The Design and Simulation of a Planar Microarray Dot Electrode for a Dielectrophoretic Lab-on-Chip Device

Yafouz, Bashar; Kadri, Nahrizul Adib; Ibrahim, Fatimah

doi:10.1016/s1452-3981(23)16525-4

Cited by 13 publications

(1 citation statement)

References 23 publications

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“…Shrinkage in width is more dominant for thick structures since higher lateral surface area makes degassing easier, hence decreasing the width and certainly increasing the gap. From equation (1), the electrical field gradient depends on the gap between electrodes, which require a higher voltage to meet the essential DEP force [22]. Thus, the gap between SU-8 structures should be narrower than the desired gap between carbon electrodes.…”

Section: Optical Characterizationmentioning

confidence: 99%

Fabrication of a 3D carbon electrode for potential dielectrophoresis-based hepatic cell patterning application using carbon micro-electrical-mechanical system (CMEMS)

Yahya¹,

Ibrahim²,

Thiha³

et al. 2022

J. Micromech. Microeng.

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Cell patterning of hepatocyte cells is one of the techniques to construct liver tissue engineering. This work presents the development of a 3D carbon dielectrophoresis (carbonDEP) microfluidic chip for cell patterning using carbon micro-electrical-mechanical system (CMEMS) microfabrication approach. The new design of electrode named interdigitated radiating-strips electrode (IRSE) was fabricated to generate positive DEP (pDEP) force for cell patterning mimicking the biological hepatic lobule. The electrical characterization of the fabricated carbon electrode shows that the average electrode resistivity is 4.61 ± 1.19 x 10-4 Ω.m which is low enough to generate effective DEP force using ten of volts. Results also show the shrinkage of the SU-8 structures during pyrolysis which gives impact to the final dimension of the carbon electrode. The functioning of the DEP microfluidic chip was demonstrated through DEP polystyrene microbeads patterning as a model of hepatocyte cells. 3D carbon IRSE presents a 67% increase in trapping efficiency of pDEP microbeads as compared to the planar carbon IRSE and the microbeads were pattern along the electrical field induced to form a hepatic lobule mimicking pattern. These results suggest that the 3D carbonDEP microfluidic chip has a great potential to be used for 3D hepatic cells patterning for liver tissue engineering applications.

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Section: Optical Characterizationmentioning

confidence: 99%

Fabrication of a 3D carbon electrode for potential dielectrophoresis-based hepatic cell patterning application using carbon micro-electrical-mechanical system (CMEMS)

Yahya¹,

Ibrahim²,

Thiha³

et al. 2022

J. Micromech. Microeng.

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Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

et al. 2015

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Dielectrophoresis (DEP), the induced movement of dielectric particles placed in a nonuniform electric field, has been used as a potential technique for manipulation and separation of many biological samples without destructive consequences to the cell. Cells of the same genotype in different physiological and pathological states have unique morphological and structural features, therefore, it is possible to differentiate between them using their DEP responses. This paper reports the experimental discrimination of normal and dengue-infected human hepatic fetal epithelial cells (WRL-68 cells) based on their DEP crossover frequency, at which no resultant movement occurs in the cells in response to the DEP force. A microarray dot electrode was used to conduct the DEP experiments. The DEP forces applied to the cells were quantified by analyzing the light intensity shift within the electrode's dot region based on the Cumulative Modal Intensity Shift image analysis technique. The differences in dielectric properties between infected and uninfected cells were exploited by plotting a unique DEP spectrum for each set of cells. We observed that the crossover frequency decreased from 220 kHz for the normal WRL-68 cells to 140 kHz after infection with the dengue virus in a medium conductivity of 100 μS/cm. We conclude that the change in the DEP crossover frequency between dengue-infected cells and their healthy counterparts should allow direct characterization of these cell types by exploiting their electrophysiological properties.

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Microfluidic device embedding electrodes for dielectrophoretic manipulation of cells‐A review

et al. 2018

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Microfluidic device embedding electrodes realizes cell manipulation with the help of dielectrophoresis. Cell manipulation is an important technology for cell sorting and cell population purification. Till now, the theory of dielectrophoresis has been greatly developed. Microfluidic devices with various arrangements of electrodes have been reported from the beginning of the single non‐uniform electric field to the later multiple physical fields. This paper reviews the research status of microfluidic device embedding electrodes for cell manipulation based on dielectrophoresis. Firstly, the working principle of dielectrophoresis is explained. Next, cell manipulation approaches based on dielectrophoresis are introduced. Then, different types of electrode arrangements in the microfluidic device for cell manipulation are discussed, including planar, multilayered and microarray dot electrodes. Finally, the future development trend of the dielectrophoresis with the help of microfluidic devices is prospected. With the rapid development of microfluidic technology, in the near future, high precision, high throughput, high efficiency, multifunctional, portable, economical and practical microfluidic dielectrophoresis will be widely used in the fields of biology, medicine, agriculture and so on.

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The Design and Simulation of a Planar Microarray Dot Electrode for a Dielectrophoretic Lab-on-Chip Device

Cited by 13 publications

References 23 publications

Fabrication of a 3D carbon electrode for potential dielectrophoresis-based hepatic cell patterning application using carbon micro-electrical-mechanical system (CMEMS)

Fabrication of a 3D carbon electrode for potential dielectrophoresis-based hepatic cell patterning application using carbon micro-electrical-mechanical system (CMEMS)

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

Microfluidic device embedding electrodes for dielectrophoretic manipulation of cells‐A review

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