The culture and differentiation of amniotic stem cells using a microfluidic system

Wu, Huei Wen; Lin, Xi Zhang; Hwang, Shiaw Min; Lee, Gwo‐Bin

doi:10.1007/s10544-009-9304-x

Cited by 20 publications

(20 citation statements)

References 39 publications

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“…Consequently, the mechanism of changed pathways mentioned above may lead to a similar result (inhibition of adipogenesis) under mechanical stimulation (e.g., shear stress and pulsation frequency), as in this study. Similar to the results reported in the previous works (Yang et al 2006;Park et al 2007a;Wu et al 2009), MSCs may differentiate into chondrocyte or osteoblast instead of adipocytes in the purposed chip if proper conditions are applied. Therefore, the purposed chip realized a tunable dilution device (i.e., insulin concentration) integrated with a mechanical module which can provide different pressures simultaneously on adipogenic differentiation, which explored a quantitative assessment on adipogenesis of hMSC.…”

Section: Mechanical Stimulation Of Mscssupporting

confidence: 88%

A microfluidic device for chemical and mechanical stimulation of mesenchymal stem cells

Lin

Hwang

et al. 2011

Microfluid Nanofluid

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Recently, there has been considerable interest in stem cells which have the potential to differentiate into multiple lineages for cell therapy. Special attention has been paid, in particular, to the use of alternative sources of stem cells with fewer ethical issues. For example, mesenchymal stem cells (MSCs) from bone marrow have been proven to be multipotent for transplantation and tissue engineering. In this study, an integrated microfluidic chip capable of chemically and mechanically stimulating human mesenchymal stem cells (hMSCs) for adipogenic differentiation was presented. It was composed of a dilution module for controlling the insulin concentrations, and pneumatically-modulated membrane structures for precisely applying shear stresses on cells to perform chemical and mechanical stimulation, respectively. With this approach, a long-term culture and differentiation of MSCs were performed in an automatic manner. The accumulation of lipids that represented the results of insulin simulation was evaluated by Oil Red O staining. The measurements including lipid droplet numbers, optical density (OD) values, and expression of the PPARc2 gene were used to assess the level of differentiation of the MSCs. The experimental result showed that the maximum oil droplets were induced under an optimal insulin concentration of 10 lg/ml. It was also revealed that, under mechanical stimulation, adipogenesis was inhibited under stronger levels of applied shear stresses and at higher pulsation frequencies. This proposed microfluidic chip has great potential as a powerful tool for MSC studies.

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Section: Mechanical Stimulation Of Mscssupporting

confidence: 88%

A microfluidic device for chemical and mechanical stimulation of mesenchymal stem cells

Lin

Hwang

et al. 2011

Microfluid Nanofluid

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“…Spatial control of the proliferation and differentiation of NSCs was achieved by controlling gradients of growth factors in microfluidic devices [91] . An automatic microfluidic system for adipogenic and osteogenic differentiation of human AFSCs has been developed using micro-electro-mechanical-systems (MEMS) technology [92] . Neuronal and oligodendrocytic differentiation of NSC was significantly enhanced by 3-D microenvironments in microfluidic channels, suggesting that mimicking in vivo microenvironment should promote NSC differentiation [93] .…”

Section: Applications In Stem Cell Differentiation and Drug Screeningmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as "niches", to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix (synthetic and decellularized), paracrine/ autocrine signaling and physical forces (i.e. , mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general, bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.

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“…25 Typically, a streamline-shaped design in microfluidic devices has been adopted so that fluids flow smoothly to address this problem. 26 In this review, recent advancements in cell studies, especially for stem cells, in microfluidic systems are reviewed. Advances in microfluidic systems for stem cell study will be summarized and discussed.…”

Section: A Microfluidicsmentioning

confidence: 99%

“…Different methods for applications with MSC are described in this section, including microenvironments built for MSC culture and differentiation, 26,[140][141][142][143] …”

Section: B Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

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The culture and differentiation of amniotic stem cells using a microfluidic system

Cited by 20 publications

References 39 publications

A microfluidic device for chemical and mechanical stimulation of mesenchymal stem cells

A microfluidic device for chemical and mechanical stimulation of mesenchymal stem cells

Engineering stem cell niches in bioreactors

Stem cells in microfluidics

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