The detection of horse and donkey using real-time PCR

Chisholm, James; Conyers, C.; Booth, Christine S.; Lawley, Wendy J.; Hird, Heather

doi:10.1016/j.meatsci.2005.03.009

Cited by 55 publications

(37 citation statements)

References 13 publications

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“…It is successfully used for identification of various species of meat (Frezza et al 2008 andMane et al, 2009). Also, Chisholm et al, (2005) developed real-time PCR assays specific for horse and donkey, applicable for detection of low levels of horse or donkey meat in commercial products.…”

Section: Discussionmentioning

confidence: 99%

Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Mousa¹,

Nashwa²,

Helmy³

et al. 2017

AJVS

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Section: Discussionmentioning

confidence: 99%

Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Mousa¹,

Nashwa²,

Helmy³

et al. 2017

AJVS

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“…It brings away the demand for immunological and electrophoretic methods, and minimizes the risk of contamination during the testing 63 . Real Time PCR has a sensitivity in detection of meat species by 0.1% whereas ELISA can do it less sensitive by 2% 21,32,35,[64][65][66][67][68][69][70][71] . DNA Microarray and Real Time PCR methods differentiate from each other in simultaneously detection of animal species in one reaction.…”

Section: Discussionmentioning

confidence: 99%

Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods

Özpınar¹,

Tezmen²,

Gökçe³

et al. 2013

Kafkas Univ Vet Fak Derg

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Determination of animal species in the meat and meat products is one of the interest of food science, it is also important for consumer rights and food safety. Increasing world population has also remarkably impacted the demand for meat and meat products. Based on this fact, issues related to safety and quality in the meat products have brought up in that manner as well. Through DNA based molecular methods are improved in food analysis, it is preferred increasingly in the control of food safety. In this study, a total of 73 samples of the meat and meat products sold in stores, meat selling markets and public bazaars located in different districts of İstanbul province were analyzed for the detection of animal species notified on the label by using Chipron LCD Array Analysis System. The results showed that 39 samples (53.4%) were labelled incorrectly. Randomly selected eleven samples were corrected by Iontek Fluorion Meat Species Identification Kit and FDS Detection System (Real Time PCR). Hence, it was found that the results obtained by DNA Microarray and Real Time PCR methods were identical (100%) with each other, and both methods should extensively be promoted for the detection of animal species in the meat and meat products.

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“…PCR was performed according to a previously published protocol (Chisholm, Conyers, Booth, Lawley, & Hird, 2005) in 50 μl final volume solutions, using the GoTaq Hot Start Master Mix (Promega Gmbh, Mannheim, 68199, Germany), 1 mM each of the primers Forward: GAC CTC CCA GCT CCA TCA AAC ATC TCA TCT TGA TGA AA and Reverse: CTC AGA TTC ACT CGA CGA GGG TAG TA amplifying a 439 bp gene, and 10 μl of eluted DNA. PCR products were separated in 2% agarose gel, stained with ethidium bromide (0.5 μg/ml) and documented under UV illumination.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Effectual Gold Nanoprobe Sensor for Screening Horse Adulteration in Meat Products

Houhoula¹,

Kouzilou²,

Tzogias³

et al. 2017

JFR

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A gold nanoparticle (AuNP) probe strategy for testing meat authenticity was developed, which relies on the colorimetric differentiation of a particular DNA sequence, due to the differential aggregation profiles exhibited by the AuNPs in the presence or absence of specific target hybridization. Gold nanoparticles were conjugated with thiolated oligonucleotides for specifically identifying a 69 bp fragment of the horse cytochrome b gene. In the presence of a complementary target preventing aggregation of the AuNPs when acid was added, the reaction mixtures retained the original pink colouration of the colloidal particles, whereas they turned purple in the opposite event. Fresh meatballs, prepared using pure bovine meat, were used as blanks, producing a purplish coloured solution with a peak at ≥570nm. Horse meat was used as positive control and the pink colour obtained after hybridization exhibited maximum absorption at 524 nm. Both the specificity and sensitivity of the tests performed were 100%. Visual observations and spectroscopic data indicated that the coloration produced by the AuNPs (positive-pink, negative-purple) was very stable, showing no change under normal laboratory conditions. The use of AuNPs for the colorimetric detection of DNA targets from undeclared species in meat products provides an inexpensive and easy-to-perform alternative to common molecular assays. The technology described here can be further developed to accommodate detection of many cases of adulteration and fraudulent practices.

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The detection of horse and donkey using real-time PCR

Cited by 55 publications

References 13 publications

Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods

Effectual Gold Nanoprobe Sensor for Screening Horse Adulteration in Meat Products

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