The correlation between gene expression of proinflammatory markers and bone formation during osseointegration with titanium implants

Omar, Omar; Lennerås, Maria; Suska, Felicia; Emanuelsson, Lena; Hall, Jan; Palmquist, Anders; Thomsen, Peter

doi:10.1016/j.biomaterials.2010.09.011

Cited by 72 publications

(106 citation statements)

References 20 publications

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“…In vivo studies have shown that implants with combined nanoscale and microscale roughness enhance the long-term bone response 2 and promote bone-bonding and interface strength. [5][6][7] However, conclusive understanding of the molecular mechanisms underlying the cell and tissue interactions with nanoscale topography, per se, is still lacking. This is, in part, due to the difficulty in isolating particular effects of nanotopography from other surface properties (eg, microtopography and surface chemistry).…”

Section: Introductionmentioning

confidence: 99%

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

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Purpose Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/µm 2 ). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α , and the osteoclastic marker, CatK , were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory ( TNF -α) and osteoclastic ( CatK ) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

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Section: Introductionmentioning

confidence: 99%

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…In our study, prosthesis characteristics (i.e., type of fixation) could not explain the variability in response to osteogenic stimuli. However, other studies showed that surface characteristics of implants can influence the inflammatory response to implant material and subsequently affect the expression of bone formation markers (e.g., BMP‐2 and ALP) 46, 47. Therefore, in future studies, the effect of different implant materials and surface characteristics on osteogenic differentiation of peri‐prosthetic tissue cells should be investigated.…”

Section: Discussionmentioning

confidence: 99%

Peri-prosthetic tissue cells show osteogenic capacity to differentiate into the osteoblastic lineage

et al. 2017

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During the process of aseptic loosening of prostheses, particulate wear debris induces a continuous inflammatory‐like response resulting in the formation of a layer of fibrous peri‐prosthetic tissue at the bone‐prosthesis interface. The current treatment for loosening is revision surgery which is associated with a high‐morbidity rate, especially in old patients. Therefore, less invasive alternatives are necessary. One approach could be to re‐establish osseointegration of the prosthesis by inducing osteoblast differentiation in the peri‐prosthetic tissue. Therefore, the aim of this study was to investigate the capacity of peri‐prosthetic tissue cells to differentiate into the osteoblast lineage. Cells isolated from peri‐prosthetic tissue samples (n = 22)−obtained during revision surgeries−were cultured under normal and several osteogenic culture conditions. Osteogenic differentiation was assessed by measurement of Alkaline Phosphatse (ALP), mineralization of the matrix and expression of several osteogenic genes. Cells cultured in osteogenic medium showed a significant increase in ALP staining (p = 0.024), mineralization of the matrix (p < 0.001) and ALP gene expression (p = 0.014) compared to normal culture medium. Addition of bone morphogenetic proteins (BMPs), a specific GSK3β inhibitor (GIN) or a combination of BMP and GIN to osteogenic medium could not increase ALP staining, mineralization, and ALP gene expression. In one donor, addition of GIN was required to induce mineralization of the matrix. Overall, we observed a high‐inter‐donor variability in response to osteogenic stimuli. In conclusion, peri‐prosthetic tissue cells, cultured under osteogenic conditions, can produce alkaline phosphatase and mineralized matrix, and therefore show characteristics of differentiation into the osteoblastic lineage. © 2016 The Authors. Journal of Orthopaedic Research published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 35:1732–1742, 2017.

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“…Therefore, a number of studies has been reported on improving of implant body surface topography and chemistry for shortening the period of establish osseointegration, and increase the success rate of implant in these patients [2][3][4] . On the other hands, some studies were reported about methods of improving the jaw bone as host side.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Angiogenesis in Bone Defect Healing Process due to the Difference in the Frequency of Low-Intensity Pulsed Ultrasound (LIPUS)

Sasaki

Monden

Yoshinari

et al. 2016

J. Hard Tissue Biology.

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Low-intensity pulsed ultrasound (LIPUS) is known to promote bone defect healing and also angiogenesis. It was reported the frequency of the LIPUS was related to directivity and the depth of penetration, but the differences in angiogenesis during bone defected healing remains unknown. The aim of this study is to investigate the effect of low and high frequency LIPUS exposure for the angiogenesis during rat femur bone defect healing process by molecular biological and histomorphological evaluations. Bone defects of 1.6 mm in diameter were created in both femurs of ten-week-old male Long-Evans rats (n=30). Right femur as LIPUS exposure groups were exposed LIPUS (intensity: 30 mW/cm2, burst width: 200 µs, time: 15 min/ day) and divided into a low frequency (1.5 MHz, L15) group and a high frequency (3.0 MHz, L30) group. Left femur as non-exposed LIPUS group were used as control. After 3, 5, and 7 days, femurs were removed and quantitative RT-PCR (qRT-PCR) for vascular endothelial growth factor (VEGF), histomorphological and immunohistochemical evaluations and measurement of new formed capillary vessel ratio in bone defected are were conducted. The results of qRT-PCR were indicated that VEGF expression of L15 at 5 days was significantly higher than that of L30 and control group (p < 0.05). Immune-positive reaction of VEGF was recognized in fibroblasts, endothelial cells, periosteal cells and osteoblast and these expression in LIPUS exposure groups were stronger than control groups. The capillary vessel formation ratio in upper layer of bone defected area in L15 group was significantly increased compared to L30 and control group at 7 days (p < 0.05). In conclusion, 1.5 MHz frequency of LIPUS exposure was more effective to promote VEGF expression and angiogenesis than 3.0 MHz in rat femur bone defected healing.

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The correlation between gene expression of proinflammatory markers and bone formation during osseointegration with titanium implants

Cited by 72 publications

References 20 publications

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Peri-prosthetic tissue cells show osteogenic capacity to differentiate into the osteoblastic lineage

Comparison of Angiogenesis in Bone Defect Healing Process due to the Difference in the Frequency of Low-Intensity Pulsed Ultrasound (LIPUS)

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The correlation between gene expression of proinflammatory markers and bone formation during osseointegration with titanium implants

Cited by 72 publications

References 20 publications

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

Peri-prosthetic tissue cells show osteogenic capacity to differentiate into the osteoblastic lineage

Comparison of Angiogenesis in Bone Defect Healing Process due to the Difference in the Frequency of Low-Intensity Pulsed Ultrasound (LIPUS)

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The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo