The creation of new aneuploid lines of the cotton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR-markers

Sanamyan, M. F.; Bobokhujaev, Sh. U.; Makamov, Abdusalom K.; Achilov, S. G.; Abdurakhmonov, Ibrokhim Y.

doi:10.18699/vj16.186

Cited by 3 publications

(6 citation statements)

References 19 publications

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“…Since six translocation lines share one common chromosome 2, then the univalent chromosome lines in monosome Mo11, Mo16, Mo19, and Mo93 are chromosome 2 of the A t subgenome of cotton and, as four monosomic lines are duplicates. Molecular genetic analysis of four monosomic interspecific F 1 hybrids with the participation of the lines Mo11, Mo16, Mo19 and Mo93 confirmed these data (Sanamyan et al, 2016).…”

Section: Resultssupporting

confidence: 60%

“…calization of chromosome-specific SSR markers on F 1 hybrids involving the lines Mo7, Mo38, Mo58, Mo59, Mo60, Mo69, Mo71, and Mo81 confirmed this data(Sanamyan et al, 2016).…”

supporting

confidence: 69%

“…Therefore, to determine which of the two chromosomes of this translocation, the homologous univalent chromosome of the Mo48 monosomic line was used earlier, we used chromosome-specific microsatellite SSR markers that were amplified by standard PCR. Moleculargenetic analysis of the monosomic interspecific hybrid F 1 (Mo48×Pima 3-79) revealed the presence of polymorphic alleles only from the species Gossypium barbadense L., which indicated the localization of the specific SSR marker BNL3280 chromosomes in the aforementioned hybrid (Sanamyan et al, 2016). Since this marker was previously located on the chromosome of the 18 D t -subgenome of cotton, we can assume that the monosomic line -Mo48 of the collection of NUUs has a monosomy along the chromosome of the 18 D t -subgenome.…”

Section: Resultsmentioning

confidence: 99%

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Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Sanamyan

Bobokhujayev

2019

Vestn. VOGiS

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The lack of clear morphological markers of cotton chromosomes contributed to the development of an unconventional method for marking chromosomes using translocations. Today, tester translocation cotton lines represent the most complete set of cytological markers. The results of cytogenetic analysis of F1 hybrids obtained from crosses of monosomic cotton lines with translocation lines with identified chromosomes are presented. Cytogenetic identification and numbering of univalent chromosomes in 25 monosomic lines of the cytogenetic collection of the National University of Uzbekistan allowed us to establish the following univalent occurrences: chromosome 2 in four monosomic lines, chromosome 4 in 15 lines, chromosome 6 in four lines, chromosome 7 of the At-subgenome in one line and chromosome 18 of the Dt-subgenome in one line. The remaining 21 lines were duplicates of three non-homologous chromosomes. All monosomic lines identified were characterized by differences in univalent sizes, meiotic index, number of tetrads with micronuclei, pollen fertility, frequency of monosomy in the progeny, and a complex of morphological characters associated with the monosomy of the chromosome identified. Despite differences in the genotypic environment and methods for producing monosomics in the two cotton collections, there is a surprising coincidence of data suggesting a higher frequency of chromosomes 2, 4 and 6 occurring as monosomics, while the other chromosomes of the set occur as monosomics at a much lower frequency, and eight nonhomologous chromosomes (5, 8, 13 of the At-subgenome and 14, 15, 19, 22 and 24 of the Dt-subgenome of cotton) never do.

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Section: Resultssupporting

confidence: 60%

“…calization of chromosome-specific SSR markers on F 1 hybrids involving the lines Mo7, Mo38, Mo58, Mo59, Mo60, Mo69, Mo71, and Mo81 confirmed this data(Sanamyan et al, 2016).…”

supporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

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Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Sanamyan

Bobokhujayev

2019

Vestn. VOGiS

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Section: Introductionmentioning

confidence: 99%

“…Previously, we obtained new monosomic cotton lines in a single genotypic background of the highly inbred G. hirsutum L. line L-458 (A t D t genome) [27][28][29][30]; these lines resulted in long-term inbreeding and selection with the cotton cultivar 108-F. Univalent chromosomes of these lines were identified using a well-defined tester set of cotton translocation lines (USA) [31,32]. The identification of most monosomic lines was confirmed using SSR markers at the Center for Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan [30,31]. As a result, four monosomic lines from the Cytogenetic Collection of Uzbekistan (Mo11, Mo16, Mo19, and Mo93) were found to have chromosome 2 of the A t subgenome; 16 monosomic lines (Mo7, Mo31, Mo38, Mo58, Mo59, Mo60, Mo69, Mo70, Mo71, Mo72, Mo73, Mo75, Mo76, Mo79, Mo81, and Mo89) had chromosome 4 of the A t subgenome; five monosomic lines (Mo13, Mo34, Mo67, Mo92, and Mo95) had chromosome 6 of the A t subgenome; Mo27 had chromosome 7 of the A t subgenome; Mo48 line had chromosome 18 of the D t subgenome; and monotelodisomic line Mo21 lacks an arm of chromosome 11 of the A t subgenome [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Features of Chromosome Introgression from Gossypium barbadense L. into G. hirsutum L. during the Development of Alien Substitution Lines

Sanamyan

Bobohujayev

Abdukarimov³

et al. 2022

Plants

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The creation of G. barbadense L./G. hirsutum L. chromosome-substitution linesis an important method to transfer agronomically valuable traits from G. barbadense into G. hirsutum. In this study, 30 monosomic lines of G. hirsutum from the Cytogenetic Collection of Uzbekistan, created in the genotypic background of line L-458, were used in crosses with the G. barbadense line Pima 3-79 to create substitution lines. In the course of this work, new monosomic lines were identified for chromosome 12 and monotelodisome 6 of the Atsubgenome and for chromosomes 17, 21, and 22 of the Dtsubgenome using chromosome-specific SSR markers and a well-defined tester set of cotton translocation lines (USA). Compared to those in the F1 hybrids, a strong decrease in the crossing and setting rates was found in the BC1F1 backcross lines, with the substitution of chromosomes 2, 4, 6, 7, and 12 of the Atsubgenome and 17, 18, 21, and 22 of the Dtsubgenome. The F1 and BC1F1 offspring from interspecific crosses differed in their transmission of univalents. Despite the regular pairing of chromosomes and the high meiotic index, interspecific aneuploid hybrids were characterized by a decrease in pollen fertility, which may indicate hidden structural variability in these genomes that did not affect meiotic division. The identification of chromosomes using chromosome-specific SSR markers in the early stages of plant development has greatly accelerated the detection of monosomic plants. The analysis of morphobiological traits revealed that monosomic F1 hybrids were more similar to the donor line, while BC1F1 hybrids were more similar to the recurrent parent but also showed previously undetected traits.

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Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from Gossypium barbadense L. into the genome of G. hirsutum L. in BC2F1 hybrids

Sanamyan,

Bobokhujayev,

Abdukarimov

et al. 2023

Vestn. VOGiS

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Substitution lines of the cotton Gossypium hirsutum L. involving chromosomes of the tetraploid species G. bar ba dense L., G. tomentosum Nutt. ex Seem., and G. mustelinum Miers ex Watt. are a valuable source for breeding, increasing the genetic diversity of G. hirsutum. The substitution of certain G. hirsutum L. chromosomes with G. barbadense chromosomes affect fibre elongation, fibre yield, fibre strength, and micronaire. To increase the efficiency of creating lines, it is necessary to study the nature of the introgression of alien chromosomes into the G. hirsutum L. genome. As a result of molecular genetic analysis of BC2F1 hybrids obtained from crossing monosomic lines of the cotton G. hirsutum from the cytogenetic collection of Uzbekistan with monosomic backcross hybrids BC1F1 G. hirsutum × G. barbadense on the same chromosomes, genetic differences between the hybrids in the profile of chromosome-specific microsatellite SSR markers were found. The predominant introgression of chromosomes 4, 6 and 12 of the At-subgenome and 22 of the Dt-subgenome of G. barbadense was revealed, while chromosomes 2 and 7 of the At-subgenome and 18 of the Dt- subgenome of G. barbadense were characterized by elimination. Among them, chromosomes 7 of the At- sub genome and 18 of the Dt-subgenome of G. barbadense were eliminated in the first backcross generation. In this work, two lines, CS- B06 and CS-B07, from the American cytogenetic collection with a putative substitution involving chromosomes 6 and 7 of the At-subgenome were analysed. The presence of only polymorphic alleles from the species G. hirsutum and the absence of polymorphic alleles from the species G. barbadense were revealed, which showed the absence of substitution involving these chromosomes. BC2F1 hybrids with monosomy for both G. barbadense and G. hirsutum chromosomes were characterized by regular pairing of chromosomes and high meiotic indexes. However, many hybrids were characterized by a decrease in pollen fertility. Two hybrids with monosomy for chromosome 7 of the At-subgenome of G. hirsutum and chromosome 6 of the At-subgenome of G. barbadense had the greatest reduction in pollen viability (70.09 ± 1.57 and 75.00 ± 1.66 %, respectively). Thus, this work shows a specific feature in the introgression of individual chromosomes of the cotton species G. barbadense into the cotton G. hirsutum genome.

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The creation of new aneuploid lines of the cotton (Gossypium hirsutum L.) with identification of chromosomes by translocation and SSR-markers

Cited by 3 publications

References 19 publications

Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Identification of univalent chromosomes in monosomic lines of cotton (Gossypium hirsutum L.) by means of cytogenetic markers

Features of Chromosome Introgression from Gossypium barbadense L. into G. hirsutum L. during the Development of Alien Substitution Lines

Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids

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