The Detection of t(14;18) in Archival Lymph Nodes

Barrans, Sharon; Evans, Paul; O’Connor, Sheila J.M.; Owen, Roger G.; Morgan, Gareth J.; Jack, Andrew

doi:10.1016/s1525-1578(10)60469-2

Cited by 40 publications

(5 citation statements)

References 52 publications

(49 reference statements)

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“…This illustrates that FISH is a useful test in the diagnosis of follicular lymphomas, consistent with literature reports, [21][22][23][24] and will detect cases that even a comprehensive PCR approach will miss. Furthermore, this finding also suggests the presence of other novel, undetected breakpoints.…”

Section: Discussionsupporting

confidence: 85%

“…Although BCL6 is a relatively rare translocation in low-grade follicular lymphomas, it has been noted as a frequent gene alteration in grade 3 follicular lymphomas. 22 Four cases of the six found to contain BCL6 translocations in this study consisted of grade 3 follicular lymphomas ( Table 3). The remaining eight negative cases consisted of mostly grade 1 and 2 diseases (five and one case, respectively).…”

Section: Discussionmentioning

confidence: 79%

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″Minor″ BCL2 Breakpoints in Follicular Lymphoma

Weinberg

Mariappan

et al. 2007

The Journal of Molecular Diagnostics

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Follicular lymphomas are frequently associated with the t(14;18)(q32;q21). This translocation can be detected by karyotype, polymerase chain reaction (PCR), and fluorescence in situ hybridization (FISH). In addition to the breakpoints currently used for diagnosis located in the major breakpoint region (MBR) and the minor cluster region (mcr), recent studies have reported the existence of other breakpoints (3 BCL2, 5mcr, and icr). In this study, we examined the frequency of all five breakpoints in 236 cases of follicular lymphomas by real-time PCR analysis. The distribution of breakpoint sites consisted of MBR in 118 cases (50%), mcr in 11 (5%), icr in 32 (13%), 3 BCL2 in 13 (6%), and 5 mcr in three cases (1%). These findings illustrate significantly higher frequency of the icr breakpoint as compared with the more frequently studied mcr. Correlation of breakpoints with histology showed that MBR breakpoints occur more frequently in grade 2 lymphomas (P ‫؍‬ 0.042). A majority of the PCR-negative cases (

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 79%

″Minor″ BCL2 Breakpoints in Follicular Lymphoma

Weinberg

Mariappan

et al. 2007

The Journal of Molecular Diagnostics

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“…IgH/Bcl-2 rearrangement was studied using FISH [4] for the t(14;18) translocation in formalin-fixed, paraffin-embedded samples on all FL and reactive cases using the Vysis LSI IgH Spectrum Green/LSI BCL2 Spectrum Orange probe set (Vysis, No. 32-191018), which includes the probe and the hybridization buffer.…”

Section: Methodsmentioning

confidence: 99%

Diagnostic and Prognostic Utility of t(14;18) in Follicular Lymphoma

et al. 2007

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Background: Follicular lymphoma (FL) is one of the most common non-Hodgkin’s lymphomas of B cells, being closely associated with a t(14;18) translocation. Detection of t(14;18), which is present in 70–95% of FL, might aid in FL diagnosis. Objective: To compare the efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples of FL patients at different stages. Combined with other immunophenotypic biological determinants, detection of t(14;18) might help to determine patients at increased risk according to the FL International Prognostic Index (FLIPI) and therefore facilitate appropriate treatment. Design and Methods: This study was mainly based on a retrospective examination of formalin-fixed, paraffin-embedded lymph nodes. We selected fixed tissue samples of 21 FL patients treated at the National Cancer Institute Center in the period from 2000 to 2001. Results: FISH techniques could detect 14 of 18 FL cases with a sensitivity of 77.8%, while the PCR technique could detect only 11 of 18 FL cases with a sensitivity of 61.1%, resulting in a statistically significant difference between both techniques (p = 0.004). According to the FLIPI index, 9 of the 18 FL patients were categorized into the high-risk group (50%), 5 in the intermediated-risk group (27.8%) and 4 in the low-risk group (22.2%). Conclusion: The sensitivity of FISH is superior to that of PCR in the detection of the t(14;18) translocation in paraffin-embedded tissue samples. There is a statistically significant correlation between both CD10 and FISH with FLIPI.

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“…Other recurrent abnormalities observed are partial or complete gain of chromosome 3, specifically 3q; loss of chromosome 6; and gain of chromosome 18 and t(14;15) (q32.2;q11.2). Among these abnormalities, the only gain of chromosome 3 is associated with an adverse prognosis [83][84][85][86][87][88].…”

Section: Diffuse Large B-cell Lymphoma (Dlbcl)mentioning

confidence: 99%

Chromosome Abnormalities in Hematological Malignancies and Its Clinical Significance

Subramanian¹

2022

Down Syndrome and Other Chromosome Abnormalities

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The latest version of the World Health Organization guidelines focuses mainly on the genetic and cytogenetic features of hematologic neoplasms as predictors of diagnostic, treatment decision, prognostic outcome, and for treatment monitoring in hematological malignancies. There are different techniques to identify these abnormalities. Live cells are needed for chromosome preparation. The Hematological malignancies include myeloid and lymphoid neoplasms. The myeloid neoplasms include Myelodysplastic syndromes, myeloproliferative neoplasms, and acute myeloid leukemias. The Lymphoid neoplasms include acute and chronic lymphocytic leukemias, plasma cell neoplasms, myeloma, hodgkin, and non-hodgkin lymphomas. The first chromosomal abnormality discovered in connection with cancer is the Philadelphia chromosome, which is an abnormal chromosome 22, formed due to the translocation between chromosomes 9 and 22. The presence of this abnormal chromosome confirms the diagnosis of “CML”. After that, hundreds of chromosomal abnormalities have been identified in hematological malignancies in different parts of the world. In AML, specific abnormalities were identified as having a good prognosis, intermediate prognosis, and poor prognosis. In other hematological malignancies also there some specific chromosome abnormalities are associated with prognostication. Now a day’s clinicians depend mainly on genetic abnormalities for the proper treatment management of hematological malignancies, so the study of chromosomal abnormalities is essential.

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The Detection of t(14;18) in Archival Lymph Nodes

Cited by 40 publications

References 52 publications

″Minor″ BCL2 Breakpoints in Follicular Lymphoma

″Minor″ BCL2 Breakpoints in Follicular Lymphoma

Diagnostic and Prognostic Utility of t(14;18) in Follicular Lymphoma

Chromosome Abnormalities in Hematological Malignancies and Its Clinical Significance

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