The developmental effect of a sex‐limited gene in Drosophila melanogaster

Fung, Sui‐Tong Chan; Gowen, John W.

doi:10.1002/jez.1401340306

Cited by 22 publications

(9 citation statements)

References 17 publications

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“…However, the positive chemotaxis observed with Oregon R females tested with NaCl was not expected from previous observations. One finding that may be relevant to this point is that dsxD intersexes, whose ovipositors are rudimentary and heavily sclerotized (18), avoid NaCl. In Phormia there are chemoreceptors on the ovipositor that respond electrically to NaCl (19,20); if analogous receptors mediate the behavior of normal Drosophila females to NaCl, then dsxD flies would be expected to behave more like males, because their ovipositors do not appear to bear functional chemoreceptors.…”

Section: Resultsmentioning

confidence: 98%

Isolation and analysis of chemosensory behavior mutants in Drosophila melanogaster.

Tompkins¹,

Cardosa²,

White³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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flies, 2-4 days posteclosion) were introduced into the first vial in the row that contained the chemical stimulus and were allowed to distribute themselves between the two opposing stimulus-containing and non-stimulus-containing vials. At the end of the trial (6 hr with NaCl, 8 hr with quinine sulfate), the apparatus was manipulated as described by Benzer (5) to bring each vial containing flies opposite a fresh vial, thus beginning a new trial. Each experiment consisted of five such trials, after which flies were distributed in six fractions according to their tendency to choose a stimulus-containing vial. If the vial positions are consecutively numbered, with the vial in which flies were initially placed being designated zero, the fraction in which an individual is found corresponds to the number of times that the fly has appeared in the non-stimulus-containing vial at the end of a trial. Assuming that each individual acts independently and with a constant partition coefficient P, the distribution of flies recovered is expressed according to the binomial expansion (5, 6) as:where N is the number of flies in the total population, NM the number in fraction r, n the number of trials, and P the probability that a fly chooses a non-stimulus-containing vial. In our experiments, with n = 5, N and N, were determined by counting the flies at the end of the experiment, and, thus, P could be estimated by fitting the data to theoretical curves of known P values. Flies that were unresponsive to the stimulus (i.e., atactic or "taste-blind") should be distributed with P = 0.5; a P value less than 0.5 indicates attraction to the stimulus (a mistactic response in comparison to that of wild-type males) and, conversely, a P value greater than 0.5 indicates aversion.Assay of Proboscis Extension. To test the responses of individual flies to sucrose, adults (2-4 days posteclosion) were starved for 24 hr in a humid atmosphere and then lightly anesthetized with carbon dioxide and restrained by embedding Abbreviation: CCD, chemosensory countercurrent distribution.

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Section: Resultsmentioning

confidence: 98%

Isolation and analysis of chemosensory behavior mutants in Drosophila melanogaster.

Tompkins¹,

Cardosa²,

White³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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“…Male (XY) and female (XX) dsx null flies are dsx d+r3 /dsx m+r15 ( Duncan and Kaufman 1975 ; Baker et al 1991 ). The dsx D pseudo males (XX) are w,P{w +mc , Ubi-GFP}/+;dsx D , Sb 1 , e 1 /dsx m+r15 (for dsx D see Fung and Gowen 1957 ). The data for all experiments were generated at the same time, under the same environmental conditions.…”

Section: Methodsmentioning

confidence: 99%

Sex Differences in Drosophila Somatic Gene Expression: Variation and Regulation by doublesex

Arbeitman

New

Fear

et al. 2016

G3 Genes|Genomes|Genetics

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Sex differences in gene expression have been widely studied in Drosophila melanogaster. Sex differences vary across strains, but many molecular studies focus on only a single strain, or on genes that show sexually dimorphic expression in many strains. How extensive variability is and whether this variability occurs among genes regulated by sex determination hierarchy terminal transcription factors is unknown. To address these questions, we examine differences in sexually dimorphic gene expression between two strains in Drosophila adult head tissues. We also examine gene expression in doublesex (dsx) mutant strains to determine which sex-differentially expressed genes are regulated by DSX, and the mode by which DSX regulates expression. We find substantial variation in sex-differential expression. The sets of genes with sexually dimorphic expression in each strain show little overlap. The prevalence of different DSX regulatory modes also varies between the two strains. Neither the patterns of DSX DNA occupancy, nor mode of DSX regulation explain why some genes show consistent sex-differential expression across strains. We find that the genes identified as regulated by DSX in this study are enriched with known sites of DSX DNA occupancy. Finally, we find that sex-differentially expressed genes and genes regulated by DSX are highly enriched on the fourth chromosome. These results provide insights into a more complete pool of potential DSX targets, as well as revealing the molecular flexibility of DSX regulation.

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“…It arises from segment 9 primordium in the female genital disc. Synonyms: appendicular gland (Cumming and Wood, 2017), colleterial gland (Cumming and Wood, 2017), parovarium (Okada, 1956;Fung and Gowen, 1957;Bock and Wheeler, 1972;Epper, 1983;Chen and Baker, 1997;Cumming and Wood, 2017) Female accessory gland duct FlyBase ID: FBbt:00004915. Definition: A duct connecting the female accessory gland to the uterus, opening just caudal to the spermathecal ducts.…”

Section: Female Accessory Glandmentioning

confidence: 99%

“…FlyBase synonyms: spermathecum [sic] (FBbt:00004921) Definition: A mushroom-shaped organ consisting of a capsule (end apparatus) connected to the uterus by a slender duct. Sperm is stored in this organ after mating (Sturtevant, 1921;Salles, 1947;Okada, 1956;Fung and Gowen, 1957;Throckmorton, 1962;Hildreth, 1965;Bock and Wheeler, 1972;Epper, 1983;Chen and Baker, 1997;Heifetz and Rivilin, 2010). The capsule is an inverted, double-walled bowl, i.e., outer capsule and basal introvert consisting of schlerotized intima (Pitnick et al, 1999;Mayhew and Merritt, 2013).…”

Section: Spermathecamentioning

confidence: 99%

A Standardized Nomenclature and Atlas of the Female Terminalia of <em>Drosophila melanogaster</em>

McQueen¹,

Afkhami²,

Atallah³

et al. 2022

Preprint

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The model organism Drosophila melanogaster has become a focal system for investigations of rapidly evolving genital morphology as well as the development and functions of insect reproductive structures. To follow up on a previous paper outlining unifying terminology for the structures of the male terminalia in this species, we offer here a detailed description of the female terminalia of D. melanogaster. Informative diagrams and micrographs are presented to provide a comprehensive overview of the external and internal reproductive structures of females. We propose a collection of terms and definitions to standardize the terminology associated with the female terminalia in D. melanogaster and we provide a correspondence table with the terms previously used. Unifying terminology for both males and females in this species will help to facilitate communication between various disciplines, as well as aid in synthesizing research across publications within a discipline that has historically focused principally on male features. Our efforts to refine and standardize the terminology should expand the utility of this important model system for addressing questions related to the development and evolution of animal genitalia, and morphology in general.

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The developmental effect of a sex‐limited gene in Drosophila melanogaster

Cited by 22 publications

References 17 publications

Isolation and analysis of chemosensory behavior mutants in Drosophila melanogaster.

Isolation and analysis of chemosensory behavior mutants in Drosophila melanogaster.

Sex Differences in Drosophila Somatic Gene Expression: Variation and Regulation by doublesex

A Standardized Nomenclature and Atlas of the Female Terminalia of <em>Drosophila melanogaster</em>

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