The CR mutation and catabolite repression in Escherichiacoli

Rickenberg, H.V.; Hsie, Abraham W.; Janeček, J.

doi:10.1016/0006-291x(68)90521-4

Cited by 23 publications

(9 citation statements)

References 6 publications

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“…It must be noted, however, that the role of the CR gene has recently been questioned. Rickenberg et al (27) obtained evidence that the CR gene effects glucose metabolism rather than conferring specific sensitivity on the lac operon to catabolite repression as postulated by Loomis and Magasanik (9). Until this point is clarified, the existence of the CR gene and the CR circuit must be viewed with the following alternatives in mind.…”

Section: Discussionmentioning

confidence: 99%

Corepressor System for Catabolite Repression of the lac Operon in Escherichia coli

Dobrogosz

1969

J Bacteriol

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Acetylated amino sugars, normally used in the biosynthesis of cell walls and cell membranes, were found to play a role as corepressors for catabolite repression of the lac operon in Escherichia coli. This conclusion was derived from studies conducted on mutants of E. coli that were able to assimilate an exogenous source of Nacetylglucosamine (AcGN) but were unable to dissimilate or grow onthis compound. At concentrations less than 1O-4 M, AcGN caused severe catabolite repression of f3-galactosidase synthesis in cultures grown under either nonrepressed or partially repressed conditions. This repression occurred in the absence of any effect of AcGN on either the carbon and energy metabolism or the growth of the organism. In addition, this repression by AcGN occurred in a mutant strain that is constitutive for ,3-galactosidase production, demonstrating that the AcGN effect does not involve the uptake of inducer. This model for the corepressor system of catabolite repression is discussed in relation to the existing theories on repression of the lac operon. Chemicals. Isopropyl- ,-D-thiogolactopyranoside (IPTG), O-nitrophenyl-B-D-galactopyranoside (ONPG), and AcGN were obtained from the Mann Research Laboratories, New York, N.Y. Uniformnly labeled 14C-leucine, D-gluconate-1-14C, and D-glucosamine-1-14C were obtained from the New England Nuclear Corp., Boston, Mass. AcGN-1-'4C was prepared by acetylation of glucosamine-1-'4C by the procedure of Roseman and Ludoweig (28). Purity of the compound was checked by paper chromatography with n-butyl alcohol-pyridine-water (6:4:3). All other chemicals were of reagent grade and are readily available. Cultures and growth conditions. Most of the strains used in this study were derived from E. coli K-12-700 and were kindly made available by R. J. White (Oxford University, Great Britain). A complete description of these cultures, including the methods used for isolation of the mutant strains, is given elsewhere (29). From the parental strain, two mutants were obtained: one, K-12-1-1, is a mutant lacking the enzyme glucosa-1083

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Section: Discussionmentioning

confidence: 99%

Corepressor System for Catabolite Repression of the lac Operon in Escherichia coli

Dobrogosz

1969

J Bacteriol

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“…J. Janeček took part in the pioneering work which proved that Escherichia coli mutants resistant to catabolic repression have defects in degradation of cyclic AMP [142,182].…”

Section: General Microbiologymentioning

confidence: 99%

General and molecular microbiology and microbial genetics in the IM CAS

Nešvera

2010

J Ind Microbiol Biotechnol

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This review summarizes the main results obtained in the fields of general and molecular microbiology and microbial genetics at the Institute of Microbiology of the Academy of Sciences of the Czech Republic (AS CR) [formerly Czechoslovak Academy of Sciences (CAS)] over more than 50 years. Contribution of the founder of the Institute, academician Ivan Málek, to the introduction of these topics into the scientific program of the Institute of Microbiology and to further development of these studies is also included.

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“…It was shown by RICKENBERG et al (18) that CR-mutant isolated by LooMIS and MAGASANIK (19,20) was not subject to the repression of f3-galactosidase synthesis by glucose, but was by glucose plus gluconate or glucose 6-phosphate (G-6-P), suggesting that this CR-strain was mutated concerning the metabolism of glucose. Such mutation profiles were then examined for tryptophanase formation when the strain TAB40-203 was grown on glucose plus gluconate or G-6-P as a carbon source.…”

Section: Effect Of Other Carbon Sources On Tryptophanase Formation Inmentioning

confidence: 99%

An Escherichia Coli Mutant With Tryptophanase Synthesis Specifically Insensitive to Catabolite Repression and the Significance of Intracellular Phosphoenolpyruvate Pools

Sato

Aida

Uemura

1974

J. Gen. Appl. Microbiol.

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The CR mutation and catabolite repression in Escherichiacoli

Cited by 23 publications

References 6 publications

Corepressor System for Catabolite Repression of the lac Operon in Escherichia coli

Corepressor System for Catabolite Repression of the lac Operon in Escherichia coli

General and molecular microbiology and microbial genetics in the IM CAS

An Escherichia Coli Mutant With Tryptophanase Synthesis Specifically Insensitive to Catabolite Repression and the Significance of Intracellular Phosphoenolpyruvate Pools

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