The cytoplasmic linker protein CLIP-170 is a human autoantigen

Griffith, Kevin J.; Ryan, J; Senécal, J-L; Fritzler, Marvin J.

doi:10.1046/j.1365-2249.2002.01756.x

Cited by 22 publications

(12 citation statements)

References 34 publications

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“…CLIP-170 enables the interaction between endosomal vesicles and cytoplasmic microtubules [46]. Although further studies are being conducted in large cohorts of patients with autoimmune diseases, anti-CLIP-170 antibodies may identify a subset of patients with CREST syndrome who are ANA-and ACA-negative.…”

Section: Novel Autoantibodiesmentioning

confidence: 99%

Antibodies in scleroderma: Direct pathogenicity and phenotypic associations

Chung

Utz²

2004

Curr Rheumatol Rep

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Scleroderma is an autoimmune disease involving endothelial cell damage and fibroblast overproduction of extracellular matrix. Several autoantibodies present in the sera of patients with scleroderma, including anti-endothelial cell, antifibroblast, anti-matrix metalloproteinase, and antifibrillin-1 antibodies, may directly contribute to disease pathogenesis. Scleroderma also is characterized by the presence of antinuclear and antinucleolar antibodies, which correlate with particular phenotypes. These include antitopoisomerase-I, anticentromere, antihistone, anti-polymyositis/scleroderma, anti-Th/To, anti-U3-small nucleolar ribonucleoprotein particle, anti-U1-small nuclear ribonucleoprotein particle, anti-RNA polymerase, and anti-B23 antibodies. Other antibodies classically associated with other autoimmune diseases, such as antiphospholipid, antineutrophil cytoplasmic, and antimitochondrial antibodies, also have been described in patients with scleroderma. This review will summarize the various autoantibodies associated with scleroderma, their putative pathogenic roles, and their phenotypic correlations.

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Section: Novel Autoantibodiesmentioning

confidence: 99%

Antibodies in scleroderma: Direct pathogenicity and phenotypic associations

Chung

Utz²

2004

Curr Rheumatol Rep

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“…Other high molecular weight autoantigens with similar features have been reported in cytoplasmic and mitotic organelles suggesting that these selected proteins become autoimmunogenic based on their subcellular association and molecular features [41]. For example, in the endosomal compartment, the two known autoantigens are early endosomal protein EEA1 (180 kDa) [44] and CLIP-170 (170 kDa) [45]. There is also a series of centrosomal autoantigens identified as coiled-coil-rich proteins including pericentrin, a 220 kDa protein [46], ninein, a protein with alternatively spliced products of 245 and 249 kDa [47], Cep250 (250 kDa) and Cep110 (110 kDa) [48].…”

Section: Resultsmentioning

confidence: 97%

Untitled

Chen³

et al. 2005

Arthritis Res Ther

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There has been some evidence that Behçet's disease (BD) has a significant autoimmune component but the molecular identity of putative autoantigens has not been well characterized. In the initial analysis of the autoantibody profile in 39 Chinese BD patients, autoantibodies to cellular proteins were uncovered in 23% as determined by immunoblotting. We have now identified one of the major autoantibody specificities using expression cloning. Serum from a BD patient was used as a probe to immunoscreen a λZAP expression cDNA library. Candidate autoantigen cDNAs were characterized by direct nucleotide sequencing and their expressed products were examined for reactivity to the entire panel of BD sera using immunoprecipitation. Reactivity was also examined with normal control sera and disease control sera from patients with lupus and Sjögren's syndrome. Six independent candidate clones were isolated from the cDNA library screen and were identified as overlapping partial human kinectin cDNAs. The finding that kinectin was an autoantigen was verified in 9 out of 39 (23%) BD patient sera by immunoprecipitation of the in vitro translation products. Sera from controls showed no reactivity. The significance of kinectin as a participant in autoimmune pathogenesis in BD and the potential use of autoantibody to kinectin in serodiagnostics are discussed.

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“…This is interesting since we and others reported a class of coiled-coil proteins that are recognized as intracellular autoantigens in systemic autoimmune diseases (12,33). Coiled-coil rich autoantigens have been noted in several cytoplasmic organelles, including a family of Golgi complex antigens known as golgins (12,33), endosomal proteins EEA1 (34) and CLIP-170 (35), centrosomal proteins pericentrin (36), ninein (37), and Cep250 and Cep110 (38). The mitotic organelles are also known to be associated with coiled-coil rich autoantigens, including NuMA (10,11,39), and centromere associated protein CENP-E (38,40,41) and CENP-F (42,43).…”

Section: Discussionmentioning

confidence: 99%

Autoantibody to NA14 is an independent marker primarily for Sjögren's syndrome

Nozawa

Ikeda²,

Satoh³

et al. 2009

Front Biosci

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Nuclear Autoantigen of 14 kDa (NA14) was originally identified using the serum of a Sjögren’s syndrome (SS) patient as probe in screening a human testis cDNA expression library. To date there is no report in the systematic analysis of the prevalence of autoantibodies to NA14. In this study, anti-NA14 was determined in several rheumatic diseases from independent cohorts in the US and Japan. The prevalence of anti-NA14 were 18/132 (13.6%) in primary SS, 0/50 (0%) secondary SS, 2/100 (2%) SLE, 1/43 (2.3%) scleroderma, 0/54 (0%) rheumatoid arthritis, 1/29 (3.4%) polymyositis/dermatomyositis, and 0/58 (0%) normal healthy controls. The frequencies of anti-NA14 positive sera in primary SS are statistically greater than normal healthy controls (p=0.006), secondary SS (p=0.044), and other rheumatic diseases. Furthermore, among 11 anti-NA14 positive primary SS sera, 4/11 (36.3%) sera were negative for both anti-SS-A/Ro and SS-B/La antibodies. Thus anti-NA14 autoantibodies may be useful for the discrimination of primary versus secondary SS and serve as a diagnostic marker for primary SS especially in seronegative (anti-SS-A/Ro and anti-SS-B/La antibodies negative) patients with SS.

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The cytoplasmic linker protein CLIP-170 is a human autoantigen

Cited by 22 publications

References 34 publications

Antibodies in scleroderma: Direct pathogenicity and phenotypic associations

Antibodies in scleroderma: Direct pathogenicity and phenotypic associations

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Autoantibody to NA14 is an independent marker primarily for Sjögren's syndrome

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