The detection of<i>Trypanosoma cruzi</i>by nested-PCR in elderly patients: relationship to the clinical and epidemiological profile

Pereira, Marcy Lancia; Batista, Angélica Martins; Aguiar, Camila; Marcon, Gláucia Elisete Barbosa; Martins, Luíz Cláudio; Guariento, Maria Elena; Costa, Sandra Cecı́lia Botelho; Almeida, Eros Antônio de

doi:10.1080/20477724.2016.1232850

Cited by 7 publications

(11 citation statements)

References 38 publications

(43 reference statements)

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“…The number of elderly travellers differed between European countries and ranged between 83.7% in Denmark and only 11.0% in Bulgaria [12]. In the study performed by Gautret et al [11], 49.1% of tourists aged 60 years and over were from Europe, 36.4% from North America and 6.7% from Asia. Destinations chosen by senior travellers are also important; travel to developing countries has become increasingly popular.…”

Section: Epidemiologymentioning

confidence: 99%

“…What is more, the clinical interaction between CD and other age-related illnesses may increase mortality and morbidity and may reduce the quality of life. Indeed, morbidity rates among the elderly are higher in comparison with other age groups [47][48][49]. Pereira et al [49] highlighted that the elderly patients with CD need special attention from healthcare providers and that further studies in this group of patients are necessary.…”

Section: Most Common Travel-associated Diseases Arthropod-borne Diseasesmentioning

confidence: 99%

“…Indeed, morbidity rates among the elderly are higher in comparison with other age groups [47][48][49]. Pereira et al [49] highlighted that the elderly patients with CD need special attention from healthcare providers and that further studies in this group of patients are necessary. Leishmaniasis is a disease caused by an infection with Leishmania parasites and is spread by the bite of phlebotomine sand flies.…”

Section: Most Common Travel-associated Diseases Arthropod-borne Diseasesmentioning

confidence: 99%

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The older traveller

Woś

Korzeniewski²

2018

International Maritime Health

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All over the world there has been a sudden increase in the number of international travels, mostly for touristic purposes. According to the World Tourism Organisation, the number of international journeys exceeded 1.323 billion in 2017 and it continues to grow. Of the growing number of travellers more and more are the elderly (> 65 years), this fact can be attributed to longer life expectancy and a better quality of life, especially in the developed countries. The article lists the main destinations chosen by senior tourists and their reasons for travel, it also discusses physiological changes in organs and systems affecting the elderly, which are the result of travelling across time zones and to areas with different environment. The article looks at various groups of patients affected by chronic diseases and examines health-related consequences of travel, including the most common complications. The general health prevention measures, with emphasis on vaccinations and antimalarial chemoprophylaxis, have also been discussed.

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Section: Epidemiologymentioning

confidence: 99%

Section: Most Common Travel-associated Diseases Arthropod-borne Diseasesmentioning

confidence: 99%

Section: Most Common Travel-associated Diseases Arthropod-borne Diseasesmentioning

confidence: 99%

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The older traveller

Woś

Korzeniewski²

2018

International Maritime Health

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“…In Brazil, there are approximately 5,700 kidney transplants each year [13], with a progressive increase for this intervention in elderly patients because of renal disease [14]. This scenario favors reactivation of the disease since most patients with chronic Chagas disease are elderly and present a variety of comorbidities [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

Ferreira

Almeida

Marcon

et al. 2021

J Infect Dev Ctries

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Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data on the parasitic load in long-term organ recipients. In this study, we examined the parasitic load in long-term kidney transplant patients and assessed the possibility of late Chagas disease reactivation. Methodology: Blood cultures and real-time PCR were used to assess the parasitic load in four immunosuppressed patients who underwent kidney transplants (between 1996 and 2014) and were also treated for parasites. Results: There were no positive blood culture or real-time PCR results in Chagas disease patients who received kidney transplants. The real-time PCR presented detection limit of 0.1 parasite equivalent/mL. The time interval between the transplant and sample collection varied from one to 19 years. Conclusions: No parasites were detected in the evaluated patients. The use of benznidazole and immunosuppressive therapy may have contributed to control the T. cruzi infection. In transplanted patients with Chagas disease, the use of methods such real-time PCR and blood culture can monitor the parasitic load and prevent disease reactivation.

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“…Most of the strategies for the evaluation of T. cruzi transmission, epidemiology and control are dependent on methods of detection in tissues. Samples from humans [3] [4], animal models [5] [6] [7] and Triatominae insects [8] [9] have been exhaustively analyzed for elucidation of mechanisms and control of Chagas disease.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of <em>Trypanosoma cruzi</em> from formalin fixed placentas and fetuses of Wistar rats

Costa

Pereira

Júnior

et al. 2019

Matters

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Chagas disease is a tropical disease, endemic of the Americas and caused by the protozoan Trypanosoma cruzi. The classic transmission route of T. cruzi is based on the contact with feces from Triatominae insects containing trypomastigotes forms of the parasite. T. cruzi is also spread by oral and congenital forms, besides blood donation and organ transplant transmission. The parasite has a tropism to several organs and tissues, including heart, brain, digestive tract, muscles, placenta, and fetuses. The determination of parasite burden in organs is an important parameter for scientific assays related to parasite control and epidemiology. Among the procedures to detect and quantify T. cruzi, PCR demonstrates high sensitivity and specificity. In this study, we developed a simple and fast method to detect T. cruzi from fixed placentas and fetuses of Wistar rats. T. cruzi was detected only in tissues from infected animals, with different parasite burdens. Once the histology usually demonstrates some limitations such as low sensitivity and specificity to detect T. cruzi in fetuses, our methodology will be useful for fixed fetal tissues from organ banks. The detection of T. cruzi from gestational tissues will contribute to the elucidation of mechanisms related to congenital Chagas disease.

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The detection ofTrypanosoma cruziby nested-PCR in elderly patients: relationship to the clinical and epidemiological profile

Cited by 7 publications

References 38 publications

The older traveller

The older traveller

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

Molecular detection of <em>Trypanosoma cruzi</em> from formalin fixed placentas and fetuses of Wistar rats

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