The cytology of extraskeletal Ewing sarcoma

Guiter, Gerardo; M, Gamboni; Zakowski, Maureen F.

doi:10.1002/(sici)1097-0142(19990625)87:3<141::aid-cncr7>3.0.co;2-c

Cited by 55 publications

(22 citation statements)

References 24 publications

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“…Histologically, this tumor is characterized by spherical to polygonal cells with round to oval nuclei and glycogen content [26,31,52]. The described immunohistochemistry varies, although immunoreactivity to CD99, S-100, and vimentin, with absence of cytokeratin and desmin, has been noted [9,25,30]. FISH and RT-PCR analyses reveal the fusion gene EWS-FL1 [t(11;22)(q24;q 12)] is present in greater than 80% of cases and an EWS-ETS fusion gene in most others.…”

Section: Discussion and Treatmentmentioning

confidence: 99%

Orthopaedic Case of the Month: A 14-Year-Old Boy With a Medial Thigh Soft Tissue Mass

Ibrahim

Mascarenhas

Tovar

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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Section: Discussion and Treatmentmentioning

confidence: 99%

Orthopaedic Case of the Month: A 14-Year-Old Boy With a Medial Thigh Soft Tissue Mass

Ibrahim

Mascarenhas

Tovar

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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“…Both of these tumors also may present as primary soft tissue tumors, however, very rarely (15)(16)(17). In children, intraosseous lymphoblastic lymphoma comprises a significant percentage of the lymphoma cases and is often misdiagnosed.…”

Section: Discussionmentioning

confidence: 99%

Differentiating Lymphoblastic Lymphoma and Ewing's Sarcoma: Lymphocyte Markers and Gene Rearrangement

et al. 2001

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We encountered a child with an intraosseous small round cell tumor that was negative for LCA, CD20 (L26), and CD3 and positive for vimentin, CD99 (MIC-2), and periodic acid-Schiff. The tumor exhibited rosette-like formations. This case was initially interpreted as Ewing's sarcoma (ES); however, additional studies revealed positivity for CD79a, CD43, and TdT expression, and an immunoglobulin heavy chain gene rearrangement (IgH-R) by polymerase chain reaction (PCR) established this to be a precursor B-lymphoblastic lymphoma. Because the differential diagnosis of ES and lymphoblastic lymphoma can be difficult and the differential diagnostic value of leukocyte antigens and immunoglobulin heavy chain gene rearrangement studies have not been fully evaluated, we conducted a more extensive investigation on 33 (21 soft tissue and 12 intraosseous) ES cases. Cases were retrieved from the files of the Department of Pathology at Georgetown University and from the Soft Tissue Registry of the Armed Forces Institute of Pathology. The cases were studied by light microscopy, immunohistochemistry, and PCR for IgH-R and T cell receptor gamma chain gene rearrangement (T␥-R). There were 17 females and 16 males; the mean age was 29.3 years. Locations included the extremities (n ‫؍‬ 17) and trunk (n ‫؍‬ 16). All cases fit the ES spectrum by light microscopy and immunohistochemistry, as previously determined, and were negative for lymphoid markers (LCA, CD3, CD20, CD43, CD79a, and TdT), CD10 and CD34. CD99 was positive in 31/33 and bcl-2 was weakly positive in 13/33 cases. All 21 cases studied for gene rearrangements by PCR were negative for IgH-R and T␥-R. Distinction of intraosseous lymphoblastic lymphoma from ES may be difficult because lymphomas may occasionally exhibit unexpected morphologic and immunophenotypic properties including LCA, CD3 and CD20 negativity and cytokeratin positivity. Additional analysis using CD79a, CD43, TdT, and PCR should be performed to avoid misdiagnosis. True ES is negative for lymphoid markers including CD79a, CD43, and TdT, as well as for IgH-R and T␥-R.

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“…This tumour shares histologic, immunohistochemical, and molecular findings with ES of bone [5]. The classical histologic description of Extraskeletal Ewing sarcoma is of a tumour composed of small, uniform, round or oval cells in solid sheets divided by fibrous strands.…”

Section: To the Editormentioning

confidence: 99%

“…Its cytoplasm is scanty, pale staining, and vacuolated because of the presence of glycogen, and the nuclei are round with ''salt and pepper'' chromatin and small nucleoli. A sensitive and relatively specific antigen, CD99/ MIC2, and a characteristic chromosomal translocation, t (11; 22), have been identified in skeletal ES and EES [5].…”

Section: To the Editormentioning

confidence: 99%