The Determination of Berberine by Ion-pair Extraction-Titration, with Tetrabromophenolphthalein Ethyl Ester as the Indicator

Tsubouchi, Masahiro

doi:10.1246/bcsj.52.2581

Cited by 7 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the literature, the berberine cation has been associated with various organic and inorganic anions, ,, and it has been observed that many of these water-soluble salts easily gave nice single crystals, the structure of which has been reported. − In the present paper, our idea was to associate the berberine cation with a carboxylate anion provided with a long alkyl chain, specifically palmitate, in order to obtain a hydrophobic salt (Figure ) and with the hope that this salt would crystallize in water. The following work describes the preparation and characterization of berberine palmitate, as well as its spectroscopic behavior in solution and in the solid state.…”

Section: Introductionmentioning

confidence: 95%

Spontaneous Formation of Fluorescent Nanofibers and Reticulated Solid from Berberine Palmitate: A New Example of Aggregation-Induced Emission Enhancement in Organic Ion Pairs

et al. 2011

View full text Add to dashboard Cite

The salt formed between the large aromatic berberine cation and the long-chain palmitate anion was synthesized and used to prepare aqueous suspensions of particles owing to a solvent-exchange method. Under these conditions, elongated particles were readily obtained. They were studied by transmission microscopy with polarized light, as well as by fluorescence and electron microscopy. They were shown to be probably crystallized nanofibers, which were stable in suspension. Unexpectedly, upon filtration and drying, these fibers evolved to give a reticulated solid. The fluorescence properties of the compound were analyzed in solution, in aqueous suspension and in the powder crystalline state. Interestingly, berberine palmitate is virtually not fluorescent in aqueous solution because of the quenching effect of water, but transition to the solid state was accompanied by a strong increase in fluorescence intensity. This phenomenon was explained by the original molecular arrangement in the solid state. Actually, in the crystal, the anions form a distinct layer, which limits parallel-stacking of the fluorescent cations. Moreover, the berberine cations are protected from the access of water molecules, and so no quenching effect can take place. This example confirms that the newly introduced concept of ion-pair aggregation-induced fluorescence enhancement can be extended to a variety of structures. It also shows the interest of ion pairs for preparing fluorescent nanofibers and reticulated solids using a solvent-exchange method that is particularly easy to implement.

show abstract

Section: Introductionmentioning

confidence: 95%

Spontaneous Formation of Fluorescent Nanofibers and Reticulated Solid from Berberine Palmitate: A New Example of Aggregation-Induced Emission Enhancement in Organic Ion Pairs

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The two-phase ion-association titration was applied to the determination of some basic pharmaceutical compounds using indicators for visible end-point detection. Earlier, the end-point detection in this type of titrations was based on the movement of the indicator from one phase to other and it was difficult to detect the end point because the color of the indicator in the aqueous phase or organic phase will be reflected in the other phase [39]. The use of hydrophobic indicator is an alternative approach, which remains in the organic phase throughout the titration and gives a very sharp color change [40].…”

Section: Resultsmentioning

confidence: 99%

Application of Ion-Association Titration for the Assay of Cyproheptadine Hydrochloride in Pharmaceuticals

Raghu

Basavaiah

2012

ISRN Analytical Chemistry

View full text Add to dashboard Cite

Two simple and rapid titrimetric methods are described for the determination of cyproheptadine hydrochloride (CPH) in pharmaceuticals. The proposed methods are based on the solvent extraction-titration of CPH with two ion association reagents, sodium lauryl sulphate (SLS), (method A), and tetraphenylborate (TPB), (method B). In method A, SLS was used as titrant and the titration was carried out in the presence of dilute sulphuric acid and chloroform using dimethyl yellow as indicator, whereas, in method B, the titrant was TPB and the titration was done in Walpole buffer of pH 4.5 and 1, 2-dichloroethane using tetrabromophenolphthalein ethyl ester as indicator. The proposed procedures give sharp end points as the color of the organic phase changes from yellow to pink in method A, and from red-violet to yellow in method B. The methods are applicable over the ranges of 1.0-20.0 and 2.0-9.0 mg of CPH for method A and method B, respectively. The accuracy and precision of the methods are good. The methods were applied successfully to the determination of CPH in tablets, and the results were in agreement with the label claim and those of the reference method.

show abstract

“…The method provides a simple operation to quantitativly analyze berberine. This method, for example, has better sensitivity for berberine than the assay of berberine in bulk aqueous solution with optical fiber sensor [16,17,18], titrimetry [5,6], and spectorfluorimetric [13] methods. Although, spectrofluorometry [14,15] involving liquid-liquid extraction is sensitive, it requires large amounts of organic solvent.…”

Section: Discussionmentioning

confidence: 99%

“…There are many methods for its assay in plant extracts and in pharmaceutical preparations such as titrimetry [5,6], spectrophotometry [7,8,9,10], TLC [11] and HPLC [12]. These methods, however, have suffered from drawbacks such as being tedious, time-consuming and of low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of berberine by measuring the enhanced total internal reflected fluorescence at water/tetrachloromethane interface in the presence of sodium dodecyl benzene sulfonate

Feng

Huang

2003

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

A highly sensitive method for determination of berberine is proposed based on the measurements of total internal reflected fluorescence (TIRF) at water/ tetrachloromethane (H(2)O/CCl(4)) interface. In the pH range of 2.6-5.7, the co-adsorption of the berberine with the anionic surfactants such as sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDS), and sodium lauryl sulfate (SLS) occurs at the H(2)O/CCl(4 )interface, resulting in greatly enhanced TIRF signal characterized by the emission at 526 nm when excited with a 351 nm light beam. The enhanced TIRF intensity is in proportion to the berberine concentration in the range 0.2-10.0x10(-7) mol L(-1). The limit of detection is 1.7x10(-9) mol L(-1) (3sigma). It was found that ions such as Ca(II), Cu(II), Fe(III), Cd(II), Mg(II), Zn(II), Pb(II), and Al(III) can be allowed larger than 1.0x10(-4) mol L(-1). Meanwhile, the organic compounds such as vitamin B, saccharine, and amino acid do not display any effect for the present TIRF method even if they are larger than 1.0x10(-2) mol L(-1)in high concentration levels (larger than 1.0x10(-5) mol L(-1)). The results of determination for synthetic samples were agreement with the desired values, and the ones for tablets were identical with those obtained according to the method of Chinese Pharmacopoeia.

show abstract

The Determination of Berberine by Ion-pair Extraction-Titration, with Tetrabromophenolphthalein Ethyl Ester as the Indicator

Cited by 7 publications

References 10 publications

Spontaneous Formation of Fluorescent Nanofibers and Reticulated Solid from Berberine Palmitate: A New Example of Aggregation-Induced Emission Enhancement in Organic Ion Pairs

Spontaneous Formation of Fluorescent Nanofibers and Reticulated Solid from Berberine Palmitate: A New Example of Aggregation-Induced Emission Enhancement in Organic Ion Pairs

Application of Ion-Association Titration for the Assay of Cyproheptadine Hydrochloride in Pharmaceuticals

Determination of berberine by measuring the enhanced total internal reflected fluorescence at water/tetrachloromethane interface in the presence of sodium dodecyl benzene sulfonate

Contact Info

Product

Resources

About