The diagnostic value of morphometry on blood lymphocytes in erythrodermic actinic reticuloid

Preesman, A. H.

doi:10.1001/archderm.131.11.1298

Cited by 7 publications

(5 citation statements)

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“…In our cohort we could detect > 20% Sézary cells within the total lymphocyte population by morphology in 13 of 17 patients. The specificity of detecting Sézary cells is further decreased by the observation of cells with cerebriform nuclei in the peripheral blood of people with various benign inflammatory conditions and normal individuals albeit in much smaller numbers than in SS 23–25 . Therefore, it is likely that the Sézary count reflects the presence of some proportion of both activated normal lymphocytes that tend to be small in size and neoplastic cells with diameters that potentially range from small to very large 25–27 .…”

Section: Discussionmentioning

confidence: 99%

The diagnosis of Sézary syndrome on peripheral blood by flow cytometry requires the use of multiple markers

Klemke

Brade

Weckesser

et al. 2008

British Journal of Dermatology

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Section: Discussionmentioning

confidence: 99%

The diagnosis of Sézary syndrome on peripheral blood by flow cytometry requires the use of multiple markers

Klemke

Brade

Weckesser

et al. 2008

British Journal of Dermatology

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“…This feature was used in quantitative analysis. Nuclear contour index (NCI) became a standard parameter for their recognition [15][16][17][18]. NCI is defined as the perimeter divided by the square root of the area.…”

Section: Discussionmentioning

confidence: 99%

“…It is thus analogous to the standard shape factor used in the present investigation, in fact SF = 4/NCI 2 . Using this single parameter, Preesman et al [17] were able to discriminate the circulating Sézary cells and used this method for distinguishing actinic reticulosis from Sézary syndrome. On the other hand Meijer et al [16] failed to recognize 1/3 of mycosis fungoides cases by NCI only; using multiparameter approach allowed them to recognize all the malignant cases properly.…”

Section: Discussionmentioning

confidence: 99%

Karyometric Comparison of Splenic and Gastric Marginal Zone Lymphomas

Styczeń

Szpor

Demczuk

et al. 2012

Analytical Cellular Pathology

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Background: Marginal zone lymphomas are indolent B-cell lymphomas associated with autoimmunity and chronic inflammation. The two most frequent variants are mucosa associated lymphoid tissues marginal zone lymphomas and splenic marginal zone lymphomas. The aim of the study was to determine if it is possible to classify splenic and gastric lymphomas according to karyometric features. Methods: The material consisted of 16 splenic and 14 gastric lymphomas. The measurements were done with the AnalySIS image analysis system. In each case at least 100 nuclei were selected, and 19 different geometric parameters were measured. Results: On statistical analysis, the nuclei of splenic and gastric lymphomas showed differences in most parameters, but significant overlap of the values was present. Neural networks were trained and used for classification of the data. By this method, the nuclei were properly classified with a sensitivity of 0.75 and specificity of 0.71. In addition, in all the cases the majority of the nuclei were properly classified, thus allowing correct classification of all the cases into “splenic” or “gastric”. Conclusion: These results support the view that mucosa-associated lymphoid tissue lymphomas and splenic marginal-zone lymphomas are separate entities.

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“…SØzary-Zellen von atypischen reaktiven T-Zellen im Blut beim kutanen T-Zell-Lymphom zu ermöglichen, wurden bisher verschiedenste Eigenschaften untersucht. Jedoch lassen sich weder morphologische [40], immunphänotypische [41 ± 43], ultrastrukturelle [44,45] Merkmale finden, die zuverlässig diese wichtige Unterscheidung ermöglichen. Hier erbrachte auch die Analyse der Vb-Genexpression der T-Zell-Rezeptoren auf Proteinebene [46,47] sowie der Nachweis chromosomaler Aberrationen [48,49] bisher keine für die Routine praktischen Fortschritte.…”

Section: Bedeutung Der Klonalität In Der Ausbreitungsdiagnostikunclassified

Diagnostischer Stellenwert und klinische Bedeutung der Analyse von T-Zellrezeptor-Genumlagerungen beim kutanen T-Zell-Lymphom

Assaf¹

2004

Akt Dermatol

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ZusammenfassungKutane T-Zell-Lymphome (CTCL) stellen eine Gruppe lymphoproliferativer Hauterkrankungen dar, die durch klonale Akkumulation von T-Lymphozyten-Populationen charakterisiert sind. Die Diagnose wird in der Regel aufgrund klinischer und histologischer charakteristischer Eigenschaften der CTCL gestellt. Schwierigkeiten bereitet die Diagnosestellung insbesondere in frühen Stadien, aber auch in seltenen Varianten der CTCL. In diesen Fällen ist der Nachweis klonaler T-Zell-Populationen durch identisch umgelagerte T-Zellrezeptor-Gene eine wertvolle Hilfe. So zeigen PCR-basierende Techniken zum Nachweis klonaler T-Zell-Populationen eine hohe Sensitivität von 70 ± 90 % in Hautbiopsien früher CTCL-Stadien. Nach Diagnosestellung ist der nächste wichtige Schritt die Ausbreitungsdiagnostik des CTCL, sinkt doch die Überlebensrate drastisch bei Auftreten extrakutaner Manifestationen wie z.B. bei Infiltration der Lymphknoten oder viszeraler Organe. Ein Stadienwechsel, d.h. die Progression der Erkrankung, wird in der Regel klinisch erst spät entdeckt. Insbesondere der Lymphknotenbefall, der erfahrungsgemäû als erste extrakutane Manifestation des CTCL gilt, stellt sowohl den Kliniker als auch den Histologen vor diagnostische Probleme. Hier konnten wir in einer aktuellen Studien zeigen, dass in ca. 50 % der dermatopathischen Lymphknoten identische klonale T-ZellPopulationen wie in den CTCL-Läsionen der Haut nachweisbar sind. Des Weiteren zeigte sich, dass diese Patienten eine reduzierte Überlebensrate haben, ähnlich denen, die eine histologisch manifeste Lymphknoteninfiltration durch das CTCL haben. Diese Daten belegen, dass T-Zellrezeptor-Umlagerungsanalysen bei Patienten mit CTCL sowohl eine diagnostische als auch prognostische Bedeutung haben und somit ein akkurates Staging ermöglichen. AbstractCutaneous T-cell lymphoma (CTCL) is a clonal lymphoproliferative malignancy primarily involving the skin. Routine diagnosis of CTCL is based on its characteristic clinical and histopathologic features. However, the broad clinical and histological spectrum of the disease, especially of its early stages and rare variants, often complicates the differentiation between malignant CTCL and benign lymphoproliferative or reactive skin diseases. In these cases demonstration of a clonal T-cell population by detection of identically rearranged T-cell receptor genes (TCR) provides an adjunct. Using this approach clonality could be demonstrated in 70-90 % in CTCL biopsies of early stages by recent PCR-based studies, indicating a high sensitivity. After achieving the correct diagnosis the next important step is to stage the disease in CTCLpatients. Patients with CTCL usually have an indolent course with a 5-year survival of approximately 87%. However, in a significant number of patients the disease may progress and disseminated extracutaneous manifestations may develop involving the lymph nodes, blood and visceral organs. The prognosis for patients with widespread manifestation of CTCL beyond the skin is poor. Therefore, accurate evaluatio...

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The diagnostic value of morphometry on blood lymphocytes in erythrodermic actinic reticuloid

Cited by 7 publications

References 0 publications

The diagnosis of Sézary syndrome on peripheral blood by flow cytometry requires the use of multiple markers

The diagnosis of Sézary syndrome on peripheral blood by flow cytometry requires the use of multiple markers

Karyometric Comparison of Splenic and Gastric Marginal Zone Lymphomas

Diagnostischer Stellenwert und klinische Bedeutung der Analyse von T-Zellrezeptor-Genumlagerungen beim kutanen T-Zell-Lymphom

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