The developmental proteome of <i>Drosophila melanogaster</i>

Casas-Vila, Núria; Bluhm, Alina; Sayols, Sergi; Dinges, Nadja; Dejung, Mario; Altenhein, Tina; Kappei, Dennis; Altenhein, Benjamin; Roignant, Jean‐Yves; Butter, Falk

doi:10.1101/gr.213694.116

Cited by 147 publications

(207 citation statements)

References 59 publications

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“…This developmentally induced starvation precedes the extended pupal feeding arrest. The RNA profiles matched proteomic profiles performed on whole animals that confirmed the increased NimB5 expression at the onset of pupariation and higher levels as animals reach the end of the pupal stage and emerge [46].…”

Section: Interestingly Published Modencode Developmental and Tissuesupporting

confidence: 55%

Metabolic adjustment ofDrosophilahemocyte number and sessility by an adipokine

Ramond

Petrignani

Dudzic

et al. 2019

Preprint

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In animals, growth is regulated by the complex interplay between paracrine and endocrine signals. When food is scarce, tissues compete for nutrients, leading to critical resource allocation and prioritization. Little is known about how the immune system maturation is coordinated with the growth of other tissues. Here, we describe a signaling mechanism that regulates the number of hemocytes (blood cells) according to the nutritional state of the Drosophila larva. Specifically, we found that the adipokine NimB5 is produced in the fat body upon nutrient scarcity downstream of metabolic sensors. NimB5 is then secreted and bind to hemocytes to down-regulate their proliferation and adhesion. Blocking this signaling loop results in conditional lethality when larvae are raised on a poor diet, due to excessive hemocyte numbers and insufficient energy storage. Similar regulatory mechanisms shaping the immune system in response to nutrient availability are likely to be widespread in animals. Short title: The adipokine NimrodB5 regulates peripheral hematopoiesis in Drosophila Author summaryDrosophila larval hemocytes (blood cells) are found in two compartments: the lymph gland considered as a reservoir, and the peripheral compartment. Peripheral hemocytes form sessile patches attached to the internal surface of the larval body wall or are found freely circulating in the hemolymph. Little is known about the signals that regulate hemocytes proliferation and localization in the peripheral compartment. In this study, we have identified a new gene, NimrodB5, coding for the NimB5 protein, which is secreted by the fat body and binds to hemocytes. NimB5 inhibits hemocyte proliferation while promoting sessility, leading to an increased number of circulating hemocytes and adhesion defects in NimB5 mutant. We show that nimrodB5 expression by the fat body is controlled by metabolic cues to adjust hemocyte number to the physiological state of the larvae.Interestingly, deregulation of NimB5 causes lethality when larvae are raised on a poor diet due to a defect in regulating hemocytes proliferation. In conclusion, we have identified a new adipokine that optimizes hemocytes number to the physiological state of larvae. Our study also reveals a major role of the fat body in peripheral hematopoiesis regulation and outline how it can be costly to maintain a basal immune defense.

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Section: Interestingly Published Modencode Developmental and Tissuesupporting

confidence: 55%

Metabolic adjustment ofDrosophilahemocyte number and sessility by an adipokine

Ramond

Petrignani

Dudzic

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…PCC is a well‐established measure of correlation and ranges from + 1 (perfect correlation) to −1 (perfect but negative correlation), with 0 denoting the absence of a relationship (Adler and Parmryd, ). The PCC value ( r = 0.228) obtained in the present study revealed that the overall correlation between secretomic and transcriptomic data was weak, consistent with the familiar study on Drosophila melanogaster (Casas‐Vila et al ., ). The weak correlation between secretomic and transcriptomic data may be mainly attributed to the influence and regulation of transcription and translation by various factors that drastically affected the expression level of mRNAs and the accumulation of secreted proteins, underscoring the importance of proteomics to study invasion progression.…”

Section: Discussionmentioning

confidence: 97%

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot

Yong

Fang

et al. 2019

Microbial Biotechnology

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Summary Target spot is a newly emerging citrus disease caused by Pseudofabraea citricarpa. Outbreaks of this disease result in massive economic losses to citrus production. Here, an integrated study involving comparative transcriptomic and secretomic analyses was conducted to determine the critical pathogenicity factors of P. citricarpa involved in the induction of citrus target spot. A total of 701 transcripts and their cognate proteins were quantified and integrated. Among these transcripts and proteins, 99 exhibited the same expression patterns. Our quantitative integrated multi‐omic data highlight several potentially pivotal pathogenicity factors, including 16 unigenes that were annotated as plant cell‐wall‐degrading enzymes, 13 unigenes homologous to virulence factors from various fungi, and one unigene described as a small cysteine‐rich secreted protein, were screened and analysed. The screening of differentially expressed genes that encode secondary metabolism core enzymes implicated terpene metabolism in the pathogenicity of P. citricarpa. Overall, results indicated that plant cell wall degradation, plant–pathogen protein/polyribonucleotide interaction, and terpene biosynthesis have critical roles in the pathogenicity of P. citricarpa. This work demonstrated that integrated omic approaches enable the identification of pathogenicity/virulence factors and provide insights into the mechanisms underlying the pathogenicity of fungi. These insights would aid the development of effective disease management strategies.

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“…These include: the chitin-based cuticle that forms the animal's exoskeleton and lines the lumens of the foregut and hindgut [63][64][65]; noncuticular, chitin-based ECMs that line the lumens of the trachea, salivary glands, and midgut [64,66]; the eggshell that protects the developing embryo [67,68]; and the salivary glue that is produced by the larva to affix the pupa to a surface [69]. Defining the list of proteins that comprise these Drosophila-specific ECMs will provide a reference dataset for the arthropod clade and aid with the annotation of large proteomic datasets, including the developmental proteome of Drosophila [70]. Moreover, because insects can be both disease vectors and agricultural pests, these data could provide an important source of information to combat these threats to human welfare.…”

Section: Introductionmentioning

confidence: 99%

In-silicodefinition of theDrosophila melanogastermatrisome

Davis

Horne‐Badovinac

Naba

2019

Preprint

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The extracellular matrix (ECM) is an assembly of hundreds of proteins that structurally supports the cells it surrounds and biochemically regulates their functions. Drosophila has emerged as a powerful model organism to study fundamental mechanisms underlying ECM protein secretion, ECM assembly, and ECM roles in pathophysiological processes. However, as of today, we do not possess a well-defined list of the components forming the ECM of this organism. We previously reported the development of computational pipelines to define the matrisome -the ensemble of genes encoding ECM and ECM-associated proteins -of humans, mice, zebrafish and C. elegans.Using a similar approach, we report here that the Drosophila matrisome is composed of 641 genes.We further classify these genes into different structural and functional categories, including an expanded way to classify genes encoding proteins forming apical ECMs. We illustrate how having a comprehensive list of Drosophila matrisome proteins can be used to annotate large proteomic datasets and identify unsuspected roles for the ECM in pathophysiological processes. Last, to aid the dissemination and usage of the proposed definition and categorization of the Drosophila matrisome by the scientific community, our list has been made available through three public portals: The Matrisome Project, FlyBase, and GLAD.

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The developmental proteome of Drosophila melanogaster

Cited by 147 publications

References 59 publications

Metabolic adjustment ofDrosophilahemocyte number and sessility by an adipokine

Metabolic adjustment ofDrosophilahemocyte number and sessility by an adipokine

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot

In-silicodefinition of theDrosophila melanogastermatrisome

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