The correlation between antibiotic resistance gene abundance and microbial community resistance in pig farm wastewater and surrounding rivers

Yang, Yiwen; Liu, Zixiao; Xing, Si-Cheng; Liao, Xindi

doi:10.1016/j.ecoenv.2019.109452

Cited by 41 publications

(15 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Physiochemical parameters such as BOD, COD and SS were positively correlated (p < 0.05) with the majority of ARGs in WWTP influents, while negative correlations were observed in effluents. Previous studies reported that pH, COD, and TN/P were positively correlated with ARG abundance in both influent and effluent from municipal WWTPs, and they may be important factors affecting the bacterial community during the treatment process [41,42]. Because raw influent wastewater contains massive amounts of organics and excess nutrients compared to effluent, potentially providing selective pressure for ARB and ARGs [6,12], physiochemical parameters may be positively correlated with major ARG types.…”

Section: Diversity and Occurrence Of Args From Metagenomic Analysismentioning

confidence: 99%

“…These results suggested that ARG abundance may not be affected by the WWTP scale, but could be affected by influent characteristics in WWTPs. Some previous studies have shown that selected physiochemical parameters are often weakly correlated with ARG abundance in WWTPs, and abundance of ARGs does not rely on operation conditions such as temperature, HRT, SRT and MLSS [41][42][43][44]. Therefore, more studies are required to resolve and determine the underlying physicochemical, biological, and operating drivers for the dissemination of ARG patterns between large and small WWTPs.…”

Section: Diversity and Occurrence Of Args From Metagenomic Analysismentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Yoo

Lee

2021

Antibiotics

View full text Add to dashboard Cite

Although extensive efforts have been made to investigate the dynamics of the occurrence and abundance of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs), understanding the acquisition of antibiotic resistance based on the WWTP scale and the potential effects on WWTPs is of relatively less interest. In this study, metagenomic analysis was carried out to investigate whether the WWTP scale could be affected by the prevalence and persistence of ARGs and mobile genetic elements (MGEs). As a result, 152 ARG subtypes were identified in small-scale WWTP samples, while 234 ARG subtypes were identified in large-scale WWTP samples. Among the detectable ARGs, multidrug, MLS (macrolide–lincosamide–streptogramin), sulfonamide, and tetracycline resistance genes had the highest abundance, and large and small WWTPs had similar composition characteristics of ARGs. In MGE analysis, plasmids and integrons were 1.5–2.0-fold more abundant in large-scale WWTPs than in small-scale WWTPs. The profile of bacteria at the phylum level showed that Proteobacteria and Actinobacteria were the most dominant bacteria, representing approximately 70% across large- and small-scale WWTPs. Overall, the results of this study elucidate the different abundances and dissemination of ARGs between large- and small-scale WWTPs, which facilitates the development of next-generation engineered wastewater treatment systems.

show abstract

Section: Diversity and Occurrence Of Args From Metagenomic Analysismentioning

confidence: 99%

Section: Diversity and Occurrence Of Args From Metagenomic Analysismentioning

confidence: 99%

Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Yoo

Lee

2021

Antibiotics

View full text Add to dashboard Cite

show abstract

“…Although these superbugs are mostly found in the hospital environment, current data has shown that these bacteria can spill over from their anthropogenic sources into natural ecosystems where they can create secondary reservoirs leading to the spread of these bacteria and their resistance determinants through the environment [ 4 ]. Effluents from wastewater treatment plants, industry, hospitals and farms will eventually reach some water source making the aquatic environments a major pool for ARB and antimicrobial resistance genes (ARG) [ 5 , 6 , 7 , 8 ]. Water is the primary source by which ARB enter the natural ecosystems and it is also a vehicle of transportation of these bacteria.…”

Section: Introductionmentioning

confidence: 99%

Distribution and Clonal Diversity of Staphylococcus aureus and Other Staphylococci in Surface Waters: Detection of ST425-t742 and ST130-t843 mecC-Positive MRSA Strains

et al. 2021

View full text Add to dashboard Cite

Natural aquatic environments represent one of the most important vehicles of bacterial dissemination. Therefore, we aimed to isolate staphylococci from surface waters and to investigate the presence of antimicrobial resistance genes and virulence factors as well as the genetic lineages of all Staphylococcus aureus isolates. Staphylococci were recovered from water samples collected from 78 surface waters, including rivers, streams, irrigation ditches, dams, lakes, and fountains. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR. Multilocus sequence typing and spa-typing were performed in all S. aureus isolates. From the 78 water samples, 33 S. aureus, one S. pseudintermedius, and 51 coagulase-negative staphylococci (CoNS) were identified. Among the S. aureus isolates, four MRSA were identified, and all harbored the mecC gene. Fourteen S. aureus were susceptible to all antimicrobials tested and the remaining showed resistance to penicillin, erythromycin and/or tetracycline encoded by the blaZ, ermT, msr(A/B), tetL, and vgaA genes. Regarding the clonal lineages, one mecC-MRSA isolate belonged to spa-type t843 and sequence type (ST) 130 and the other three to t742 and ST425. The remaining S. aureus were ascribed 14 spa-types and 17 sequence types. Eleven species of CoNS were isolated: S. sciuri, S. lentus, S. xylosus, S. epidermidis, S. cohnii spp. urealyticus, S. vitulinus, S. caprae, S. carnosus spp. Carnosus, S. equorum, S. simulans, and S. succinus. Thirteen CoNS isolates had a multidrug resistance profile and carried the following genes: mecA, msr(A/B), mph(C), aph(3′)-IIIa, aac(6′)-Ie–aph(2′’)-Ia, dfrA, fusB, catpC221, and tetK. A high diversity of staphylococci was isolated from surface waters including mecCMRSA strains and isolates presenting multidrug-resistance profiles. Studies on the prevalence of antibiotic-resistant staphylococci in surface waters are still very scarce but extremely important to estimate the contribution of the aquatic environment in the spread of these bacteria.

show abstract

“…Culture wastewater does great harm to environment and water body. The treated piggery wastewater can effectively reduce the resistance of microbial antibiotic resistance genes and three antibiotics (ciprofloxacin, streptomycin, and tetracycline hydrochloride) in surrounding rivers (Yang et al,2019).…”

Section: Application Of Molecular Biology Methods In Pollution Controlmentioning

confidence: 99%

Molecular biological methods in environmental engineering

Zhang

et al. 2020

Water Environment Research

View full text Add to dashboard Cite

Microbes are sensitive to environmental changes and can respond in a short time. Genomics, proteomics, transcriptomics, metabolomics, and multigroup association are used to characterize the composition, function, and metabolism of microorganisms, and to evaluate the environment according to the changes in microorganisms, which has important reference and guiding significance of environmental monitoring, management, and repair. In this paper, the application of molecular biological methods to study environmental microorganisms in the fields of wastewater treatment, pollution control, soil improvement, and environmental monitoring in 2019 is reviewed.

show abstract

The correlation between antibiotic resistance gene abundance and microbial community resistance in pig farm wastewater and surrounding rivers

Cited by 41 publications

References 51 publications

Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Distribution and Clonal Diversity of Staphylococcus aureus and Other Staphylococci in Surface Waters: Detection of ST425-t742 and ST130-t843 mecC-Positive MRSA Strains

Molecular biological methods in environmental engineering

Contact Info

Product

Resources

About