The cooperative role of membrane skeleton and bilayer in the mechanical behaviour of red blood cells

Svetina, S.; Kuzman, Drago; Waugh, Richard E.; Ziherl, P.; Žekš, B.

doi:10.1016/j.bioelechem.2003.08.002

Cited by 67 publications

(49 citation statements)

References 27 publications

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“…[11][12][13][14][15][16][17] However, there are reports suggesting that the elastic properties of the lipid bilayer cell membrane could also be quite important for overall cell stiffness. 6,13,[18][19][20] In particular, membrane elasticity could be significantly affected by intercalation with specific molecules, such as amphiphilic molecules 6 and stress hormones. molecules and increasing intracellular concentration of adenosine triphosphate, decreased potassium ion flow away from the cell, an increase of the protein phosphorylation level, and, as a result, affecting erythrocyte membrane stability.…”

Section: Discussionmentioning

confidence: 99%

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Żuk¹,

Targosz‐Korecka²,

Szymoński³

2011

IJN

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Background The main function of red blood cells is to transport oxygen to all parts of the body with the help of hemoglobin. Other proteins of the cell membrane can attach xenobiotics (eg, drugs) from the blood and transport them throughout the body. Only drugs able to bind to the membrane of the red blood cell can modify its structure and elastic properties. The morphology and local elastic properties of living red blood cells incubated with drug solutions commonly used in the treatment of severe asthma were studied by atomic force microscopy and nanoindentation with an atomic force microscopy tip. Methods The elasticity modules of native red blood cells, as well as those incubated with two types of drugs, ie, aminophylline and methylprednisolone, were determined from experimentally measured nanoindentation curves. Results The elasticity modules of erythrocytes incubated with aminophylline were substantially higher than those obtained for nonincubated native, ie, healthy, red blood cells. Conclusion The increase of the elasticity module obtained for aminophylline can reduce the cell’s ability to bind oxygen and transport it through capillaries.

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Section: Discussionmentioning

confidence: 99%

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Żuk¹,

Targosz‐Korecka²,

Szymoński³

2011

IJN

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“…1B, C, D). This contribution will also bring together and strengthen different concepts related to the evolution of the RBC function, which we were developing and are scattered around in several previous publications [3][4][5][6]. The treatment will be restricted to red blood cells without nuclei, such as the human red blood cell.…”

Section: Introductionmentioning

confidence: 74%

“…3C. In fact, there is only partial correspondence between the shape behavior of RBC and that of phospholipid vesicles [6]. The stress-free shape of the RBC is a discocyte and thus identical to the shape of a vesicle with the same reduced volume.…”

Section: The Rbc Volume To Membrane Area Ratio Is Optimalmentioning

confidence: 98%

Red blood cell shape and deformability in the context of the functional evolution of its membrane structure

Svetina

2012

Cellular and Molecular Biology Letters

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It is proposed that it is possible to identify some of the problems that had to be solved in the course of evolution for the red blood cell (RBC) to achieve its present day effectiveness, by studying the behavior of systems featuring different, partial characteristics of its membrane. The appropriateness of the RBC volume to membrane area ratio for its circulation in the blood is interpreted on the basis of an analysis of the shape behavior of phospholipid vesicles. The role of the membrane skeleton is associated with preventing an RBC from transforming into a budded shape, which could form in its absence due to curvature-dependent transmembrane protein-membrane interaction. It is shown that, by causing the formation of echinocytes, the skeleton also acts protectively when, in vesicles with a bilayer membrane, the budded shapes would form due to increasing difference between the areas of their outer and inner layers.

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“…The experimental findings are certainly of interest as they indicate that auranofin affects the human erythrocyte morphology. It must be considered that alteration of the normal biconcave shape of red blood cells increases their resistance to entry into capillaries, which could contribute to decreased blood flow, loss of oxygen, and tissue damage through microvascular occlusion 23,24 .…”

Section: Discussionmentioning

confidence: 99%

Structural Effects of the Au(i) Drug Auranofin on Cell Membranes and Molecular Models

Suwalsky¹,

González²,

Villena³

et al. 2013

J. Chil. Chem. Soc.

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Auranofin is a gold compound that is widely used for the treatment of rheumatoid arthritis. It is a monomeric linear complex with triethylphosphine and thiolate moieties bounded to an Au(I) center. Gold compounds are well known for their neurological and nephrotoxic implications. However, haematological toxicity is one of the most serious toxic and less studied effects. The lack of information on these aspects of auranofin prompted us to study the structural effects induced on cell membranes, particularly that of human erythrocytes. Auranofin was incubated with intact erythrocytes and molecular models of the erythrocyte membrane. The latter consisted of multibilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), phospholipids classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. This report presents evidence in order that auranofin interacts with red cell membranes as follows: a) in scanning electron microscopy studies on human erythrocytes it was observed that auranofin induced shape changes; b) X-ray diffraction studies showed that auranofin induced increasing structural perturbation to DMPC and to a lower extent to DMPE bilayers. Additional experiments were performed in human neuroblastoma cells SH-SY5Y. A statistically significant decrease of cell viability was observed.

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The cooperative role of membrane skeleton and bilayer in the mechanical behaviour of red blood cells

Cited by 67 publications

References 27 publications

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Effect of selected drugs used in asthma treatment on morphology and elastic properties of red blood cells

Red blood cell shape and deformability in the context of the functional evolution of its membrane structure

Structural Effects of the Au(i) Drug Auranofin on Cell Membranes and Molecular Models

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