The cytoskeleton of retinal pigment epithelial cells

A Rapid Method for Isolation of Retinal Pigment Epithelial Cells from Rat Eyeballs

“…Using dispase, Chang et al [5] isolated RPE cells with only one enzyme digestion step. The RPE cells Chang et al [5] obtained were hexa gonal, formed junctional complexes similar to cells in vivo, and stained with antikeratin antibody or phalloidin along their borders, all characteristics seen in rat retinas in situ [12], We confirmed that the dispase method is effective for isolating RPE cells from rats 6-8 days old, but we also found that the method has some limitations. First, dispase was too mild to isolate RPE sheets from eyes of the older rats we used (9-15 days).…”

A Rapid Method for Isolation of Retinal Pigment Epithelial Cells from Rat Eyeballs

“…Using dispase, Chang et al [5] isolated RPE cells with only one enzyme digestion step. The RPE cells Chang et al [5] obtained were hexa gonal, formed junctional complexes similar to cells in vivo, and stained with antikeratin antibody or phalloidin along their borders, all characteristics seen in rat retinas in situ [12], We confirmed that the dispase method is effective for isolating RPE cells from rats 6-8 days old, but we also found that the method has some limitations. First, dispase was too mild to isolate RPE sheets from eyes of the older rats we used (9-15 days).…”

A new wound healing model of retinal pigment epithelial cells in sheet culture

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Ganglion cell loss in RCS rat retina: A result of compression of axons by contracting intraretinal vessels linked to the pigment epithelium

“…The contractile filaments myosin I and II have also been detected in the RPE cells of various mammals and fish (Breckler and Burnside, 1994). The presence of actin in RPE cells has been reported in numerous in vitro and in vivo studies (e.g., Burnside, 1976;Burnside and Laties, 1979;Chaitin and Hall, 1983;Haley et al, 1983;Owaribe, 1988;Fuchs et al, 1991) and has been shown to reorganise during migration (e.g., Campochiaro and Glaser, 1986) and regeneration (Korte et al, 1995). Lastly, some in vitro studies have shown that the interaction of RPE cells with matrix may cause contraction of the RPE cell sheets (Verstraeten et al, 1992;Smith-Thomas et al, 1996), and RPE cells have also been shown to mediate collagen contraction in vitro (Yamakawa et al, 1989;Raymond and Thompson, 1990;Grisanti and Guidry, 1995).…”

A Rapid Method for Isolation of Retinal Pigment Epithelial Cells from Rat Eyeballs

“…Using dispase, Chang et al [5] isolated RPE cells with only one enzyme digestion step. The RPE cells Chang et al [5] obtained were hexa gonal, formed junctional complexes similar to cells in vivo, and stained with antikeratin antibody or phalloidin along their borders, all characteristics seen in rat retinas in situ [12], We confirmed that the dispase method is effective for isolating RPE cells from rats 6-8 days old, but we also found that the method has some limitations. First, dispase was too mild to isolate RPE sheets from eyes of the older rats we used (9-15 days).…”

A new wound healing model of retinal pigment epithelial cells in sheet culture

“……”

Ganglion cell loss in RCS rat retina: A result of compression of axons by contracting intraretinal vessels linked to the pigment epithelium

“…The contractile filaments myosin I and II have also been detected in the RPE cells of various mammals and fish (Breckler and Burnside, 1994). The presence of actin in RPE cells has been reported in numerous in vitro and in vivo studies (e.g., Burnside, 1976;Burnside and Laties, 1979;Chaitin and Hall, 1983;Haley et al, 1983;Owaribe, 1988;Fuchs et al, 1991) and has been shown to reorganise during migration (e.g., Campochiaro and Glaser, 1986) and regeneration (Korte et al, 1995). Lastly, some in vitro studies have shown that the interaction of RPE cells with matrix may cause contraction of the RPE cell sheets (Verstraeten et al, 1992;Smith-Thomas et al, 1996), and RPE cells have also been shown to mediate collagen contraction in vitro (Yamakawa et al, 1989;Raymond and Thompson, 1990;Grisanti and Guidry, 1995).…”

A Rapid Method for Isolation of Retinal Pigment Epithelial Cells from Rat Eyeballs

“…Using dispase, Chang et al [5] isolated RPE cells with only one enzyme digestion step. The RPE cells Chang et al [5] obtained were hexa gonal, formed junctional complexes similar to cells in vivo, and stained with antikeratin antibody or phalloidin along their borders, all characteristics seen in rat retinas in situ [12], We confirmed that the dispase method is effective for isolating RPE cells from rats 6-8 days old, but we also found that the method has some limitations. First, dispase was too mild to isolate RPE sheets from eyes of the older rats we used (9-15 days).…”

A new wound healing model of retinal pigment epithelial cells in sheet culture

“……”

Ganglion cell loss in RCS rat retina: A result of compression of axons by contracting intraretinal vessels linked to the pigment epithelium

“…The contractile filaments myosin I and II have also been detected in the RPE cells of various mammals and fish (Breckler and Burnside, 1994). The presence of actin in RPE cells has been reported in numerous in vitro and in vivo studies (e.g., Burnside, 1976;Burnside and Laties, 1979;Chaitin and Hall, 1983;Haley et al, 1983;Owaribe, 1988;Fuchs et al, 1991) and has been shown to reorganise during migration (e.g., Campochiaro and Glaser, 1986) and regeneration (Korte et al, 1995). Lastly, some in vitro studies have shown that the interaction of RPE cells with matrix may cause contraction of the RPE cell sheets (Verstraeten et al, 1992;Smith-Thomas et al, 1996), and RPE cells have also been shown to mediate collagen contraction in vitro (Yamakawa et al, 1989;Raymond and Thompson, 1990;Grisanti and Guidry, 1995).…”