide activity as well. Herein, we review the available strategies for the identification of AMPs in human body fluids and their mechanism(s) of action. In addition, an overview of the distribution of AMPs across different human body fluids is provided, as well as its relation with microorganisms and infectious conditions.

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“…Fibrinogen, which participates in the process of blood clotting, is synthesized in the liver. Many previous studies had shown that fragments of fibrinogen alpha or beta chains were associated with liver tumors [21,24,26,27]. ITIH4, an acute-phase glycoprotein produced primarily in the liver, is involved in liver development and stabilization of the extracellular matrix (ECM), and its expression is altered in liver disease [28].…”

Section: Discussionmentioning

confidence: 99%

Application Value of Mass Spectrometry in the Differentiation of Benign and Malignant Liver Tumors

Guo

et al. 2017

Med Sci Monit

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BackgroundDifferentiation of malignant from benign liver tumors remains a challenging problem. In recent years, mass spectrometry (MS) technique has emerged as a promising strategy to diagnose a wide range of malignant tumors. The purpose of this study was to establish classification models to distinguish benign and malignant liver tumors and identify the liver cancer-specific peptides by mass spectrometry.Material/MethodsIn our study, serum samples from 43 patients with malignant liver tumors and 52 patients with benign liver tumors were treated with weak cation-exchange chromatography Magnetic Beads (MB-WCX) kits and analyzed by the Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry (MALDI-TOF-MS). Then we established genetic algorithm (GA), supervised neural networks (SNN), and quick classifier (QC) models to distinguish malignant from benign liver tumors. To confirm the clinical applicability of the established models, the blinded validation test was performed in 50 clinical serum samples. Discriminatory peaks associated with malignant liver tumors were subsequently identified by a qTOF Synapt G2-S system.ResultsA total of 27 discriminant peaks (p<0.05) in mass spectra of serum samples were found by ClinPro Tools software. Recognition capabilities of the established models were 100% (GA), 89.38% (SNN), and 80.84% (QC); cross-validation rates were 81.67% (GA), 81.11% (SNN), and 86.11% (QC). The accuracy rates of the blinded validation test were 78% (GA), 84% (SNN), and 84% (QC). From the 27 discriminatory peptide peaks analyzed, 3 peaks of m/z 2860.34, 2881.54, and 3155.67 were identified as a fragment of fibrinogen alpha chain, fibrinogen beta chain, and inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), respectively.ConclusionsOur results demonstrated that MS technique can be helpful in differentiation of benign and malignant liver tumors. Fibrinogen and ITIH4 might be used as biomarkers for the diagnosis of malignant liver tumors.

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The differential diagnostic model for serous peptidomics in HBV carriers established by MALDI-TOF-MS analysis

Cited by 11 publications

References 35 publications

LHBs can elevate the expression of MDR1 through HIF-1α in patients with CHB infection: a comparative proteomic study

LHBs can elevate the expression of MDR1 through HIF-1α in patients with CHB infection: a comparative proteomic study

Human Antimicrobial Peptides in Bodily Fluids: Current Knowledge and Therapeutic Perspectives in the Postantibiotic Era

Application Value of Mass Spectrometry in the Differentiation of Benign and Malignant Liver Tumors

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